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1.
Genadii B Borovskii Irina V Stupnikova Anna I Antipina Svetlana V Vladimirova Victor K Voinikov 《BMC plant biology》2002,2(1):5-7
Background
Dehydrins are known as Group II late embryogenesis abundant proteins. Their high hydrophilicity and thermostability suggest that they may be structure stabilizers with detergent and chaperone-like properties. They are localised in the nucleus, cytoplasm, and plasma membrane. We have recently found putative dehydrins in the mitochondria of some cereals in response to cold. It is not known whether dehydrin-like proteins accumulate in plant mitochondria in response to stimuli other than cold stress.Results
We have found five putative dehydrins in the mitochondria of winter wheat, rye and maize seedlings. Two of these polypeptides had the same molecular masses in all three species (63 and 52 kD) and were thermostable. Drought, freezing, cold, and exogenous ABA treatment led to higher accumulation of dehydrin-like protein (dlp) 63 kD in the rye and wheat mitochondria. Protein 52 kD was induced by cold adaptation and ABA. Some accumulation of these proteins in the maize mitochondria was found after cold exposition only. The other three proteins appeared to be heat-sensitive and were either slightly induced or not induced at all by all treatments used.Conclusions
We have found that, not only cold, but also drought, freezing and exogenous ABA treatment result in accumulation of the thermostable dehydrins in plant mitochondria. Most cryotolerant species such as wheat and rye accumulate more heat-stable dehydrins than cryosensitive species such as maize. It has been supposed that their function is to stabilize proteins in the membrane or in the matrix. Heat-sensitive putative dehydrins probably are not involved in the stress reaction and adaptation of plants. 相似文献2.
3.
Marie GB Hansen Mette Christoffersen Line R Thuesen Morten R Petersen Anders M Bojesen 《Acta veterinaria Scandinavica》2010,52(1):3
Background
Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum are able to infect horses. However, the extend to which Danish horses are infected and seroconvert due to these two bacteria is unknown. The aim of the present study was to evaluate the seroprevalence of B. burgdorferi sensu lato and A. phagocytophilum in Danish horses.Methods
A total of 390 blood samples collected from all major regions of Denmark and with a geographical distribution corresponding to the density of the Danish horse population were analyzed. All samples were examined for the presence of antibodies against B. burgdorferi sensu lato and A. phagocytophilum by the use of the SNAP®4DX ® ELISA test.Results
Overall, 29.0% of the horses were seropositive for B. burgdorferi sensu lato whereas 22.3% were seropositive for A. phagocytophilum.Conclusions
Antibodies against B burgdorferi sensu lato and A. phagocytophilum are commonly found among Danish horses thus showing that Danish horses are frequently infected by these organisms.4.
O. I. Grabelnych O. A. Borovik E. L. Tauson T. P. Pobezhimova A. I. Katyshev N. S. Pavlovskaya N. A. Koroleva I. V. Lyubushkina V. Yu. Bashmakov V. N. Popov G. B. Borovskii V. K. Voinikov 《Biochemistry. Biokhimii?a》2014,79(6):506-519
Gene expression, protein synthesis, and activities of alternative oxidase (AOX), uncoupling proteins (UCP), adenine nucleotide translocator (ANT), and non-coupled NAD(P)H dehydrogenases (NDex, NDPex, and NDin) were studied in shoots of etiolated winter wheat (Triticum aestivum L.) seedlings after exposure to hardening low positive (2°C for 7 days) and freezing (?2°C for 2 days) temperatures. The cold hardening efficiently increased frost-resistance of the seedlings and decreased the generation of reactive oxygen species (ROS) during further cold shock. Functioning of mitochondrial energy-dissipating systems can represent a mechanism responsible for the decrease in ROS under these conditions. These systems are different in their response to the action of the hardening low positive and freezing temperatures. The functioning of the first system causes induction of AOX and UCP synthesis associated with an increase in electron transfer via AOX in the mitochondrial respiratory chain and also with an increase in the sensitivity of mitochondrial non-phosphorylating respiration to linoleic and palmitic acids. The increase in electron transfer via AOX upon exposure of seedlings to hardening freezing temperature is associated with retention of a high activity of NDex. It seems that NDex but not the NDPex and NDin can play an important role in maintaining the functional state of mitochondria in heterotrophic tissues of plants under the influence of freezing temperatures. The involvement of the mitochondrial energy-dissipating systems and their possible physiological role in the adaptation of winter crops to cold and frost are discussed. 相似文献
5.
