一个改进的下丘脑-垂体-甲状腺轴的数学模型

提出一个改进的下丘脑-垂体-甲状腺轴的数学模型.该模型既考虑了此分泌调节系统各激素之间的激活和反馈作用,也考虑了甲状腺激素与蛋白质结合的动力学过程.由该模型推导的结果与实验结果符合得很好.     

Re-Transurethral Resection Treatment for Non-invasive Bladder Tumor

The objective of this study was to compare surgical treatments for non-invasive bladder tumor. Hundred and forty patients with non-invasive bladder tumor were studied. Seventy-three patients were treated by transurethral resection of bladder tumor (TURBT) and Repeated-Transurethral Resection of Bladder Tumor (R-TURBT), while 67 by partial cystectomy. Operation time, blood loss, postoperative complications, and postoperative recurrence rate were better in the TURBT+R-TURBT group compared with the partial cystectomy group. Further, TURBT+R-TURBT offers advantages, such as simple surgical manipulation, less trauma, faster recovery, repeatedly performable procedure, and safety. In conclusion, this is an optimal therapy for treatment of non-invasive bladder tumor.     

下丘脑—垂体—性腺（男）轴内分泌调节控制的数理模型

本文在总结了大量实验事实的基础上提出了一个下丘脑—垂体—性腺(男性)轴内分泌的调节反馈模型。与已有的横型比较,本模型克服了它们的一些困难和不太合理之处,从而与实验符合得更好些。     

Chromosome-level genome of Tibetan naked carp (Gymnocypris przewalskii) provides insights into Tibetan highland adaptation

Gymnocypris przewalskii, a cyprinid fish endemic to the Qinghai-Tibetan Plateau, has evolved unique morphological, physiological and genetic characteristics to adapt to the highland environment. Herein, we assembled a high-quality G. przewalskii tetraploid genome with a size of 2.03 Gb and scaffold N50 of 44.93 Mb, which was anchored onto 46 chromosomes. The comparative analysis suggested that gene families related to highland adaptation were significantly expanded in G. przewalskii. According to the G. przewalskii genome, we evaluated the phylogenetic relationship of 13 schizothoracine fishes, and inferred that the demographic history of G. przewalskii was strongly associated with geographic and eco-environmental alterations. We noticed that G. przewalskii experienced whole-genome duplication, and genes preserved post duplication were functionally associated with adaptation to high salinity and alkalinity. In conclusion, a chromosome-scale G. przewalskii genome provides an important genomic resource for teleost fish, and will particularly promote our understanding of the molecular evolution and speciation of fish in the highland environment.     

MiR-101a-3p Attenuated Pilocarpine-Induced Epilepsy by Downregulating c-FOS

This study aimed to explore the effects and function of microRNA-101a-3p (miR-101a-3p) in epilepsy. Rat model of pilocarpine-induced epilepsy was established and the seizure frequency was recorded. Expression of miR-101a-3p and c-Fos in hippocampus tissues of Rat models were detected by qRT-PCR and western blot. Besides, we established a hippocampal neuronal culture model of acquired epilepsy using Mg²⁺ free medium to evaluate the effects of miR-101a-3p and c-Fos in vitro. Cells were transfected with miR-101a-3p mimic, si-c-FOS, miR-101a-3p?+?c-FOS and its corresponding controls. MTT assay was used to detect cell viability upon transfection. Flow cytometry was performed to determine the apoptosis rate. Western blot was performed to measure the protein expression of apoptosis-related proteins (Bcl-2, Bax, and cleaved caspase 3), autophagy-related proteins (LC3 and Beclin1) and c-FOS. The targeting relationship between miR-101a-3p and c-FOS was predicted and verified by TargetScan software and dual-luciferase reporter assay. The role of miR-101a-3p was validated using epilepsy rat models in vivo. Another Rat models of pilocarpine-induced epilepsy with miR-NC or miR-101a-3p injection were established to evaluate the effect of miR-101a-3p overexpression on epilepsy in vivo. MiR-101a-3p was downregulated while c-FOS was increased in hippocampus tissues of Rat model of pilocarpine-induced epilepsy. Overexpression of miR-101a-3p or c-FOS depletion promoted cell viability, inhibited cell apoptosis and autophagy. C-FOS was a target of miR-101a-3p and miR-101a-3p negatively regulated c-FOS expression to function in epilepsy. Overexpression of miR-101a-3p attenuated pilocarpine-induced epilepsy in Rats in vivo. This study indicated that miR-101a-3p could attenuate pilocarpine-induced epilepsy by repressing c-Fos expression.

