全文获取类型
收费全文 | 1223篇 |
免费 | 116篇 |
国内免费 | 2篇 |
出版年
2023年 | 7篇 |
2022年 | 12篇 |
2021年 | 15篇 |
2020年 | 15篇 |
2019年 | 17篇 |
2018年 | 20篇 |
2017年 | 17篇 |
2016年 | 41篇 |
2015年 | 45篇 |
2014年 | 49篇 |
2013年 | 73篇 |
2012年 | 93篇 |
2011年 | 101篇 |
2010年 | 58篇 |
2009年 | 52篇 |
2008年 | 69篇 |
2007年 | 61篇 |
2006年 | 65篇 |
2005年 | 65篇 |
2004年 | 76篇 |
2003年 | 72篇 |
2002年 | 41篇 |
2001年 | 16篇 |
2000年 | 8篇 |
1999年 | 12篇 |
1998年 | 16篇 |
1997年 | 14篇 |
1996年 | 11篇 |
1995年 | 17篇 |
1994年 | 5篇 |
1993年 | 11篇 |
1992年 | 22篇 |
1991年 | 9篇 |
1990年 | 13篇 |
1989年 | 5篇 |
1988年 | 12篇 |
1987年 | 14篇 |
1986年 | 7篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1981年 | 10篇 |
1980年 | 5篇 |
1978年 | 4篇 |
1974年 | 9篇 |
1970年 | 3篇 |
1968年 | 3篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1965年 | 9篇 |
1964年 | 5篇 |
排序方式: 共有1341条查询结果,搜索用时 46 毫秒
1.
2.
Catherine Bonaïti-Pellié Françoise Clerget-Darpoux Marie-Claude Babron 《Human genetics》1990,86(2):203-208
Summary Although the retinoblastoma gene has been isolated and sequenced, the difference in penetrance and expressivity among families has not yet been fully explained. Balanced chromosomal insertion involving the 13q14 regions has been shown to account for some families with several unaffected carriers. Since there could be cases with karyotypically undetectable insertions, we tested whether this mechanism was general enough to explain the whole difference in expressivity among families. Using 166 pedigrees, reported in nine series available in the literature (including our own), we conclude that balanced insertion cannot entirely explain the familial data, even if we allow for a reduced viability of unbalanced gametes. Other mechanisms are proposed and discussed in this paper. 相似文献
3.
Successful pregnancy in a woman with congenital factor XIII deficiency treated with substitutive therapy. Report of a second case 总被引:1,自引:0,他引:1
A syndrome of marked fetal wastage is associated with congenital factor XIII deficiency in adult women. A previously unreported case of a woman with factor XIII deficiency is described, in which substitutive treatment with normal plasma or placental factor XIII concentrate permitted two normal pregnancies. Factor XIII activity was maintained above 1-2% with intermittent infusion of 300 ml to 450 ml of plasma every 14 days or of 500 units of concentrate every 21 days. This case confirms the only other case so far reported in which factor XIII substitutive therapy was able to permit a normal pregnancy in a woman with factor XIII deficiency and seems to suggest factor XIII to be involved in the process of annidation. 相似文献
4.
