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排序方式: 共有73条查询结果,搜索用时 15 毫秒
1.
XANES study of iron displacement in the haem of myoglobin 总被引:1,自引:0,他引:1
A Bianconi A Congiu-Castellano M Dell'Ariccia A Giovannelli P J Durham E Burattini M Barteri 《FEBS letters》1984,178(1):165-170
The XANES (X-ray absorption near edge structure) spectra of deoxy human adult haemoglobin (HbA) and myoglobin (Mb) have been measured at the wiggler beam line of the Frascati synchrotron radiation facility. The XANES are interpreted by the multiple scattering cluster theory. The variations in the XANES between HbA and Mb are assigned to changes in the Fe-porphyrin geometry. 相似文献
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Albertini MC Accorsi A Citterio B Burattini S Piacentini MP Uguccioni F Piatti E 《Biochimie》2003,85(10):963-970
The effects of the exposure to a static magnetic field (sMF) of 0.3 +/- 0.03 T on the Fusarium culmorum were investigated in vitro. sMF inhibition of mycelia growth was accompanied by morphological and biochemical changes. Fungal conidia germination and cell viability were also reduced. We provide evidence of the influence of sMF on Ca(2+)-dependent signal transduction pathways involved in conidia germination. Perturbation of these pathways by adding different compounds (i.e. CaCl(2), phorbol 12-myristate 13-acetate, neomycin, EGTA, LiCl) to the medium, suggested that exposed conidia are unable to mobilise calcium from intracellular stores and that the hindered mechanism may be IP(3)-dependent. 相似文献
4.
The tropane alkaloid (TA) scopolamine is suggested to protect Brugmansia suaveolens (Solanaceae) against herbivorous insects. To test this prediction in a natural environment, scopolamine was induced by methyl jasmonate (MJ) in potted plants which were left 10?days in the field. MJ-treated plants increased their scopolamine concentration in leaves and herbivory decreased. These findings suggest a cause?Ceffect relationship. However, experiments in laboratory showed that scopolamine affect differently the performance of the specialist larvae of the ithomiine butterfly Placidina euryanassa (C. Felder & R. Felder) and the generalist fall armyworm Spodoptera frugiperda (J. E. Smith): the specialist that sequester this TA from B. suaveolens leaves was not negatively affected, but the generalist was. Therefore, scopolamine probably acts only against insects that are not adapted to TAs. Other compounds that are MJ elicited may also play a role in plant resistance against herbivory by generalist and specialist insects, and deserve future investigations. 相似文献
5.
Curzi D Salucci S Marini M Esposito F Agnello L Veicsteinas A Burattini S Falcieri E 《European journal of histochemistry : EJH》2012,56(2):e19
Myotendinous junctions can be easily injured by overloading or trauma, and exercise training may be a way of increasing their resistance to mechanical stress. To this end, we examined herein the morphological changes induced by moderate exercise training in the myotendinous junctions of extensor digitorum longus and gastrocnemius muscles in rats. Twelve Sprague-Dawley rats were used in this investigation. Six of them were trained to run on a treadmill for 1 h/day, 3 days/week over 10 weeks in order for them to achieve a running rate of 25 m/min at the end of the training period. Six age-matched sedentary rats were used as controls. The rats were sacrificed 24 h after the final training session, and the extensor digitorum longum (EDL) and the gastrocnemium were excised; the myotendinous junctions (MTJ) were then prepared and observed with electron microscopy. Digitation branching was evaluated by counting the bifurcations in the MTJ protrusions. Our observations indicate that exercise does indeed induce changes in MTJ morphology. In both muscles the number of bifurcated interdigitations increased significantly, as well as, in gastrocnemius, the branching of the finger-like processes. It was demonstrated that the MTJ is able to adapt to an increase in tensile force by enlarging the muscle-tendon contact area and, consequently, mechanical resistance. 相似文献
6.
