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1,4-Dihydropyridine receptor associated with Ca2+ channels in human embryonic fibroblasts 总被引:2,自引:0,他引:2
By using the radioactively labeled 1,4-dihydropyridine (DHP) probe, [3H]PMD, we have demonstrated that cultured human embryonic fibroblasts grown at a low density in Eagle's medium supplemented with serum contain a single class of non-interacting DHP binding sites (Bmax, 1.2 +/- 0.3 pmol/10(6) cells; Kd, 3.9 nM). After inhibition of the DHP receptor biosynthesis by cycloheximide, the number of [3H]PMD binding sites is reduced with a half-time of 12 h, which implies a turnover rate of 30,000 +/- 7500 receptors/h per cell. With progression to confluency, the Bmax value decreased up to 0.28 +/- 0.08 pmol/10(6) cells without significant change in Kd value. When cells were grown at a low density in serum-free conditions, the number of [3H]PMD binding sites gradually increased 1.9-fold within 3 days. Addition of serum reversed this effect with the same time course. These results imply that the DHP-sensitive Ca2+ channels are involved in the control of the proliferation of human embryonic fibroblasts. 相似文献
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Chernyuk D. P. Bol’shakova A. V. Vlasova O. L. Bezprozvanny I. B. 《Journal of Evolutionary Biochemistry and Physiology》2021,57(2):289-303
Journal of Evolutionary Biochemistry and Physiology - The Morris Water Maze (MWM) behavioral test is a universal method for testing cognitive functions in experimental rodents, and it is especially... 相似文献
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