A TAXONOMIST'S VIEW OF THE COLI-AEROGENES BACTERIA

SUMMARY: Examples are given of different names that might be applied to the same organisms when the scheme of classification is changed. In particular, the classification of the Coli-Aerogenes (1956) Sub-Committee of the Society for Applied Bacteriology and the proposed nomenclature are discussed.     

The BULT Melanoma: A Spontaneous Transplantable Tumor in Mice

The BULT melanoma originated at Brown University as a spontaneous, small black nodule on the tail of an adult female mouse of the LT/Ch strain. Histological examination of a portion of the tumor indicated that it was intradermal and consisted predominantly of heavily melanized, ovoid to fusiform cells with melanin-laden macrophages scattered among them. The BULT melanoma has been maintained in LT/Ch mice for approximately 5 years by periodic transplantation, at first subcutaneously on the flanks and, more recently, intramuscularly in the hind legs. The shift in transplantation site was made following a marked decline in the growth of subcutaneous grafts. The transplants have retained the uniform deep-black melanization and general histology of the primary melanoma. Numerous melanosomes at all stages of development are found within the melanoma cells. DOPA-positive cytoplasmic vesicles are abundant. Occasional autophagic vacuoles containing clusters of melanosomes are also present. A few metastases from the transplanted melanoma have been observed in lymph nodes and on one occasion in the lungs. When grown in vitro, BULT melanoma cells do not require special growth promoting agents (e.g., TPA; cAMP) in order to proliferate. The BULT melanoma differs in one or more respects from each of the other three transplantable spontaneous mouse melanomas widely used in cancer research. In addition, it arose in a strain of mice characterized by the spontaneous death of melanocytes while the latter are engaged in synthesizing eumelanin within hair follicles. Karyotypic analysis of cultured cells showed a modal chromosome number of 68 with a range of 58–72 chromosomes.     

The new-object and new-place reactions of Rattus rattus L

The behaviour of the wild "black" or "house" rat, Rattus rattus L., was studied in an artificial environment, a plus-maze. The maze consisted of a central nest box and four arms. Food and water were placed at the ends of different arms, and movements of the rats were recorded automatically. A detailed analysis was made of habituation to the artificial environment and of the patterns of activity displayed there. Rats were most active during the dark phase of 'he light cycle; they displayed a bimodal pattern of visits to and time spent in the arms, with peaks around three hours after lights off and four hours before lights on. Reaction to new objects and places was measured in several ways. The rats usually avoided new objects placed in a familiar area, but showed little hesitation to explore a previously inaccessible area. Rats generally stopped avoiding new objects in the feeding area within one to two days, even when an alternative source of food was available elsewhere. There was, however, much variation.
The methods used allow study of two distinct initial responses to novelty, each clearly displayed: (a) withdrawal from a strange object in a familiar place ("fear"), and (b) approach to a newly accessible area ("curiosity"). Behaviour often depends on the interaction between these two processes.     

An Evaluation of Diazacon as a Potential Contraceptive in Non-Native Rose-Ringed Parakeets

ABSTRACT Rose-ringed parakeets (Psittacula krameri) have become widely established outside their native range through accidental or deliberate release. Potential economic impacts on agriculture, conservation concerns, and mixed public opinion regarding the species have highlighted the need to develop effective but humane management options. Fertility control might provide such a solution if a safe and environmentally benign contraceptive was available. The chemical 20,25-diazacholesterol dihydrochloride (diazacon) has previously been used to reduce reproductive output in avian species through reduction of blood cholesterol and cholesterol-dependent reproductive hormones. We orally dosed captive rose-ringed parakeets with a solution of either 9 mg/kg or 18 mg/kg of diazacon for up to 10 days and found that a dose of 18 mg/kg for 10 days temporarily reduced blood cholesterol levels with no adverse side effects. We evaluated this dose level in a captive population in semi-natural conditions during the 2008 breeding season and found a significant decrease in fertility. We concluded that diazacon has potential for fertility control in this species if a suitable formulation and delivery system is developed for free-living populations.     

The Boar-Operated-System: a Novel Method to Deliver Baits to Wild Pigs

ABSTRACT Bait-delivered pharmaceuticals, increasingly used to manage populations of wild boar (Sus scrofa) and feral pigs, may be ingested by nontarget species. Species-specificity could be achieved through a delivery system. We designed the BOS^TM (Boar-Operated-System) as a device to deliver baits to wild pigs. The BOS^TM consists of a metal pole onto which a round perforated base is attached. A metal cone with a wide rim slides up and down the pole and fully encloses the base onto which the baits are placed. We conducted a pilot, captive trial and found that captive wild boar fed from the BOS^TM either directly, by lifting the cone, or indirectly, by feeding once another animal had lifted the cone. Thus, we tested whether free-living wild boar fed from the BOS^TM and whether the BOS^TM could prevent bait uptake by nontarget species. We observed that free-living wild boar fed regularly from the BOS^TM and that the device successfully prevented bait uptake by nontarget species. The BOS^TM should be trialed more extensively to confirm its effectiveness and species-specificity to distribute pharmaceuticals to wild suids. If successful, the BOS^TM could be used to deliver vaccines in disease control programs as well as contraceptives to manage overabundant populations of wild suids.     

Modeling Historical Ecology, Thinking about Contemporary Systems

One way to make archaeological trajectories compelling to contemporary audiences is through detailed computer simulation with graphical interfaces that allow students or researchers to explore historical sequences and ask "what if" questions of them. We discuss progress on modeling coupled human systems and ecosystems over long periods in the Mesa Verde region of the U.S. Southwest, a project developed with both research and educational products in mind. The project has many foci, but here we concentrate on fuel-wood availability and its pre-Hispanic use in the pygmy forests of this region. Our preliminary results tend to indicate that wood for fuels became sufficiently limited in this sequence to become a factor of considerable importance in decisions about where to live, how to build, and even whether to remain in the region. The same logic of modeling can be used to study problems of contemporary forest use in other parts of the world.