The portal vein and its main affluents. An anatomic and morphometric study

There are reported the results of certain measurements made on 158 human cadavers of both sexes. A series of main parameters are given, such as the distance between the superior mesenteric vein and on the one hand its confluence with other vessels and on the other hand the pancreas and the horizontal part of the duodenum: the distance between the superior mesenteric vein together with the portal vein, and the inferior caval vein; the diameters of the portal trunk and the confluence angle between the roots of the portal vein. The results reflect the possibility to perform a troncular portocaval anastomosis in at least 90 per cent of the cases. The authors' attention was, however, directed towards the anatomy of the superior mesenteric vein, as this vessel is preferred by some surgeons in the achievement of the portocaval anastomosis.     

An integrative expression vector for <Emphasis Type="Italic">Actinosynnema pretiosum</Emphasis>

Background  

The Actinomycete Actinosynnema pretiosum ssp. auranticum has commercial importance due to its production of ansamitocin P-3 (AP-3), a potent antitumor agent. One way to increase AP-3 production would be to constitutively express selected genes so as to relieve bottlenecks in the biosynthetic pathway; however, an integrative expression vector for A. pretiosum is lacking. The aim of this study was to construct a vector for heterologous gene expression in A. pretiosum.     

Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics

BACKGROUND: Quantitative proteomics is an emerging field that encompasses multiplexed measurement of many known proteins in groups of experimental samples in order to identify differences between groups. Antibody arrays are a novel technology that is increasingly being used for quantitative proteomics studies due to highly multiplexed content, scalability, matrix flexibility and economy of sample consumption. Key applications of antibody arrays in quantitative proteomics studies are identification of novel diagnostic assays, biomarker discovery in trials of new drugs, and validation of qualitative proteomics discoveries. These applications require performance benchmarking, standardization and specification. RESULTS: Six dual-antibody, sandwich immunoassay arrays that measure 170 serum or plasma proteins were developed and experimental procedures refined in more than thirty quantitative proteomics studies. This report provides detailed information and specification for manufacture, qualification, assay automation, performance, assay validation and data processing for antibody arrays in large scale quantitative proteomics studies. CONCLUSION: The present report describes development of first generation standards for antibody arrays in quantitative proteomics. Specifically, it describes the requirements of a comprehensive validation program to identify and minimize antibody cross reaction under highly multiplexed conditions; provides the rationale for the application of standardized statistical approaches to manage the data output of highly replicated assays; defines design requirements for controls to normalize sample replicate measurements; emphasizes the importance of stringent quality control testing of reagents and antibody microarrays; recommends the use of real-time monitors to evaluate sensitivity, dynamic range and platform precision; and presents survey procedures to reveal the significance of biomarker findings.     

First survey on hard ticks (Ixodidae) collected from humans in Romania: possible risks for tick-borne diseases

The importance of studies on the diversity of ticks attacking humans resides mostly in the relatively highly-specific tick-pathogen associations. Human tick bites are commonly reported worldwide but removal of ticks from patients is rarely followed by specific identification of the ticks, leaving to some degree of hazard the preventive treatment of possible associated diseases. A total number of 308 ticks were collected between April and June 2010 from 275 human patients who voluntarily presented to a hospital from Cluj-Napoca, Romania. The mean intensity of infection was 1.12 ± 0.46. Four species of ticks were identified Ixodes ricinus, Dermacentor marginatus, Haemaphysalis concinna and H. punctata. Ixodes ricinus was the most abundant species feeding on humans in the study area. A brief review of possible associated pathogen is provided.     

The Influence of Urbanism and Information Consumption on Political Dimensions of Social Capital: Exploratory Study of the Localities Adjacent to the Core City from Bra?ov Metropolitan Area,Romania

Background

The last two decades have seen a growing trend towards the research of voting behavior in post-communist countries. Urban sociology theorists state that not only space structures influence political participation, but also space structures are changing under the influence of global, local, and individual factors. The growing role played by information in the globalised world has accelerated the paradigm shift in urban sociology: from central place model (based on urban-rural distinction and on monocentric metropolitan areas) to network society (based on space of flows and polycentric metropolitan areas). However, recent studies have mainly focused on countries with solid democracies, rather than on former communist countries. The present study aims to analyze the extent to which a new emerging spatial structure can be envisaged within a metropolitan area of Romania and its consequences for the political dimensions of social capital.

Methods

The Transilvania University Ethics Commission approved this study (S1 Aprouval). The research is based upon individual and aggregate empirical data, collected from the areas adjacent to the core city in Brașov metropolitan area. Individual data has been collected during October 2012, using the oral survey technique (S1 Survey), based on a standardized questionnaire (stratified simple random sample, N = 600). The National Institute of Statistics and the Electoral Register provided the aggregate data per locality. Unvaried and multivariate analyses (hierarchical regression method) were conducted based on these data.

Results

Some dimensions of urbanism, identified as predictors of the political dimensions of social capital, suggest that the area under analysis has a predominantly monocentric character, where the rural-urban distinction continues to remain relevant. There are also arguments favoring the dissolution of the rural-urban distinction and the emergence of polycentric spatial structures. The presence of some influences related to the information consumption on all six indicators of the political dimensions of social capital under analysis suggests the occurrence of emerging forms of a space of flows. The identified effects of social problems associated with transport infrastructure and of migration experience on the political dimensions of social capital, also support the emergence of space of flows.

Conclusions

We recommend that, in the urban studies in former communist countries, conceptualization of urbanism as predictor of the political dimensions of social capital should consider both the material dimensions of space, as well as the dimensions of information consumption and migration experience.     

The CD11a partner in Sus scrofa lymphocyte function-associated antigen-1 (LFA-1): mRNA cloning, structure analysis and comparison with mammalian homologues

Background  

Lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alphaLbeta2), the most abundant and widely expressed beta2-integrin, is required for many cellular adhesive interactions during the immune response. Many studies have shown that LFA-1 is centrally involved in the pathogenesis of several diseases caused by Repeats-in-toxin (RTX) -producing bacteria.