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1.
Peroxidation of phosphatidylcholine and cholesterol in mixed monolayers at the air-water surface is studied. It is shown that the rate of phosphatidylcholine peroxidation is abruptly decreased in the presence of cholesterol. 相似文献
2.
Chasovnikova OB Mitrofanov DV Anisimenko MS Voevoda MI Kovalenko SP Liakhovich VV 《Genetika》2012,48(6):768-772
Frequencies of the 538insC mutation in the BRCA1 gene and the 1100delC mutation in the CHEK2 gene were compared in the group of breast cancer patients and the large-scale sample, consisting of 7920 DNA specimens from healthy residents of the city of Novosibirsk. Higher frequencies of these mutations in the patient group compared to the control sample (1.95 versus 0.25% for BRCA1 5382insC, and 1.78 versus 0.40% for CHEK2 1100delC) were observed, pointing to their association with susceptibility to breast cancer (OR = = 7.86, 95% CI 3.51-17.30 and OR =4.46, 95% C1 2.04-9.49, respectively). 相似文献
3.
D. V. Mitrofanov O. B. Chasovnikova L. S. Koroleva V. N. Silnikov L. G. Zhdanova S. P. Kovalenko 《Russian Journal of Genetics》2008,44(12):1461-1467
A simple method was developed for end-point fluorescence detection of the 735G → A mutation of the 5′-splice donor site of intron 14 of the dihidropyrimidine dehydrogenase gene (DPYD). The method was based on allele-specific PCR with duplex Scorpion primers. The genotyping results obtained by the fluorescent end-point PCR technique completely coincided with the results obtained by allele-specific PCR with amplicon detection in agarose gel. Genotyping was performed in 291 DNA samples from residents of Novosibirsk region (Russia), and two heterozygotes (0.69%) were detected. 相似文献
4.
M A Pal'tsev Iu M Esilevski? N N Chasovnikova L M Shumkina 《Biulleten' eksperimental'no? biologii i meditsiny》1982,93(3):119-122
Electron microscopy was used to examine the status of the juxtaglomerular apparatus (JGA) and interstitial cells (IC) 3 and 24 hours after administration of furosemide (10 mg/kg), indomethacin (10 mg/kg), venoruton (500 mg/kg) and trental (100 mg/kg), and after 1,6 an 12 sessions of hyperbaric oxygenation. To evaluate objectively the results of examining the JGA, use was made of a method devised by the authors of a mathematical appraisal of granulation from the density of the epithelioid cells. Granulation of 50 IC from each animal was calculated on semi-thin araldite sections stained methylene blue-azur II-fuchsin. The results indicate that all the types of exposure including hyperbaric oxygenation produced JGA activation whose degree varied depending on the time elapsed after exposure. An apparently great increase in the JGA activity was detected after injection of furosemide and indomethacin. All the drugs with the exception of furosemide entailed granule accumulation after 3 hours, followed by the recovery of their amount after 24 hours. Furosemide injection produced a reverse effect. 相似文献
5.
T M Makarova E I Varlinskaia T I Chasovnikova E S Petrov 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》1991,41(6):1231-1238
It is shown, that rats, bred in conditions of intraspecies isolation are able to competition for water in the kin group, having usual experience of intraspecies intercourse. At the same level of drinking motivation in conditions of competition for water the intraspecies activity is significantly higher in rats, bred in isolation in comparison with grouped animals. Rats-isolants significantly more often than the grouped animals use extraordinary tactics of intraspecies behaviour, which increase their competitive ability in conditions of limited access to water. 相似文献
6.
D. V. Mitrofanov O. B. Chasovnikova S. P. Kovalenko V. V. Lyakhovich 《Molecular Biology》2009,43(6):930-936
Analysis of genetic predisposition to cancer provides valuable information for early cancer detection or even prevention.
The 5382insC insertion in the BRCA1 gene is the most frequent mutation among those associated with high breast cancer risk in women of East European origin.
A method for 5382insC detection with fluorescent labeled allele-specific oligonucleotides in the Duplex Scorpion format has
been developed. The method can be used under real-time PCR conditions as well as under the conditions of end-point fluorescence
measurement following regular PCR. The adequacy of the method was demonstrated in the study of the 5382insC mutation frequency
in breast cancer patients. A total of 564 genomic DNA samples from breast cancer patients were screened. Eleven patients (1.95%)
were found to carry the mutation under consideration in the heterozygous state. The 5382insC allele frequency in the breast
cancer patient group was 0.0098. The method is appropriate in clinical practice to detect individuals at high breast cancer
risk, as well as in large-scale population studies. 相似文献
7.
Paulo HG Mansur Lacordaire KP Cury José OB Leite Adriano A Pereira Nilson Penha-Silva Adriano O Andrade 《Biomedical engineering online》2010,9(1):29
Background
The main problem of tremor is the damage caused to the quality of the life of patients, especially those at more advanced ages. There is not a consensus yet about the origins of this disorder, but it can be examined in the correlations between the biological signs of aging and the tremor characteristics. 相似文献8.
L V Chasovnikova N A Matveeva A K Kurek V A Aleshkin V V Lavrent'ev 《Molekuliarnaia genetika, mikrobiologiia i virusologiia》1986,(11):36-40
Pathological immunoglobulins (IgG from patients with multiple myeloma and IgM from patients with Waldenstr?m macroglobulinemia) have been shown to possess hydrophylic-lipophylic balance (HLB) which differed from normal Ig HLB. HLB deficiency in pathological proteins was due to the increase of hydrophobic area at the surface of protein globe, which was the reason for different normal and abnormal Ig orientations at the aqueous NaCl solution--air interface. The normal IgG and IgM had horizontal orientation while abnormal ones had vertical orientation. Both normal and abnormal Ig changed their orientation in monolayers as a result of sodium deoxycholate processing. The change in orientation depended on protein molecules interaction with single molecules or micelles of sodium deoxycholate. 相似文献
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10.
Human secretory immunoglobulin A (IgA) resistance to surface denaturation as a function of NaCl concentration and pH of aqueous phase has been studied by monomolecular layer method at air-water interface. Ig A has been shown to form monolayers of native molecules oriented horizontally towards interface in pH range from 5.2 to 9.0 and NaCl concentration from 0.15 M to 0.65 M. Beyond these regions surface denaturation rate in directly proportional to H+ and NaCl concentration. It was established that Ig A molecule conformational stability was higher than that of IgG. 相似文献