A. S. Romanenko L. A. Lomovatskaya T. N. Shafikova G. B. Borovskii N. V. Krivolapova 《Journal of Phytopathology》2003,151(1):1-6
The interaction of extracellular polysaccharides (EPS) of the potato ring rot bacterial pathogen Clavibacter michiganensis ssp. sepedonicus (Spieck. et Kott.) Skaptason et Burkh. (Cms) with protoplasts isolated both from leaf cells of plants grown in vitro and microsomal membrane fractions obtained from cell suspension cultures of two potato (Solanum tuberosum L.) cultivars contrasted by their resistance to this pathogen was studied. The EPS intensively bind to protoplast surfaces and microsomal membranes of the susceptible cultivar but not to those of the resistant cultivar. Treatment with protease, excess of unlabelled EPS, and with dextran, did not lead to the binding of fluorochrome‐labelled EPS to protoplasts and microsomal membranes (from both cultivars). It is proposed that (a) a great number of receptors to EPS Cms are present in the plasma membranes of potato cells of susceptible cultivars, (b) these receptors contain proteinaceous sites exposed on the external side of the plasma membrane which participate in EPS binding, and (c) the plasma membranes of cells of resistant cultivars contain a small but sufficient quantity of receptors to EPS able to induce defensive responses in plants. 相似文献
6.
Pea seed mitochondria are endowed with a remarkable tolerance to extreme physiological temperatures
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Stupnikova I Benamar A Tolleter D Grelet J Borovskii G Dorne AJ Macherel D 《Plant physiology》2006,140(1):326-335
Most seeds are anhydrobiotes, relying on an array of protective and repair mechanisms, and seed mitochondria have previously been shown to harbor stress proteins probably involved in desiccation tolerance. Since temperature stress is a major issue for germinating seeds, the temperature response of pea (Pisum sativum) seed mitochondria was examined in comparison with that of mitochondria from etiolated epicotyl, a desiccation-sensitive tissue. The functional analysis illustrated the remarkable temperature tolerance of seed mitochondria in response to both cold and heat stress. The mitochondria maintained a well-coupled respiration between -3.5 degrees C and 40 degrees C, while epicotyl mitochondria were not efficient below 0 degrees C and collapsed above 30 degrees C. Both mitochondria exhibited a similar Arrhenius break temperature at 7 degrees C, although they differed in phospholipid composition. Seed mitochondria had a lower phosphatidylethanolamine-to-phosphatidylcholine ratio, fewer unsaturated fatty acids, and appeared less susceptible to lipid peroxidation. They also accumulated large amounts of heat shock protein HSP22 and late-embryogenesis abundant protein PsLEAm. The combination of membrane composition and stress protein accumulation required for desiccation tolerance is expected to lead to an unusually wide temperature tolerance, contributing to the fitness of germinating seeds in adverse conditions. The unique oxidation of external NADH at low temperatures found with several types of mitochondria may play a central role in maintaining energy homeostasis during cold shock, a situation often encountered by sessile and ectothermic higher plants. 相似文献
7.
