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1.
Raisa Turja Anna Soirinsuo Hélène Budzinski Marie Hélène Devier Kari K. Lehtonen 《Comparative biochemistry and physiology. Toxicology & pharmacology : CBP》2013,157(1):80-92
Baltic Sea blue mussels (Mytilus trossulus) were used as sentinel organisms to detect the biological effects of chemical contamination in the low salinity environment. Mussels naturally adapted to a salinity of ca. 6.0 PSU were caged for 30 days at four sites along an assumed pollution gradient (salinity ca. 4.5 PSU) in the vicinity of Finland's largest oil refinery and harbor Kilpilahti in the Gulf of Finland. Tissue concentrations and accumulation rates of especially organic contaminants (PAHs, PCBs and organotins) were clearly elevated at the innermost coastal stations near the harbor area. Biological effects of contaminant exposure on caged mussels were evaluated by measuring a suite of biomarkers including catalase, glutathione S-transferase, superoxide dismutase, glutathione reductase, lipid peroxidation, acetylcholinesterase activity and lysosomal membrane stability. Mussels transplanted near the harbor area were able to elevate their antioxidant defense in response to environmental contamination. Reduced morphometric condition index and soft tissue growth rate together with increased lipid peroxidation and low lysosomal membrane stability were also observed at the most contaminated site. The results suggest that caging of M. trossulus for four weeks at lower salinity is a feasible method for the detection of environmental pollution also in low salinity areas of the Baltic Sea. 相似文献
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Aumont V Larronde F Richard T Budzinski H Decendit A Deffieux G Krisa S Mérillon JM 《Journal of biotechnology》2004,109(3):287-294
The use of Vitis vinifera cells grown in a 2 l-stirred tank bioreactor for producing isotopically 13C-labeled phenolic substances is presented. Several culture parameters were optimized to achieve characteristics of growth and polyphenol metabolism similar to that recorded in shake flasks. Administration of [1-13C]L-phenylalanine (3 mM) to grape cell suspension cultures led to the production of 13C-labeled stilbenes (trans- and cis-piceids), catechins (catechin and epicatechin) and anthocyanins (delphinidin-, cyanidin-, petunidin-, peonidin- and malvidin-3-O-beta-glucosides). Incorporation of [1-13C]L-phenylalanine into polyphenols was measured by means of 13C satellites in the proton NMR spectrum and EA-IRMS. The enrichment of labeling obtained for all the compounds (between 40 and 65%) is sufficient to investigate their absorption and metabolism in humans. 相似文献
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Pugia MJ Jortani SA Basu M Sommer R Kuo HH Murphy S Williamson D Vranish J Boyle PJ Budzinski D Valdes R Basu SC 《Glycoconjugate journal》2007,24(1):5-15
Urinary trypsin inhibitors (uTi) suppress serine proteases during inflammation. After liberation from proinhibitors (P-alpha-I
and I-alpha-I) by the white blood cell (WBC) response, uTi readily pass through the kidneys into urine. A key uTi, bikunin,
is attached to O-linked and N-linked glycoconjugates. Recently, uTi inhibitors, called uristatins, were found to lack the
O-linked glycoconjugates. Monoclonal antibodies were produced using purified uristatin and screened for binding differences
to uristatin, bikunin, P-α-I, and I-α-I. Antibody-binding patterns were characterized using immunoaffinity binding onto protein-chip
surfaces and analysis by Surface Enhanced Laser Desorption/Ionization mass spectrometry (SELDI), using specimens from patients
and from purified uTi standards. Antibodies were developed and used in an enzyme-linked immunosorbent assay (ELISA) method
for uTi measurement in urine and plasma specimens. ELISA was performed on specimens from normal, presumed healthy, controls
and from patients who had been screened for inflammation using a high sensitivity C-reactive protein (CRP) test and a complete
blood count (CBC). Polyclonal antibody against uTi showed cross-reactivity with the Tamm–Horsfall protein (THP) and with proinhibitors.