     

森林转基因林木抽样策略的研究

转基因技术加快了林木遗传改良的进程。由于转基因林木存在潜在的生态风险,有必要对森林中转基因林木的分布等情况进行监测。基于无人机(unmanned aerial vehicles,UAV)获取的森林平面影像,提出了真实地形下森林转基因林木抽样监测的策略。首先根据检验参数来确定需要抽样的样本数,然后改进了两种算法分别进行不规则区域内常规的和有孔洞情况下的均匀抽样。与之前的抽样方法相比,本方法提供了更好的抽样准确性和均匀性,更适应森林区域的实际情况。     

Fishbone-Like High-Efficiency Low-Pass Plasmonic Filter Based on Double-Layered Conformal Surface Plasmons

In this work, we report a fishbone-like high-efficiency low-pass plasmonic filter based on a double-layered conformal surface plasmon waveguide (CSPW) which consists of double-layered symmetrical metal gratings (SMGs) of fishbone shape. Efficient mode conversion between the quasi-transverse electromagnetic (TEM) waves in the microstrip line and the conformal surface plasmons (CSPs) on the double-layered CSPW is realized by using gradient double-layered SMGs and impedance matching technique. Experimental results of the transmission and reflection coefficients of the straight sample show excellent loss-pass performance and agree well with the numerical simulations. The curved samples exhibit low radiation loss when the double-layered CSPW is conformal or even bent thanks to the high confinement of CSPs. The proposed structure can find potential applications in integrating conventional circuits with CSPs devices at microwave and terahertz frequencies.

     

胃电的非线性动力学分析

我们用非线性动力学的方法分析了两例健康人的胃电图（Ｅｌｅｃｔｒｏｇａｓｔｒｏｇｒａｍ,简称ＥＧＧ）,得出它的功率谱、计算了它的关联维数和最大Ｌｙａｐｕｎｏｖ指数。结果显示：它的功率谱为一有尖峰的连续谱;其关联维数介于６和７之间;它的最大Ｌｖａａｕｎｏｖ指数大于０。由此,我们可以初步推知ＥＧＧ不是随机噪声,它服从确定性的动力学规律。     

Mechanism of Monoclonal Antibody-Coupled Staphylococcus Superantigen-A Induced Apoptosis in Human Bladder Cancer Cells

To investigate the mechanism of apoptosis induced by BDI-1 monoclonal antibody (MAb) coupled Staphylococcal superantigen-A (SEA) in human bladder cancer cell line BIU-87. Human PBMC (effector cells) mediated cytotoxic killing of BIU-87 cells (target cells) was studied by culturing the BIU-87 cells in the presence of effector cells plus medium after their activation by treatment with SEA-targeted by MAb, SEA alone or vehicle (control). Proliferation and apoptosis of BIU-87 cells was measured after the treatments. Expression of Bax and Bcl-2 and cytokine concentration in co-culture supernatants were detected by Western blot and ELISA, respectively. Proliferation of MAb-targeted SEA BIU-87 cells decreased significantly (P < 0.05) as compared to control and SEA groups. Flow cytometry revealed apoptosis in SEA alone and more prominently in targeted-SEA treated in BIU-87 cells, which is significantly more than in controI cells (P < 0.05). In addition, Western blot analysis indicated that the ratio of Bax/Bcl-2 significantly increased by targeted SEA treatment, even at low concentration, as compared to cells treated with SEA alone or control cells (P < 0.05). However, there were no significant differences in IL-2, TNF-α and IFN-γ levels in the culture medium between SEA and targeted SEA groups, even though they are several folds higher than in control cells. SEA targeting by MAb significantly increases apoptosis in BIU-87 cells, possibly through the up-regulation of proapoptotic protein Bax and down regulation of antiapoptotic protein, Bcl-2.     

Deep-subwavelength Guiding and Superfocusing of Spoof Surface Plasmon Polaritons on Helically Grooved Metal Wire

Deep-subwavelength guiding and superfocusing of spoof surface plasmon polaritons (SSPPs) realized on a helically grooved metal wire at microwave frequencies are presented in this paper. Two smooth bridges with gradient helical grooves decorated on the cylindrical and conical metal wire are proposed and designed, respectively. High-efficiency and broadband mode conversion from the traditional guided waves to the SSPPs and superfocusing of SSPPs are reported. Numerical simulations quantitatively show that the amplitudes of electric field at the tip of the conical wire with gradient helical grooves can be magnified 50 times more than that of the input signal in broadband. Moreover, the second transition structure ensures that the depth of helical groove can be tuned flexibly and arbitrarily, making it compatible with all kinds of N-type coaxial connectors. Strong field concentration and superfocusing of these two structures can be easily extended to terahertz (THz) frequencies by tuning the geometrical parameters and can find important applications in sensing, spectroscopy and near-field imaging in the microwave and THz frequencies.