排序方式: 共有16条查询结果,搜索用时 15 毫秒
1.
M. Calomme J. Hu K. Van Den Branden D. A. Vanden Berghe 《Biological trace element research》1995,47(1-3):379-383
Lactic acid bacteria are nonpathogenic bacteria commonly used in food processing. An evaluation was made of the capacity to
concentrate selenium in species of Lactobacillus. A selenium concentration of 1 μg/mL in the culture medium yielded in a bacterial
content of 400 μg/g dry biomass. Dialysis and TCA precipitation experiments of a native intracellular extract proved that
at least 80% of the total selenium is associated with organic molecules. Seleno-cysteine was identified as the only seleno-amino
acid present in the intracellular selenoproteins. This study shows that species of the lactic acid bacteria are able to concentrate
selenium intracellular as seleno-cysteine, which could be applied in supplementation studies. 相似文献
2.
Mario Calomme Jean Vanderpas Baudouin François Micheline Van Caillie-Bertrand Nicole Vanovervelt Christian Van Hoorebeke Dirk Vanden Berghe 《Biological trace element research》1995,47(1-3):349-353
Type I 5′-deiodinase was recently characterized as a selenocysteine-containing enzyme in humans and other mammals. Up to now,
the effect of selenium (Se) supplementation on thyroid hormone metabolism in humans has only been reported in the very peculiar
nutritional environment of Central Africa, where combined severe iodine and Se deficiency occurs. In this study, a group of
phenylketonuria subjects with a low selenium status, but a normal iodine intake were supplemented with selenium to investigate
changes in their thyroid hormone metabolism. After 3 wk of selenium supplementation (1 μg/kg/d), both the concentrations of
the prohormone thyroxine (T4) and the metabolic inactive reverse triiodothyronine (rT3) decreased significantly. Clinically,
the phenylketonuria subjects remained euthyroid before and after selenium supplementation. The individual changes of plasma
Se and glutathione peroxidase activity were closely associated with individual changes of plasma T4 and rT3. 相似文献
3.
Nguyên TL Calomme C Wijmeersch G Nizet S Veithen E Portetelle D de Launoit Y Burny A Van Lint C 《The Journal of biological chemistry》2004,279(33):35025-35036
4.
P Rubiolo L Pieters M Calomme C Bicchi A Vlietinck D Vanden Berghe 《Mutation research》1992,281(2):143-147
The mutagenicity of a series of pyrrolizidine alkaloids, and of extracts from several Italian Senecio species containing pyrrolizidine alkaloids, including S. inaequidens, S. fuchsii and S. cacaliaster, were tested using the Salmonella typhimurium/mammalian microsome system. Retrorsine, senecivernine, seneciphylline and the Senecio extracts showed a weakly mutagenic activity. 相似文献
5.
Overlapping CRE and E box motifs in the enhancer sequences of the bovine leukemia virus 5' long terminal repeat are critical for basal and acetylation-dependent transcriptional activity of the viral promoter: implications for viral latency 下载免费PDF全文
Calomme C Dekoninck A Nizet S Adam E Nguyên TL Van Den Broeke A Willems L Kettmann R Burny A Van Lint C 《Journal of virology》2004,78(24):13848-13864
6.
Selenium and Lactobacillus species 总被引:1,自引:0,他引:1
M. R. Calomme K. Van den Branden D.A. Vanden Berghe 《Journal of applied microbiology》1995,79(3):331-340
Selenium (Se) is an essential element for man and animal. Supplementation of Se has been done with Se-enriched yeast and inorganic Se compounds. In the present study a quantitative and qualitative evaluation was made of whether lactobacilli are able to concentrate Se. A high correlation was found between the bacterial Se concentration and the concentration of Se in the medium. The Se concentration in biomass was respectively 253 ± 50, 375 ± 33 and 407 ± 108 μg g−1 dry weight for Lactobacillus delbrueckii subsp. bulgaricus, Lact. plantarum and Lact. casei subsp. casei when 1 mg 1−1 Se4+ was present in the medium. Manganese (Mn) was concentrated in Lact. plantarum and Lact. casei subsp. casei but not in Lact. delbrueckii subsp. bulgaricus. The Mn concentration in biomass was higher compared to the Se concentration but this difference decreased when the concentration of Mn/Se increased in the culture medium. Copper, zinc and iron were also concentrated in biomass of Lact. delbrueckii subsp. bulgaricus. Characterization of the bacterial selenocompound revealed that 75 Se was generally incorporated, as selenocysteine, into protein of Lact. delbrueckii subsp. bulgaricus. Addition of L-cysteine to the medium decreased the bacterial Se content. It was concluded that Lact. delbrueckii subsp. bulgaricus incorporated Se non-specifically into bacterial protein. The application of Se-enriched lactobacilli (Se-Lb) in Se supplementation would be an interesting approach since selenomethionine, which is the major selenocompound in commercialized Se-yeast, was not detected in Se-Lb and because lactobacilli are already widely used in human nutrition. 相似文献
7.
Suboptimal Enhancer Sequences Are Required for Efficient Bovine Leukemia Virus Propagation In Vivo: Implications for Viral Latency 下载免费PDF全文
8.
9.
Plant Cell, Tissue and Organ Culture (PCTOC) - Mineral nutrients are necessary for the growth and development of plants. Previous studies have concentrated mainly on silicon-accumulating plants,... 相似文献