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L A Bynoe S Pou J D Gottsch G M Rosen 《Biochemical and biophysical research communications》1991,179(3):1305-1310
The generation of reactive oxygen species from human erythrocytes has previously been demonstrated. Furthermore, erythrocytic protoporphyrin IX has been shown to generate superoxide and singlet oxygen when exposed to light. These findings suggest that a component of erythrocytic reactive oxygen species production may be light-dependent. By inhibiting erythrocyte superoxide dismutase, catalase, and glutathione peroxidase with N,N-diethyldithiocarbamate or sodium cyanide, we demonstrate the light-dependent generation of hydroxyl radical in human erythrocytes using spin trapping/Electron Spin Resonance spectroscopy. This finding may be significant in tissues where blood is exposed to light, such as in the eye. 相似文献
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To understand the nature of sediment-associated nutrient and contaminant transport dynamics in fluvial systems, a stormflow
sampling program of suspended solids is reported for one water year in a representative rural diffuse source catchment of
southeastern Ontario. Bulk samples of subsieve suspended solids were obtained using field-portable continuous-flow centrifuge
apparatus. The physical and geochemical properties of suspended solids show no significant intersite differences over reaches
of 1 500–2 000 m, yet display distinctive seasonal trends. Systematic seasonal changes in particle size, organic content,
and Ca, P, Mn, Al, Ti, Fe, and K appear to reflect the changing role of partial area hydrology. Ca, P, and Mn are bioaccumulated
by stream algae. Mineral signature is relatively constant over the year.
This article first appeared in the Canadian Journal of Earth Sciences. Volume 18, pp. 1365–1379 (1981). Permission of the
copyright holders to reprint the article is gratefully acknowledged. 相似文献
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The attenuation of two human influenza A viruses has been carried out, using the selection of inhibitor-resistant strains and multiple passages at low temperatures. A virus related to A2/Tokyo/3/67 was obtained in an inhibitor-resistant form. When this was compared with the inhibitor-sensitive strain in a volunteer trial it was relatively non-pathogenic. The second virus, A2/Hongkong/1/68, was subjected to much longer treatment, but nevertheless remained slightly sensitive to serum inhibitor. When given to volunteers it was less pathogenic than before but attenuation was incomplete. A2/Hongkong/1/68 was also modified by passage at low temperatures. Many of these passages are apparently necessary for full attenuation.All attenuated viruses were infective and antigenic. 相似文献
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A. C. C. Joosting B. Head M. L. Bynoe D. A. J. Tyrrell 《BMJ (Clinical research ed.)》1968,4(5624):153-154
Influenza C virus was intranasally administered to volunteers; most were infected and nine developed symptoms of common cold.Increasing titres of serum haemagglutination-inhibiting antibody and complement-fixing antibody were detected. Virus neutralizing activity in nasal secretion was not correlated with either resistance to infection or the occurrence of overt illness. Interferon was detected in the nasal secretions of some subjects. 相似文献
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