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Mariem Khouja Rita C. Alves Diana Melo Anabela S. G. Costa M. Antonia Nunes Abdelhamid Khaldi M. Beatriz P. P. Oliveira Chokri Messaoud 《化学与生物多样性》2021,18(6):e2100071
The lipid fraction of seeds from different pine species and populations was studied regarding total lipid content, fatty acid profile and vitamin E composition. The investigated seeds contained a high percentage of lipid (13.6 to 31.5 %). Lipid fractions were found to be rich in vitamin E, which varied significantly among species and populations. P. halepensis (Ph−Hn) showed the highest content of vitamin E (256.3 mg/kg of seeds) and the uppermost content of α-tocopherol (44 mg/kg). However, P. halepensis (Ph−Kas) was the richest in γ-tocopherol (204.9 mg/kg). Lipid fractions had a low content of δ-tocopherol (1.2 to 3.6 mg/kg. The highest content of γ-tocotrienol (∼18 %) was determined for P. halepensis (Ph−Dc and Ph−Hn). Thirteen fatty acids were identified by GC-FID with significant variation between the investigated species. The linoleic acid was the major fatty acid followed by oleic acid and palmitic acid. The chemical differentiation among species for the composition of fatty acids and vitamin E was confirmed by PCA. Significant correlations were observed between the content of vitamin E and fatty acids and ecological parameters of P. halepensis populations. 相似文献
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Sahli C. Bahri W. Fredj S. H. Dabboubi R. Bousseta K. Mehrzi A. Messaoud T. 《Russian Journal of Genetics》2019,55(6):756-761
Russian Journal of Genetics - The aim of this study was to determine a possible association of IVS3 poly A and IVS10 CA microsatellites with CF in case–control Tunisian groups and to compare... 相似文献
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Flihi Jihène Rhimi Awatef Yangui Islem Messaoud Chokri Ben ElHadj Ali Imen 《Molecular biology reports》2022,49(7):6215-6224
Molecular Biology Reports - In Tunisia, Kermes oak (Quercus coccifera L.) populations are severely destroyed due to deforestation. Nowadays, no preservation programs are attempted, yet, to conserve... 相似文献
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Extracts of mature dark blue and white berries from two Tunisian Myrtus communis morphs growing at the same site were assessed for their essential‐oil and fatty‐acid compositions, phenolic contents, and antioxidant activities. The GC and GC/MS analyses of the essential oils allowed the identification of 33 constituents. The oils from the dark blue fruits showed high percentages of α‐pinene (11.1%), linalool (11.6%), α‐terpineol (15.7%), methyl eugenol (6.2%), and geraniol (3.7%). Myrtenyl acetate (20.3%) was found to be the major compound in the oils from white berries. GC Analysis of the pericarp and seed fatty acids showed that the polyunsaturated fatty acids constituted the major fraction (54.3–78.1%). The highest percentages of linoleic acid (78.0%) and oleic acid (20.0%) were observed in the seeds and the pericarps of the white fruits, respectively. The total phenol, flavonoid, and flavonol contents and the concentration of the eight anthocyanins, identified by HPLC analysis, were significantly higher in the dark blue fruits. All extracts showed a substantial antioxidant activity, assessed by the free radical‐scavenging activity and the ferric reducing power, with the dark blue fruit extracts being more effective. 相似文献
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Guillaume Gaullier Simona Miron Sabrina Pisano Rémi Buisson Yann-Va? Le?Bihan Carine Tellier-Lebègue Wala Messaoud Pierre Roblin Beatriz G. Guimar?es Robert Thai Marie-Josèphe Giraud-Panis Eric Gilson Marie-Hélène Le?Du 《Nucleic acids research》2016,44(4):1962-1976
Telomere integrity is essential to maintain genome stability, and telomeric dysfunctions are associated with cancer and aging pathologies. In human, the shelterin complex binds TTAGGG DNA repeats and provides capping to chromosome ends. Within shelterin, RAP1 is recruited through its interaction with TRF2, and TRF2 is required for telomere protection through a network of nucleic acid and protein interactions. RAP1 is one of the most conserved shelterin proteins although one unresolved question is how its interaction may influence TRF2 properties and regulate its capacity to bind multiple proteins. Through a combination of biochemical, biophysical and structural approaches, we unveiled a unique mode of assembly between RAP1 and TRF2. The complete interaction scheme between the full-length proteins involves a complex biphasic interaction of RAP1 that directly affects the binding properties of the assembly. These results reveal how a non-DNA binding protein can influence the properties of a DNA-binding partner by mutual conformational adjustments. 相似文献
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Mabrouk SB Messaoud EB Ayadi D Jemli S Roy A Mezghani M Bejar S 《Molecular biotechnology》2008,38(3):211-219
A gene encoding maltogenic amylase from acidic Bacillus sp. US149 (maUS149) was cloned, sequenced and over-expressed in Escherichia coli. The nucleotide sequence analysis revealed an open reading frame (ORF) of 1749 bp encoding a protein of 582 residues. The
alignment of deduced amino acid sequence revealed a relatively low homology with the already reported maltogenic amylases.