Marini M Falcieri E Margonato V Treré D Lapalombella R di Tullio S Marchionni C Burattini S Samaja M Esposito F Veicsteinas A 《Histochemistry and cell biology》2008,129(4):479-487
Enhanced angiogenesis, or capillary growth, has a prominent role among the various beneficial effects of exercise training
on the myocardium. The aim of the present study is to assess if training-induced increases in capillarity and vascularization
persist after 4 weeks of detraining. Adult male rats were trained to run on a treadmill for 10 weeks at ∼60% VO2max, which did not induce cardiac hypertrophy, but increased (P < 0.05) the soleus/body weight ratio, left ventricle capillarity and von Willebrand-positive cell density (n = 6). In another group of animals (n = 6) subjected to training followed by 4-week detraining, the soleus/body weight ratio returned to normal, with only partial
reversal of left ventricle capillarity and von Willebrand-positive cell density. Markers of angiogenesis (VEGF, KDR/VEGF-R2
and HIF-1α mRNA, studied by real-time RT-PCR) were upregulated at the end of training, and returned to baseline value after
detraining. Electron microscopy highlighted some morphological features in trained hearts (endothelial cell sprouting and
bridges and pericyte detachment), suggestive of endothelial cell proliferation and capillary growth that were absent in untrained
and detrained hearts. We conclude that the training-induced increase in cardiac capillarity and vascularization are retained
for some time upon cessation of the training program even in the absence of angiogenic stimuli. 相似文献
7.
Picceri Giada Giusi Leonardi Pamela Iotti Mirco Gallo Michele Baldi Franco Zambonelli Alessandra Amicucci Antonella Vallorani Luciana Piccoli Giovanni Ciccimarra Giovanni Arshakyan Marselina Burattini Sabrina Falcieri Elisabetta Chiarantini Laura 《Applied microbiology and biotechnology》2018,102(3):1429-1441
Applied Microbiology and Biotechnology - Iron exopolysaccharide nanoparticles were biogenerated during ferric citrate fermentation by Klebsiella oxytoca DSM 29614. Before investigating their... 相似文献
8.
MN Kaggwa N Straubinger-Gansberger M Schagerl 《African Journal of Aquatic Science》2018,43(2):123-129
Nine small artificial dams located in different climatic regions of Kenya were studied. The local communities use the stored water for various purposes, such as irrigation, domestic use, watering of livestock and cage fish farming. Such intense use is commonly accompanied by eutrophication, including fast growth of cyanobacteria, which at times produce cyanotoxins threatening human and animal life. We studied the pelagic community, analysed abiotic variables and identified microcystins by means of high performance liquid chromatography and ELISA kits at monthly intervals over a period of one year. Mass spectrometry (MALDI-TOF MS) was used to identify structural variants of microcystins by their protonated masses (M + H). Three dams contained microcystins, with the highly toxic Microcystin-LR being identified as the most prominent substance. Cell content of the toxin varied from 7.2 to 686.7 fg cell?1. Basic limnological variables that indicate the probability of toxin presence were also recorded. Non-parametric Mann–Whitney U-test revealed significant differences in soluble reactive phosphorous, nitrate-N, water depth, total hardness and post-Nauplii stages sampled between toxin-producing and non-toxin-producing dams. Although most of the samples did not contain high amounts of cyanobacteria, the cyanotoxin-problem was evident, suggesting the need for regular cyanotoxin monitoring programs. 相似文献
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Eduardo Massad Annelies Wilder-Smith Raphael Ximenes Marcos Amaku Luis Fernandez Lopez Francisco Antonio Bezerra Coutinho Giovanini Evelim Coelho Jarbas Barbosa da Silva Jr Claudio José Struchiner Marcelo Nascimento Burattini 《Memórias do Instituto Oswaldo Cruz》2014,109(3):394-397
Brazil will host the FIFA World Cup™, the biggest single-event competition in the
world, from June 12-July 13 2014 in 12 cities. This event will draw an estimated
600,000 international visitors. Brazil is endemic for dengue. Hence, attendees of the
2014 event are theoretically at risk for dengue. We calculated the risk of dengue
acquisition to non-immune international travellers to Brazil, depending on the
football match schedules, considering locations and dates of such matches for June
and July 2014. We estimated the average per-capita risk and expected
number of dengue cases for each host-city and each game schedule chosen based on
reported dengue cases to the Brazilian Ministry of Health for the period between
2010-2013. On the average, the expected number of cases among the 600,000 foreigner
tourists during the World Cup is 33, varying from 3-59. Such risk estimates will not
only benefit individual travellers for adequate pre-travel preparations, but also
provide valuable information for public health professionals and policy makers
worldwide. Furthermore, estimates of dengue cases in international travellers during
the World Cup can help to anticipate the theoretical risk for exportation of dengue
into currently non-infected areas. 相似文献