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排序方式: 共有79条查询结果,搜索用时 15 毫秒
1.
The vitamin D binding protein (Gc) and posttransferrin-2 (Ptf-2) phenotypes have been determined in a number of Belgian cattle breeds. A very slow migrating variant of the Gc protein — Gc C — has been found in White and Red East Flemish breed. This variant was absent from the other breeds studied. This slow variant was identified as a vitamin D binding protein by autoradiography. The Gc C protein was shown to be controlled by a codominant autosomal allele Gc C at the Gclocus. The Gc C protein is probably identical with a fraction previously described in buffalo and an Italian cattle breed. The allele frequencies for the Gc and Pft-2 systems are reported for several Belgian breeds of cattle. 相似文献
2.
The linkage of the Phi, Pgd, Po2, S, H and halothane sensitivity loci was followed in a Belgian Landrace family, heterozygous for these systems over 6 generations. Recombination next to the S locus occurred mainly in pigs belonging to this particular family. From this investigation the position of the S locus is proved to be outwith the Phi-Pgd region, next to Phi . Therefore the gene sequence S - Phi - Hal -H- Po2 -Pgd is proposed. Higher recombination rates were observed in the female parental line of the multiheterozygous family when compared to the male parental line. Additional data from animals, unrelated to this strain, confirm the evidence of close linkage of the S system to the nearest marker loci. 相似文献
3.
Newcastle disease virus fusion protein expressed in a fowlpox virus recombinant confers protection in chickens. 总被引:23,自引:2,他引:21
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J Taylor C Edbauer A Rey-Senelonge J F Bouquet E Norton S Goebel P Desmettre E Paoletti 《Journal of virology》1990,64(4):1441-1450
A cDNA copy of the RNA encoding the fusion (F) protein of Newcastle disease virus (NDV) strain Texas, a velogenic strain of NDV, was obtained and the sequence was determined. The 1,792-base-pair sequence encodes a protein of 553 amino acids which has essential features previously established for the F protein of virulent NDV strains. These include the presence of three strongly hydrophobic regions and pairs of dibasic amino acids in the pentapeptide Arg-Arg-Gln-Arg-Arg preceding the putative cleavage site. When inserted into a fowlpox virus vector, a glycosylated protein was expressed and presented on the surface of infected chicken embryo fibroblast cells. The F protein expressed by the recombinant fowlpox virus was cleaved into two polypeptides. When inoculated into susceptible birds by a variety of routes, an immunological response was induced. Ocular or oral administration of the recombinant fowlpox virus gave partial protection, whereas both intramuscular and wing-web routes of inoculation gave complete protection after a single inoculation. 相似文献
4.
High levels of regeneration were obtained from young leaves excised from axillary shoots in proliferating nodal cultures of several Vitis x Muscadinia hybrids. Best results were obtained when the explants were cultured on solidified half-strength Murashige and Skoog medium supplemented with 8.9 M 6-benzyladenine and 0.05 M 1-naphthaleneacetic acid. Though variation was observed among the hybrids, the procedure used does not seem to be genotype-specific as all the hybrids and cultivars tested could regenerate. Scanning electron microscopy observations and histological studies carried out during the development of adventitious shoot organogenesis revealed that the promeristem initiation occurred in the outer cell layers near the wounded area of the petiolar stub.Abbreviations BA
6-benzyladenine
- NAA
1-naphthaleneacetic acid 相似文献
5.
A female twin was born after non-surgical transfer of one 7 day old embryo to the uterus of a synchronized receptor heifer. To prove the monozygocity of the twin, the hemotype was analysed (10 blood group systems and the polymorphic blood substances). The complete hemotype was identical in both twin calves. The probability that an identical genotype in a dizygotic twin could occur only by chance was calculated to be 23.8 x 10(-8). The bisectioning of the embryo most probably occurred during hatching at about day 8. 相似文献
6.
7.
Anne-Christine Hick Anne-Sophie Delmarcelle Mahé Bouquet Sabrina Klotz Tamara Copetti Celine Forez Patrick Van Der Smissen Pierre Sonveaux Jean-François Collet Olivier Feron Pierre J. Courtoy Christophe E. Pierreux 《Developmental biology》2013
The thyroid is a highly vascularized endocrine gland, displaying a characteristic epithelial organization in closed spheres, called follicles. Here we investigate how endothelial cells are recruited into the developing thyroid and if they control glandular organization as well as thyrocytes and C-cells differentiation. We show that endothelial cells closely surround, and then invade the expanding thyroid epithelial cell mass to become closely associated with nascent polarized follicles. This close and sustained endothelial:epithelial interaction depends on epithelial production of the angiogenic factor, Vascular Endothelial Growth Factor-A (VEGF-A), as its thyroid-specific genetic inactivation reduced the endothelial cell pool of the thyroid by >90%. Vegfa KO also displayed decreased C-cells differentiation and impaired organization of the epithelial cell mass into follicles. We developed an ex vivo model of thyroid explants that faithfully mimicks bilobation of the thyroid anlagen, endothelial and C-cells invasion, folliculogenesis and differentiation. Treatment of thyroid explants at e12.5 with a VEGFR2 inhibitor ablated the endothelial pool and reproduced ex vivo folliculogenesis defects observed in conditional Vegfa KO. In the absence of any blood supply, rescue by embryonic endothelial progenitor cells restored folliculogenesis, accelerated lumen expansion and stimulated calcitonin expression by C-cells. In conclusion, our data demonstrate that, in developing mouse thyroid, epithelial production of VEGF-A is necessary for endothelial cells recruitment and expansion. In turn, endothelial cells control epithelial reorganization in follicles and C-cells differentiation. 相似文献
8.