Screening of anti-uTi monoclonal antibodies (Mab) revealed antibodies that did not cross-react with either of the above, thus
providing a tool to measure both uristatin and bikunin in urine with Mab 3G5 and in plasma with Mab 5D11. The monoclonal antibody
5D11 cross-reacts with specific N-linked glycoconjugates of uristatin present in plasma. In ca 96% of healthy adults, uTi
were present at <12 mg/l in urine and <4 mg/l in plasma. We also found that patients with an inflammation and a CRP of >2.0 mg/l
had higher urinary concentrations of uTi than the control population in every subject. Free uristatin and bikunin pass readily
into urine and are primarily bound to heavy chains that constitute the proinhibitor form in plasma. 相似文献
5.
O Geffard E His H Budzinski M Seaman P Garrigues 《Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie》2001,324(12):1149-1155
Embryos and larvae of bivalves are frequently used in marine ecotoxicology for the purpose of assessing seawater quality, because they are very sensitive to pollutants and provide rapid responses. Laboratory studies, however, cannot accurately simulate natural conditions. We conducted bivalve embryo-larval studies in situ at the marina of Arcachon (south-west French Atlantic coast), in order to assess 'biological quality' of the water. One experiment conducted in winter 1999 (temperatures of 10 degrees C) with embryos of the Mediterranean mussel, Mytilus galloprovincialis, has shown that such tests are practicable in winter at low temperatures. This study did not show any deterioration in 'biological quality' of the water. Four series of experiments were subsequently performed during summer 2000 (ambient water temperatures of 19 to 22.4 degrees C) with embryos of the Japanese oyster, Crassostrea gigas. The results show that the 'sea water biological quality' deteriorates from the port entrance towards its inner part. To our knowledge, this is the first investigation of the marine environment in which bivalve embryos have been used in situ. They are very suitable for this type of study, because bivalve embryos and larvae are more sensitive to pollutants than the adults, and also because they belong to euryhaline species and the embryos tolerate summer temperatures (both species) as well as winter temperatures (mussels), allowing biomonitoring to be conducted all over the year. 相似文献
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Analysis of DNA damage at the dinucleoside monophosphate level: application to the formamido lesion.
E E Budzinski A E Maccubbin M S Evans M Kulesz-Martin H C Box 《Radiation research》1992,132(3):288-295
The dinucleoside monophosphates d(TpG), d(TpC), and d(TpT) were X-irradiated in oxygenated solution. In each case the modification of the dinucleoside in which the thymine base is degraded to a formamido remnant was observed as a principal product. The hydrolysis of the phosphoester bond of formamido-modified dinucleosides is much slower than that of the corresponding unmodified dinucleosides. This effect is also observable in the hydrolysis of irradiated DNA, where hydrolysis by nuclease P1 (plus acid phosphatase) generates the modified dinucleosides d(TFpN), TF being the modified thymidine. The total yield of the formamido lesion in all its forms, d(TFpN), exceeds the yield of any other base modification. 相似文献
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The electrons trapped in single crystals of rhamnose X-irradiated at low temperature were studied by ENDOR spectroscopy. Hyperfine couplings of protons in the environs of the electron have been determined from ENDOR measurements, including those of some of the more remote carbon-bound hydrogen atoms. The likely site of electron trapping in the crystal structure of rhamnose was inferred from calculations of the electric potential generated by the dipoles of hydroxy groups about preexisting void spaces. Electron-proton distances for nonexchangeable hydrogen atoms from points within the void were calculated from the crystal structure and compared with distances obtained from hyperfine couplings. Good agreement was obtained between experimental and calculated values. 相似文献
9.
Maria Carolina Quecine Thiago Falda Leite Andressa Peres Bini Thais Regiani Lívia Maria Franceschini Ilara Gabriela Frasson Budzinski Felipe Garbelini Marques M?nica Teresa Veneziano Labate Simone Guidetti-Gonzalez David Henry Moon Carlos Alberto Labate 《PloS one》2016,11(1)
Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredospores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies. 相似文献
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Electrons are trapped in intermolecular voids in single crystals of dulcitol X-irradiated at low temperature. The hyperfine interactions between the trapped electron and the protons of the hydroxy groups which form the trap suggest a highly symmetrical arrangement of two hydroxy groups in apposition. On the basis of this consideration the site in the crystal structure where trapping occurs was identified. In a parallel approach the crystal structure of dulcitol was surveyed for a suitable void encompassing a positive potential. The latter approach confirmed the same site in the crystal structure as the electron trapping site. 相似文献