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Prashanth Kumar Koochana Abhinav Mohanty Suman Das Biswamaitree Subhadarshanee Suresh Satpati Anshuman Dixit Surendra Chandra Sabat Rabindra K. Behera 《Biochimica et Biophysica Acta (BBA)/General Subjects》2018,1862(5):1190-1198
Background
Ferritin detoxifies excess of free Fe(II) and concentrates it in the form of ferrihydrite (Fe2O3·xH2O) mineral. When in need, ferritin iron is released for cellular metabolic activities. However, the low solubility of Fe(III) at neutral pH, its encapsulation by stable protein nanocage and presence of dissolved O2 limits in vitro ferritin iron release.Methods
Physiological reducing agent, NADH (E1/2?=??330?mV) was inefficient in releasing the ferritin iron (E1/2?=?+183?mV), when used alone. Thus, current work investigates the role of low concentration (5–50?μM) of phenazine based electron transfer (ET) mediators such as FMN, PYO - a redox active virulence factor secreted by Pseudomonas aeruginosa and PMS towards iron mobilization from recombinant frog M ferritin.Results
The presence of dissolved O2, resulting in initial lag phase and low iron release in FMN, had little impact in case of PMS and PYO, reflecting their better ET relay ability that facilitates iron mobilization. The molecular modeling as well as fluorescence studies provided further structural insight towards interaction of redox mediators on ferritin surface for electron relay.Conclusions
Reductive mobilization of iron from ferritin is dependent on the relative rate of NADH oxidation, dissolved O2 consumption and mineral core reduction, which in turn depends on E1/2 of these mediators and their interaction with ferritin.General significance
The current mechanism of in vitro iron mobilization from ferritin by using redox mediators involves different ET steps, which may help to understand the iron release pathway in vivo and to check microbial growth. 相似文献
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