Angela Feechan Claire Anderson Laurent Torregrosa Angelica Jermakow Pere Mestre Sabine Wiedemann‐Merdinoglu Didier Merdinoglu Amanda R. Walker Lance Cadle‐Davidson Bruce Reisch Sebastien Aubourg Nadia Bentahar Bipna Shrestha Alain Bouquet Anne‐Françoise Adam‐Blondon Mark R. Thomas Ian B. Dry 《The Plant journal : for cell and molecular biology》2013,76(4):661-674
The most economically important diseases of grapevine cultivation worldwide are caused by the fungal pathogen powdery mildew (Erysiphe necator syn. Uncinula necator) and the oomycete pathogen downy mildew (Plasmopara viticola). Currently, grapegrowers rely heavily on the use of agrochemicals to minimize the potentially devastating impact of these pathogens on grape yield and quality. The wild North American grapevine species Muscadinia rotundifolia was recognized as early as 1889 to be resistant to both powdery and downy mildew. We have now mapped resistance to these two mildew pathogens in M. rotundifolia to a single locus on chromosome 12 that contains a family of seven TIR‐NB‐LRR genes. We further demonstrate that two highly homologous (86% amino acid identity) members of this gene family confer strong resistance to these unrelated pathogens following genetic transformation into susceptible Vitis vinifera winegrape cultivars. These two genes, designated r esistance to P lasmopara v iticola (MrRPV1) are the first resistance genes to be cloned from a grapevine species. Both MrRUN1 and MrRPV1 were found to confer resistance to multiple powdery and downy mildew isolates from France, North America and Australia; however, a single powdery mildew isolate collected from the south‐eastern region of North America, to which M. rotundifolia is native, was capable of breaking MrRUN1‐mediated resistance. Comparisons of gene organization and coding sequences between M. rotundifolia and the cultivated grapevine V. vinifera at the MrRUN1/MrRPV1 locus revealed a high level of synteny, suggesting that the TIR‐NB‐LRR genes at this locus share a common ancestor. 相似文献
9.
Identification of resistance gene analogs linked to a powdery mildew resistance locus in grapevine 总被引:18,自引:6,他引:12
Donald TM Pellerone F Adam-Blondon AF Bouquet A Thomas MR Dry IB 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):610-618
Oligonucleotide primers, designed to conserved regions of nucleotide binding site (NBS) motifs within previously cloned pathogen
resistance genes, were used to amplify resistance gene analogs (RGAs) from grapevine. Twenty eight unique grapevine RGA sequences
were identified and subdivided into 22 groups on the basis of nucleic acid sequence-identity of approximately 70% or greater.
Representatives from each group were used in a bulked segregant analysis strategy to screen for restriction fragment length
polymorphisms linked to the powdery mildew resistance locus, Run1, introgressed into Vitis vinifera L. from the wild grape species Muscadinia rotundifolia. Three RGA markers were found to be tightly linked to the Run1 locus. Of these markers, two (GLP1–12 and MHD145) cosegregated with the resistance phenotype in 167 progeny tested, whereas
the third marker (MHD98) was mapped to a position 2.4 cM from the Run1 locus. The results demonstrate the usefulness of RGA sequences, when used in combination with bulked segregant analysis,
to rapidly generate markers tightly linked to resistance loci in crop species.
Received: 2 May 2001 / Accepted: 3 August 2001 相似文献
10.
Identification of a codominant scar marker linked to the seedlessness character in grapevine 总被引:21,自引:0,他引:21
F. Lahogue P. This A. Bouquet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):950-959
The variety Vitis vinifera cv Sultanine presents a type of seedlessness in which fertilization occurs but seeds subsequently fail to develop. It has
been suggested that this trait might be controlled by three complementary recessive genes regulated by a dominant gene named
I. Bulk segregant analysis was used to search for random amplified polymorphic DNA (RAPD) markers linked to the I gene in progeny obtained by crossing two partially seedless genotypes. One hundred and forty decamer primers were screened
using bulks obtained by pooling the DNA of extreme individuals from the phenotypic distribution. We identified two RAPD markers
which appeared tightly linked to I (at 0.7 and 3.5 cM respectively). The closest marker was used to develop a codominant SCAR (sequence characterized amplified
region), named SCC8. This latter marker appeared of great value either to exclude from the progeny potentially seeded individuals or to select
for seedless individuals. Indeed, all the seeded individuals of the progeny were found to be homozygous scc8
-/scc8
-, and all the individuals homozygous SCC8
+/SCC8
+ were seedless. Moreover, this marker was successfully applied to other natural seedless varieties where codominance persisted.
SCC8 was also used to dissect more precisely the genetics of seedlessness. ANOVA analysis indicated that this SCAR marker accounted
for at least 64.9% of the phenotypic variation of the seed’s fresh weight and for at least 78.7% of the phenotypic variation
of the seed’s dry matter. These results confirmed the presence of a major gene, and also the existence of other complementary
recessive genes, controlling the expression of seedlessness.
Received: 29 July 1997 / Accepted: 16 March 1998 相似文献