Parametric studies on bioengineering effects of tree root-based suction on ground behaviour

Using native vegetation to improve soil stiffness, stabilise slopes and control erosion is a rapidly evolving process. A theoretical model previously developed by the authors for the rate of tree root water uptake together with an associated numerical simulation is used to study the effects of a wide range of soil, tree, and atmospheric parameters on partially saturated ground. The influence of different parameters on the maximum initial rate of root water uptake is investigated through parametric and sensitivity analyses. Field measurements taken from previously published literature are compared with numerical predictions for validation. The rate of selected parameters such as potential transpiration and its distribution, suction at wilting point, the coefficient of permeability and the distribution of root length density are studied in detail. The analysis shows that the rate of potential transpiration increases the soil matric suction and ground settlement, while the potential transpiration rate has an insignificant effect on the distribution of soil suction. Root density distribution factors affect the size of the influence zone. Suction at the wilting point increases the soil matric suction and ground settlement, whereas the saturation permeability decreases the maximum soil matric suction generated. The analysis confirms that the most sensitive parameters, including the coefficients of the tree root system, the transpiration rate, the permeability of the soil and its suction at the wilting point should be measured or estimated accurately for an acceptable prediction of ground conditions in the vicinity of trees.     

Preparation,Purification, and Characterization of Lanthanide Complexes for Use as Contrast Agents for Magnetic Resonance Imaging

Polyaminopolycarboxylate-based ligands are commonly used to chelate lanthanide ions, and the resulting complexes are useful as contrast agents for magnetic resonance imaging (MRI). Many commercially available ligands are especially useful because they contain functional groups that allow for fast, high-purity, and high-yielding conjugation to macromolecules and biomolecules via amine-reactive activated esters and isothiocyanate groups or thiol-reactive maleimides. While metalation of these ligands is considered common knowledge in the field of bioconjugation chemistry, subtle differences in metalation procedures must be taken into account when selecting metal starting materials. Furthermore, multiple options for purification and characterization exist, and selection of the most effective procedure partially depends on the selection of starting materials. These subtle differences are often neglected in published protocols. Here, our goal is to demonstrate common methods for metalation, purification, and characterization of lanthanide complexes that can be used as contrast agents for MRI (Figure 1). We expect that this publication will enable biomedical scientists to incorporate lanthanide complexation reactions into their repertoire of commonly used reactions by easing the selection of starting materials and purification methods.     

Bioengineering ground improvement considering root water uptake model

Bioengineering features of native vegetation are currently being evolved to enhance soil stiffness, slope stabilisation and erosion control. The effects of tree roots on soil moisture content and ground settlement are discussed in this paper. Matric suction induced by tree roots is a key factor, governing the properties of unsaturated soils, directly imparting stability to slopes and resistance for yielding behaviour. A mathematical model for the rate of root water uptake that considers ground conditions, type of vegetation and climatic parameters has been developed. This study highlights the inter-related parameters contributing to the development of a conceptual evapo-transpiration and root moisture uptake equilibrium model that is then incorporated in a comprehensive numerical finite element model. The developed model considers fully coupled-flow-deformation behaviour of soil. Field measurements obtained by the Authors from a site in Victoria, South of Australia, are used to validate the model. In this study, the active tree root distribution has been predicted by measuring soil organic content distribution. The predicted results show acceptable agreement with the field data in spite of the assumptions made for simplifying the effects of soil heterogeneity and anisotropy. The results prove that the proposed root water uptake model can reliably predict the region of the maximum matric suction away from the tree axis.     

A proposal for identifying the low renal uric acid clearance phenotype

Investigation of the genetic basis of hyperuricaemia is a subject of intense interest. However, clinical studies commonly include hyperuricaemic patients without distinguishing between 'over-producers' or 'under-excretors' of urate. The statistical power of studies of genetic polymorphisms of genes encoding renal urate transporters is diluted if 'over-producers' of uric acid are included. We propose that lower than normal fractional renal clearance of urate is a better inclusion criterion for these studies. We also propose that a single daytime spot urine sample for calculation of fractional renal clearance of urate should be preferred to calculation from 24-hour urine collections.     

Fractional clearance of urate: validation of measurement in spot-urine samples in healthy subjects and gouty patients

Introduction

Hyperuricemia is the greatest risk factor for gout and is caused by an overproduction and/or inefficient renal clearance of urate. The fractional renal clearance of urate (FCU, renal clearance of urate/renal clearance of creatinine) has been proposed as a tool to identify subjects who manifest inefficient clearance of urate. The aim of the present studies was to validate the measurement of FCU by using spot-urine samples as a reliable indicator of the efficiency of the kidney to remove urate and to explore its distribution in healthy subjects and gouty patients.

Methods

Timed (spot, 2-hour, 4-hour, 6-hour, 12-hour, and 24-hour) urine collections were used to derive FCU in 12 healthy subjects. FCUs from spot-urine samples were then determined in 13 healthy subjects twice a day, repeated on 3 nonconsecutive days. The effect of allopurinol, probenecid, and the combination on FCU was explored in 11 healthy subjects. FCU was determined in 36 patients with gout being treated with allopurinol. The distribution of FCU was examined in 118 healthy subjects and compared with that from the 36 patients with gout.

Results

No substantive or statistically significant differences were observed between the FCUs derived from spot and 24-hour urine collections. Coefficients of variation (CVs) were both 28%. No significant variation in the spot FCU was obtained either within or between days, with mean intrasubject CV of 16.4%. FCU increased with probenecid (P < 0.05), whereas allopurinol did not change the FCU in healthy or gouty subjects. FCUs of patients with gout were lower than the FCUs of healthy subjects (4.8% versus 6.9%; P < 0.0001).

Conclusions

The present studies indicate that the spot-FCU is a convenient, valid, and reliable indicator of the efficiency of the kidney in removing urate from the blood and thus from tissues. Spot-FCU determinations may provide useful correlates in studies investigating molecular mechanisms underpinning the observed range of efficiencies of the kidneys in clearing urate from the blood.

Trial Registration

ACTRN12611000743965