全文获取类型
收费全文 | 281篇 |
免费 | 29篇 |
专业分类
310篇 |
出版年
2021年 | 5篇 |
2020年 | 2篇 |
2018年 | 4篇 |
2017年 | 5篇 |
2016年 | 5篇 |
2015年 | 9篇 |
2014年 | 7篇 |
2013年 | 8篇 |
2012年 | 11篇 |
2011年 | 11篇 |
2010年 | 12篇 |
2009年 | 8篇 |
2008年 | 8篇 |
2007年 | 12篇 |
2006年 | 10篇 |
2005年 | 11篇 |
2004年 | 22篇 |
2003年 | 11篇 |
2002年 | 15篇 |
2001年 | 17篇 |
2000年 | 12篇 |
1999年 | 17篇 |
1998年 | 5篇 |
1997年 | 4篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1993年 | 2篇 |
1992年 | 7篇 |
1991年 | 8篇 |
1988年 | 5篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1979年 | 3篇 |
1978年 | 2篇 |
1976年 | 2篇 |
1961年 | 2篇 |
1957年 | 3篇 |
1956年 | 2篇 |
1955年 | 2篇 |
1954年 | 2篇 |
1953年 | 1篇 |
1952年 | 1篇 |
1949年 | 1篇 |
1942年 | 1篇 |
1940年 | 2篇 |
1937年 | 2篇 |
1935年 | 2篇 |
1933年 | 1篇 |
1926年 | 1篇 |
1925年 | 1篇 |
排序方式: 共有310条查询结果,搜索用时 0 毫秒
1.
Buchner DM 《The Western journal of medicine》1988,148(2):198-199
2.
3.
4.
5.
Alternatively folded states of an immunoglobulin 总被引:1,自引:0,他引:1
J Buchner M Renner H Lilie H J Hinz R Jaenicke T Kiefhabel R Rudolph 《Biochemistry》1991,30(28):6922-6929
Well-defined, non-native protein structures of low stability have been increasingly observed as intermediates in protein folding or as equilibrium structures populated under specific solvent conditions. These intermediate structures, frequently referred to as molten globule states, are characterized by the presence of secondary structure, a lack of significant tertiary contacts, increased hydrophobicity and partial specific volume as compared to native structures, and low cooperativity in thermal unfolding. The present study demonstrates that under acidic conditions (pH less than 3) the antibody MAK33 can assume a folded stable conformation. This A-state is characterized by a high degree of secondary structure, increased hydrophobicity, a native-like maximum wavelength of fluorescence emission, and a tendency toward slow aggregation. A prominent feature of this low-pH conformation is the stability against denaturant and thermal unfolding that is manifested in highly cooperative reversible phase transitions indicative of the existence of well-defined tertiary contacts. These thermodynamic results are corroborated by the kinetics of folding from the completely unfolded chain to the alternatively folded state at pH 2. The given data suggest that MAK33 at pH 2 adopts a cooperative structure that differs from the native immunoglobulin fold at pH 7. This alternatively folded state exhibits certain characteristics of the molten globule but differs distinctly from it by its extraordinary structural stability that is characteristic for native protein structures. 相似文献
6.
7.
8.
BiPPred: Combined sequence‐ and structure‐based prediction of peptide binding to the Hsp70 chaperone BiP 下载免费PDF全文
Manuel Glaser Atanas Patronov Harpreet Shah Katrin Christiane Back Marina Angelika Daake Johannes Buchner Iris Antes 《Proteins》2016,84(10):1390-1407
Substrate binding to Hsp70 chaperones is involved in many biological processes, and the identification of potential substrates is important for a comprehensive understanding of these events. We present a multi‐scale pipeline for an accurate, yet efficient prediction of peptides binding to the Hsp70 chaperone BiP by combining sequence‐based prediction with molecular docking and MMPBSA calculations. First, we measured the binding of 15mer peptides from known substrate proteins of BiP by peptide array (PA) experiments and performed an accuracy assessment of the PA data by fluorescence anisotropy studies. Several sequence‐based prediction models were fitted using this and other peptide binding data. A structure‐based position‐specific scoring matrix (SB‐PSSM) derived solely from structural modeling data forms the core of all models. The matrix elements are based on a combination of binding energy estimations, molecular dynamics simulations, and analysis of the BiP binding site, which led to new insights into the peptide binding specificities of the chaperone. Using this SB‐PSSM, peptide binders could be predicted with high selectivity even without training of the model on experimental data. Additional training further increased the prediction accuracies. Subsequent molecular docking (DynaDock) and MMGBSA/MMPBSA‐based binding affinity estimations for predicted binders allowed the identification of the correct binding mode of the peptides as well as the calculation of nearly quantitative binding affinities. The general concept behind the developed multi‐scale pipeline can readily be applied to other protein‐peptide complexes with linearly bound peptides, for which sufficient experimental binding data for the training of classical sequence‐based prediction models is not available. Proteins 2016; 84:1390–1407. © 2016 Wiley Periodicals, Inc. 相似文献
9.
10.
Jona Kayser Martin Haslbeck Lisa Dempfle Maike Krause Carsten Grashoff Johannes Buchner Harald Herrmann Andreas?R. Bausch 《Biophysical journal》2013,105(8):1778-1785
The mechanical properties of living cells are essential for many processes. They are defined by the cytoskeleton, a composite network of protein fibers. Thus, the precise control of its architecture is of paramount importance. Our knowledge about the molecular and physical mechanisms defining the network structure remains scarce, especially for the intermediate filament cytoskeleton. Here, we investigate the effect of small heat shock proteins on the keratin 8/18 intermediate filament cytoskeleton using a well-controlled model system of reconstituted keratin networks. We demonstrate that Hsp27 severely alters the structure of such networks by changing their assembly dynamics. Furthermore, the C-terminal tail domain of keratin 8 is shown to be essential for this effect. Combining results from fluorescence and electron microscopy with data from analytical ultracentrifugation reveals the crucial role of kinetic trapping in keratin network formation. 相似文献