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1.
Stopped-flow kinetic studies of the formation of ferrioxamine B were performed. Formation of the complex follows the rate law where Ka is the acid dissociation constant of the iron(III) aquo species in 0.1 M formate buffer. At 25°C k1 = 3.94 × 102M?1 sec?1, k2Ka = 1.18 × 10?1 sec?1, k3 = 3.6 × 10?1 sec?1. Activation parameters for k1 are ΔH≠ = 11.7 kcal mole?1 and ΔS≠ = ?8 cal K?1 mole?1. An associative mechanism is proposed. Attachment of the first chelate ring is the slow step and favorably positions the second chelate ring for attachment. Coordination of two chelate rings favorably positions the third chelate ring for attachment. These results are compared to kinetics of formation of model complexes and to a previous study of the formation of ferrioxamine B in which attachment of the third chelate ring was proposed as the slow step 相似文献
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A composting experiment was conducted to evaluate the effect of a hyperlignocellulolytic fungal consortium and different nitrogen
amendments on paddy straw composting in terms of changes in physicochemical and biological parameters. A fungal consortium
comprising four lignocellulolytic mesophilic fungal cultures was used as inoculum for bioaugmentation of paddy straw in perforated
pits. The comparative effect of farmyard manure (FYM), soybean trash, poultry litter and urea on the composting process was
evaluated at monthly intervals in terms of physicochemical (pH, EC, available P, C:N ratio and humus content) and biological
(enzymatic and microbial activity) parameters. The compost prepared from bioaugmented paddy straw composting mixture, with
poultry manure as nitrogen supplement attained desirable C:N ratio in 1 month and displayed least phytotoxicity levels along
with higher production of β-1,4-Exoglucanase. The combined activity of the autochthonous composting microbiota as well as the externally applied fungal
inoculum accelerated the composting process of paddy straw. Supplementation of paddy straw with poultry manure in 8:1 ratio
was identified as the best treatment to hasten the composting process. This study highlights the importance of application
of fungal inoculum and an appropriate N-amendment such as poultry manure for preparation of compost using a substrate having
high C:N ratio, such as paddy straw. 相似文献
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The ferric uptake regulator (Fur) is a metal-dependent DNA-binding protein that acts as both a repressor and an activator of numerous genes involved in maintaining iron homeostasis in bacteria. It has also been demonstrated in Vibrio cholerae that Fur plays an additional role in pathogenesis, opening up the potential of Fur as a drug target for cholera. Here we present the crystal structure of V. cholerae Fur that reveals a very different orientation of the DNA-binding domains compared with that observed in Pseudomonas aeruginosa Fur . Each monomer of the dimeric Fur protein contains two metal binding sites occupied by zinc in the crystal structure. In the P. aeruginosa study these were designated as the regulatory site (Zn1) and structural site (Zn2). This V. cholerae Fur study, together with studies on Fur homologues and paralogues, suggests that in fact the Zn2 site is the regulatory iron binding site and the Zn1 site plays an auxiliary role. There is no evidence of metal binding to the cysteines that are conserved in many Fur homologues, including Escherichia coli Fur. An analysis of the metal binding properties shows that V. cholerae Fur can be activated by a range of divalent metals. 相似文献
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Anis Ahamed Ibrahim A. Arif Mohammed Mateen Radhakrishnan Surendra Kumar Akbar Idhayadhulla 《Saudi Journal of Biological Sciences》2018,25(6):1227-1235
A new series of 1,4-dihydropyridine derivatives (2a–h, 3a–e, and 4a–e) were systematically designed and synthesized via ultrasound irradiation methods with easy work-up and good yields. Compounds structures were confirmed by IR, 1H NMR, 13C NMR, and mass spectra. The synthesized compounds were screened for both antimicrobial and anticoagulant activities. Compound 2e (MIC: 0.25?μg/mL) was highly active against Escherichia coli and compound 2c (MIC: 0.5?μg/mL) was also highly active against Pseudomonas aeruginosa compared with ciprofloxacin. (MIC: 1?μg/mL) The antifungal activity of 2c (MIC: 0.5?μg/mL) against Candida albicans was high relative to that of clotrimazole (MIC: 1?μg/mL). Anticoagulant activity was determined by activated partial thromboplastin time (APTT) and prothrombin time (PT) coagulation assays. Compound 4-(4-hydroxyphenyl)-2,6-dimethyl-N3,N5-bis(5-phenyl-1,3,4-thiadiazol-2-yl)-1,4-dihydropyridine-3,5-dicarboxamide 3d (>1000?s in APTT assays) was highly active in anticoagulant screening compared with the reference of heparin.Cytotoxicity was evaluated using HepG2 (liver), HeLa (cervical), and MCF-7 (breast) cancer cell lines, with high toxicities observed for 2c (GI50?=?0.02?μm) against HeLa cell line and 2e (GI50?=?0.03?μm) equipotant against MCF-7 cell line. Therefore, the compounds 2e, 2c and 3d can serve as lead molecules for the development of new classes of antimicrobial and anticoagulant agent. 相似文献
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Qun Li Zhiyuan Han Navleen Singh Berta Terr Ryann M. Fame Uzayr Arif Thomas D. Page Tasneem Zahran Ahmed Abdeltawab Yuan Huang Ping Cao Jun Wang Hao Lu Hart G. W. Lidov Kameswaran Surendran Lizhao Wu James Q. Virga Ying-Tao Zhao Ulrich Schüller Robert J. Wechsler-Reya Maria K. Lehtinen Sudipto Roy Zhongmin Liu Travis H. Stracker Haotian Zhao 《Cell death and differentiation》2022,29(8):1596
9.
Mohammad Badrul Anam Arif Istiaq Ryusho Kariya Mikiko Kudo Shah Adil Ishtiyaq Ahmad Naofumi Ito Seiji Okada Kunimasa Ohta 《Biochemistry and Biophysics Reports》2021
Previously we reported that, lactic acid bacteria (LAB) can induce human dermal fibroblast (HDF) cells to form multipotent cell clusters which are able to transdifferentiate into three germ layer derived cell lineages. Later on, we confirmed that ribosome is responsible for the LAB-induced transdifferentiation and ribosomes from diverse organisms can mimic the LAB effect on HDF cells. In our present study we have shown that, upon incorporation of ribosomes, non-small cell lung cancer cell line A549 and gastric tubular adenocarcinoma cell line H-111-TC are transformed into spheroid like morphology those can be transdifferentiated into adipocytes and osteoblast. Our qPCR analysis has revealed that, during the formation of ribosome induced cancer cell spheroids, the expression of the cancer cell associated markers and cell cycle/proliferation markers were altered at different time point. Through our investigation, here we report a novel and a non-invasive approach for cancer cell reprogramming by incorporating ribosomes. 相似文献
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