Graskova I. A. Borovskii G. B. Kolesnichenko A. V. Voinikov V. K. 《Russian Journal of Plant Physiology》2004,51(5):621-626
Changes in the activity of peroxidase, a component of the NADPH oxidase signaling pathway, in potato cells were studied. This activity increased sharply during ring rot pathogenesis. Two mechanisms of peroxidase activation were distinguished. One of them was the enzyme de novo synthesis; it was characteristic of the potato cultivar susceptible to the pathogen. Another mechanism characteristic of the resistant cultivar included not only the enzyme synthesis but also the activation of preexisting enzyme molecules. Bacterial infection and exopolysaccharides secreted by the pathogen induced changes in the pattern of intra- and extracellular peroxidases of the susceptible cultivar. No changes were noted in the peroxidase patterns of the resistant cultivar. A sharp activation of the extracellular peroxidase of R
f 15 occurred in the infected or exopolysaccharide-treated cells of the resistant cultivar. 相似文献
8.
Rikhvanov E. G. Rachenko E. I. Varakina N. N. Rusaleva T. M. Borovskii G. B. Voinikov V. K. 《Russian Journal of Genetics》2004,40(4):341-347
Heat shock protein Hsp104 of Saccharomyces cerevisiae functions as a protector of cells against heat stress. When yeast are grown in media containing nonfermentable carbon sources, the constitutive level of this protein increases, which suggests an association between the expression of Hsp104 and yeast energy metabolism. In this work, it is shown that distortions in the function of mitochondria appearing as a result of mutation petite or after exposure of cells to the mitochondrial inhibitor sodium azide reduce the induction of Hsp104 synthesis during heat shock. Since the addition of sodium azide suppressed the formation of induced thermotolerance in the parent type and in mutant hsp104,the expression of gene HSP104 and other stress genes during heat shock is apparently regulated by mitochondria. 相似文献
9.
Polymorphism of Thermostable Proteins in Soft Wheat Seedlings during Low-Temperature Acclimation 总被引:5,自引:0,他引:5
Stupnikova I. V. Borovskii G. B. Antipina A. I. Voinikov V. K. 《Russian Journal of Plant Physiology》2001,48(6):804-810
The accumulation of thermostable stress proteins during hardening was studied in etiolated seedlings of spring (cvs. Rollo, Drott, Angara-86, and Tyumenskaya-80) and winter (moderately frost-resistant cv. Bezostaya-1 and highly frost-resistant cvs. Irkutskaya ozimaya and Zalarinka) wheat using one-dimensional SDS-PAGE. Hardening was performed at 4°C for nine days. Seedling tolerance to low subzero temperatures was estimated from electrolyte leakage and seedling survival after freezing. Hardening of all wheat genotypes tested resulted in the accumulation of thermostable cold-regulated (COR) polypeptides with mol wts of 209, 196, 169, 66, 50, and 41 kD. A densitometric analysis demonstrated a close correlation between the cultivar frost tolerance and the relative content of COR proteins, which evidently indicated the protective functions of the latter. These results led us to suggest that the level of specific protective agents, thermostable high-molecular-weight COR polypeptides in particular, determines the degree of plant frost resistance within a particular plant species. 相似文献
10.
A. S. Romanenko G. B. Borovskii I. V. Ukolova L. A. Lomovatskaya 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2010,4(2):162-170
Subcellular localization of dehydrins (dhn) in stem cell tissues of winter wheat seedlings (Triticulum aestivum L., cult. Irkutskaya ozimaya) was studied by immunoelectron microscopy. It was found that cold hardening at 4°C for 7 days
resulted in a duplication of the dhn quantity in the cells as compared with control conditions (22°C). The maximum increase
of the dhn content was observed in rough endoplasmic reticulum, mitochondria, cell walls, and intercellular spaces (3.8-,
3.0- and 2.8-fold, respectively); minimum increase was found in chloroplasts (1.4-fold). In the membrane compartments (mitochondria,
rough endoplasmic reticulum, chloroplasts) low-temperature stress caused an increase of dhn quantity not only near membranes
but also in the intermembrane space. A significant accumulation of dhn (2.5-fold) in the nucleus under low- temperature was
found. We conclude that cold hardening of the plant induces accumulation and translocation of dhn to the regions of inter-
and intracellular compartments that most require protection during the low-temperature stress. 相似文献