The enzyme dipeptidase-A (DIP-A) in Drosophila melanogaster is coded by a second chromosome locus that is polymorphic for three allozymes in natural populations. DIP-A appears to be the only enzyme in D. melanogaster capable of hydrolyzing the dipeptide glycyl-L-isoleucine, since flies homozygous for null alleles at this locus have no detectable glycyl-L-isoleucine-ase activity. DIP-A activity occurs in many tissues and throughout development, but is particularly high in the larval midgut, suggesting an important role in protein digestion. These observations suggested an experimental design for investigating the adaptive significance of genetic variation in DIP-A activity. Fitness components of DIP-A variants could be estimated and compared under two environmental conditions (defined diets under axenic conditions). In the restrictive environment, the essential amino acid L-isoleucine is provided only in the form of glycyl-L-isoleucine, whereas in the permissive environment, L-isoleucine is provided in free form. We predicted that DIP-A activity would be essential in the restrictive, but not in the permissive environment. The results reported here clearly contradict this prediction. Two stocks homozygous for DIP-A null alleles from different geographic locations are each viable on the restrictive diet. Furthermore, relative viability experiments in which null allele larvae compete with larvae having DIP-A activity provide no evidence for even a partial reduction in egg to adult survival on the restrictive diet. Apparently, the null allele larvae have some alternative mechanism for obtaining L-isoleucine from the dipeptide, even though no glycyl-L-isoleucine-ase activity can be detected in vitro. These results, along with the viability of null alleles for many other enzymes, support the idea that eukaryotes have an intricate network of alternative biochemical pathways through which the same necessary function may be achieved. Such "buffering capacity" makes it very difficult to analyze the effects of enzyme variants on fitness components. 相似文献
5.
Mapping of the structural gene for S-adenosyl homocysteine hydrolase to mouse Chromosome 2, and related sequences to Chromosomes 8 and X 总被引:1,自引:0,他引:1
Alison Pilz Paul Le Tissier Heather Moseley Jo Peters Cathy Abbott 《Mammalian genome》1992,3(11):633-636
Comparative mapping studies in human and mouse have shown that, to date, human Chromosome (Chr) 20 is completely syntenic with distal mouse Chr 2. The structural locus for S-adenosyl-l-homocysteine hydrolase (EC 3.3.1.1) in human, AHCY, maps to 20 qterq13.1, and we report here that the homologous locus in the mouse, Ahcy, maps to distal mouse Chr 2 with gene order Pcna-Ahcy-Ada. Analysis of 123 progeny of an interspecific backcross between a laboratory stock, AN, and Mus spretus using a rat cDNA probe revealed the presence of at least two other Ahcy-related sequences segregating independently in the mouse genome. One, Ahcy-rs1, was mapped to Chr 8 in the BXH recombinant inbred strains, and the other, Ahcy-rs2, shows a pattern of inheritance consistent with X-linkage. 相似文献
6.
7.
Internal antigen and immune network 总被引:5,自引:0,他引:5
H Kohler S Muller C Bona 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1985,178(2):189-195
The network hypothesis postulates the existence of internal idiotopic structures which mimic nominal antigens. Experimental evidence for idiotope internal antigen is presented and its implication for the Network hypothesis is discussed. The exploitation of the internal idiotope antigens (or preparation of vaccines) is a realistic possibility. 相似文献
8.
9.
Dilip M. Shah Cathy M. Hironaka Roger C. Wiegand Elizabeth I. Harding Gwen G. Krivi David C. Tiemeier 《Plant molecular biology》1986,6(4):203-211
Summary We have used the cDNA clone encoding maize glutathione-S-transferase (GST I) to isolate a genomic DNA clone containing the complete GST I gene. Nucleotide sequence analysis of the cDNA and genomic clones has yielded a complete amino acid sequence for maize GST I and provided the exon-intron map of its gene. The mRNA homologous sequences in the maize GST I gene consist of a 107 bp 5 untranslated region, a 642 bp coding region and 340 bp of the 3 untranslated region. They are divided into three exons by two introns which interrupt the coding region. The 5 untranslated spacer contains an unusual sequence of pentamer AGAGG repeated seven times. The inbred maize line (Missouri 17) contains a single gene for GST I, whereas the hybrid line (3780A) contains two genes. Nucleotide sequence analysis of the primer extended cDNA products reveals that the 5 untranslated regions of the two genes in the hybrid 3780A are identical except for a 6 bp internal deletion (or insertion). The amino acid sequence of maize GST I shares no apparent sequence homology with the published sequences of animal GST's and represents the first published sequence of a plant GST. re]19850813 ac]19851126 相似文献
10.