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Marco Guida Paolo Losanno Cannavacciuolo Mara Cesarano Marco Borra Elio Biffali Raffaella D’Alessandro Bruna De Felice 《Journal of applied genetics》2014,55(3):403-415
Landslides are a significant component of natural disasters in most countries around the world. Understanding these destructive phenomena through the analysis of possible correlations between microbial communities and the alteration of the soil responsible for landslides is important in order to reduce their negative consequences. To address this issue, bacterial and fungal communities in soils triggering landslides in Termini-Nerano and Massa Lubrense-Nerano (Naples, Italy) were analysed by genetic profiling techniques. Fingerprints were generated by single-strand conformation polymorphisms (SSCP) and random amplified polymorphic DNA (RAPD). The microbial community in both soil types was enriched in species which could contribute to the degradation process occurring during landslides, forming biofilms and leading to the transformation or the formation of minerals. Indeed, some of the identified bacteria were found to favour the transformation of clay minerals. These findings suggest a possible relationship between bacterial and fungal community-colonising soils and the occurrence of landslides. 相似文献
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De Luca Pasquale Iaconis Daniela Biffali Elio Enza Coluccia de Magistris Laura Riegler Gabriele Pappalardo Diego Amato Maria Rosaria Iardino Patrizia Montanino Concetta De Felice Bruna 《Molecular biology reports》2021,48(11):7087-7093
Molecular Biology Reports - In adulthood the activity of the lactase enzyme is inherited as autosomal dominant form associated to Single nucleotide polymorphisms (SNPs). The present research was... 相似文献
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TTF-2, a new forkhead protein, shows a temporal expression in the developing thyroid which is consistent with a role in controlling the onset of differentiation. 总被引:8,自引:0,他引:8
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M Zannini V Avantaggiato E Biffali M I Arnone K Sato M Pischetola B A Taylor S J Phillips A Simeone R Di Lauro 《The EMBO journal》1997,16(11):3185-3197
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Maria Sirakov Ilaria Zarrella Marco Borra Francesca Rizzo Elio Biffali Maria Ina Arnone Graziano Fiorito 《BMC molecular biology》2009,10(1):70
Background
Quantitative real-time polymerase chain reaction (RT-qPCR) is valuable for studying the molecular events underlying physiological and behavioral phenomena. Normalization of real-time PCR data is critical for a reliable mRNA quantification. Here we identify reference genes to be utilized in RT-qPCR experiments to normalize and monitor the expression of target genes in the brain of the cephalopod mollusc Octopus vulgaris, an invertebrate. Such an approach is novel for this taxon and of advantage in future experiments given the complexity of the behavioral repertoire of this species when compared with its relatively simple neural organization. 相似文献7.
Tristano Bacchetti De Gregoris Marco Borra Elio Biffali Thomas Bekel J Grant Burgess Richard R Kirby Anthony S Clare 《BMC molecular biology》2009,10(1):62
Background
Balanus amphitrite is a barnacle commonly used in biofouling research. Although many aspects of its biology have been elucidated, the lack of genetic information is impeding a molecular understanding of its life cycle. As part of a wider multidisciplinary approach to reveal the biogenic cues influencing barnacle settlement and metamorphosis, we have sequenced and annotated the first cDNA library for B. amphitrite. We also present a systematic validation of potential reference genes for normalization of quantitative real-time PCR (qRT-PCR) data obtained from different developmental stages of this animal. 相似文献8.
Emilia Vuttariello Marco Borra Celeste Calise Elvira Mauriello Stefano Greggi Aldo Vecchione Elio Biffali Gennaro Chiappetta 《Molecular biotechnology》2013,54(3):954-960
Hereditary cancers account for approximately 10 % of breast and ovarian cancers. Mutations of the BRCA1 and BRCA2 genes, encoding two proteins involved in DNA repair, underlie most cases of such hereditary cancers. Women with BRCA mutations develop breast cancer in 50–80 % of cases and ovarian cancer in 10–40 % of cases. Assessing BRCA mutational status is needed to direct the clinical management of women with predisposition to these hereditary cancers. However, BRCA screening constitutes a bottleneck in terms of costs and time to deliver results. We developed a PCR-based assay using 73 primer pairs covering the entire coding regions of BRCA1 and BRCA2. PCR primers, containing at the 5’ end the universal M13 primer sequences, were pre-spotted in 96-well plates. Following PCR, direct sequencing was performed using M13 primers, allowing to standardize the conditions. PCR amplification and sequencing were successful for each amplicon. We tested and validated the assay on 10 known gDNAs from patients with Hereditary breast and ovarian cancer (HBOC). Our strategy is a promising time and cost-effective method to detect BRCA mutations in the clinical setting, which is essential to formulate a personalized therapy for patients with HBOC. 相似文献
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