In fact, its highest identity, of only 60%, was found with the maltogenic amylase of Thermus sp. IM6501. The recombinant enzyme (MAUS149) was found to be intracellular and was purified to homogeneity from the cell
crude extract with a yield of 23%. According to PAGE analysis, under reducing and non-reducing conditions, the recombinant
enzyme has an apparent molecular weight of 135 kDa and is composed of two identical subunits of 67.5 kDa each. The maximum
activity was obtained at 40°C and pH 6.5. MAUS149 could be classified as a maltogenic amylase since it produces mainly maltose
from starch, maltose and glucose from β-cyclodextrin, and panose from pullulan. 相似文献
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Ghada Baraket Khaled Chatti Olfa Saddoud Ahmed Ben Abdelkarim Messaoud Mars Mokhtar Trifi Amel Salhi Hannachi 《Plant Molecular Biology Reporter》2011,29(1):171-184
This study characterises the genetic variability of fig, Ficus carica L., using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. It compares the efficiency
and utility of the two techniques in detecting variation and establishing genetic relationships among Tunisian fig cultivars.
Our results show that using both marker systems, the Tunisian fig germ plasm is characterised by having a large genetic diversity
at the deoxyribonucleic acid level, as most of AFLP bands were detected and all SSR markers were polymorphic. In fact, 351
(342 polymorphic) and 57 (57 polymorphic) bands were detected using AFLP and SSR primers, respectively. SSR markers were the
most polymorphic with an average polymorphic information content value of 0.94, while AFLP markers showed the highest effective
multiplex ratio (56.9) and marker index (45.2). The effective marker index was recorded highest (4.19) for AFLP markers and
lowest (0.70) for the SSR ones. Our results demonstrate that (1) independent as well as combined analyses of cluster analyses
of SSR and AFLP fragments showed that cultivars are clustered independently from their geographical origin, horticultural
classifications and tree sex; (2) the analysis of molecular variance allowed the partitioning of genetic variation within
and among fig groups and showed greater variation within groups and (3) AFLP and SSR markers datasets showed positive correlation.
This study suggests the SSR and AFLP markers are suitable for diversity analysis and cultivars fingerprinting. An understanding
of the genetic diversity and population structure of F. carica in Tunisia can also provide insight into the conservation and management of this species. 相似文献
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Ping An Shinobu Inanaga Nanwen Zhu Xiangjun Li Hassan M. Fadul Messaoud Mars 《African Journal of Ecology》2007,45(1):94-102
We examined the distribution of plants in sandy rangelands with different degrees of desertification in (i) Horqin and Mu Us sandy lands in north China, (ii) Darfur district in north‐west Sudan and (iii) southern Tunisia. The severity of desertification in these areas was classified as light, moderate, severe or extreme. The following vegetation changes were observed with increasing severity of desertification: a decrease in the number of plant species, an increase in drought‐tolerant plants, a decrease in the proportion of palatable grasses and the occurrence of some species in only one category of desertification, whereas other species occurred in several categories. Plant species with unique niche requirements were identified and recommended as indicators of the degree of desertification for particular areas. We discuss the characteristics of species in areas with severe desertification and the method of using plant species as indicators of the extent of desertification. 相似文献