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1.
Marie GB Hansen Mette Christoffersen Line R Thuesen Morten R Petersen Anders M Bojesen 《Acta veterinaria Scandinavica》2010,52(1):3
Background
Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum are able to infect horses. However, the extend to which Danish horses are infected and seroconvert due to these two bacteria is unknown. The aim of the present study was to evaluate the seroprevalence of B. burgdorferi sensu lato and A. phagocytophilum in Danish horses.Methods
A total of 390 blood samples collected from all major regions of Denmark and with a geographical distribution corresponding to the density of the Danish horse population were analyzed. All samples were examined for the presence of antibodies against B. burgdorferi sensu lato and A. phagocytophilum by the use of the SNAP®4DX ® ELISA test.Results
Overall, 29.0% of the horses were seropositive for B. burgdorferi sensu lato whereas 22.3% were seropositive for A. phagocytophilum.Conclusions
Antibodies against B burgdorferi sensu lato and A. phagocytophilum are commonly found among Danish horses thus showing that Danish horses are frequently infected by these organisms.2.
Ribas GS Biancini GB Mescka C Wayhs CY Sitta A Wajner M Vargas CR 《Cellular and molecular neurobiology》2012,32(1):77-82
Propionic (PA) and methylmalonic (MMA) acidurias are inherited disorders caused by deficiency of propionyl-CoA carboxylase
and methylmalonyl-CoA mutase, respectively. Affected patients present acute metabolic crises in the neonatal period and long-term
neurological deficits. Treatments of these diseases include a protein restricted diet and l-carnitine supplementation. l-Carnitine is widely used in the therapy of these diseases to prevent secondary l-carnitine deficiency and promote detoxification, and several recent in vitro and in vivo studies have reported antioxidant
and antiperoxidative effects of this compound. In this study, we evaluated the oxidative stress parameters, isoprostane and
di-tyrosine levels, and the antioxidant capacity, in urine from patients with PA and MMA at the diagnosis, and during treatment
with l-carnitine and protein-restricted diet. We verified a significant increase of isoprostanes and di-tyrosine, as well as a significant
reduction of the antioxidant capacity in urine from these patients at diagnosis, as compared to controls. Furthermore, treated
patients presented a marked reduction of isoprostanes and di-tyrosine levels in relation to untreated patients. In addition,
patients with higher levels of protein and lipid oxidative damage, determined by di-tyrosine and isoprostanes levels, also
presented lower urinary concentrations of total and free l-carnitine. In conclusion, the present results indicate that treatment with low protein diet and l-carnitine significantly reduces urinary biomarkers of protein and lipid oxidative damage in patients with disorders of propionate
metabolism and that l-carnitine supplementation may be specially involved in this protection. 相似文献
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Camila Simioni Vanzin Vanusa Manfredini Ana Eveline Marinho Giovana Brondani Biancini Graziela Schmitt Ribas Marion Deon Angela Terezinha de Souza Wyse Moacir Wajner Carmen Regla Vargas 《Gene》2014
High blood levels of homocysteine (Hcy) are found in patients affected by homocystinuria, a genetic disorder caused by deficiency of cystathionine β-synthase (CBS) activity, as well as in nutritional deficiencies (vitamin B12 or folate) and in abnormal renal function. We previously demonstrated that lipid and protein oxidative damage is increased and the antioxidant defenses diminished in plasma of CBS-deficient patients, indicating that oxidative stress is involved in the pathophysiology of this disease. In the present work, we extended these investigations by evaluating DNA damage through the comet assay in peripheral leukocytes from CBS-deficient patients, as well as by analyzing of the in vitro effect of Hcy on DNA damage in white blood cells. We verified that DNA damage was significantly higher in the CBS-deficient patients under treatment based on a protein-restricted diet and pyridoxine, folic acid, betaine and vitamin B12 supplementation, when compared to controls. Furthermore, the in vitro study showed a concentration-dependent effect of Hcy inducing DNA damage. Taken together, the present data indicate that DNA damage occurs in treated CBS-deficient patients, possibly due to high Hcy levels. 相似文献
5.
Martin Kolisko Ivan Cepicka Vladimir Hampl Jessica Leigh Andrew J Roger Jaroslav Kulda Alastair GB Simpson Jaroslav Flegr 《BMC evolutionary biology》2008,8(1):205
Background
Fornicata is a relatively recently established group of protists that includes the diplokaryotic diplomonads (which have two similar nuclei per cell), and the monokaryotic enteromonads, retortamonads and Carpediemonas, with the more typical one nucleus per cell. The monophyly of the group was confirmed by molecular phylogenetic studies, but neither the internal phylogeny nor its position on the eukaryotic tree has been clearly resolved. 相似文献6.
Alessandro Finazzi-Agrò Adriana Menichelli Mauro Persiani Giuseppino Biancini Domenico Del Principe 《Biochimica et Biophysica Acta (BBA)/General Subjects》1982,718(1):21-25
The release of hydrogen peroxide from human blood platelets after stimulation with particulate membrane-perturbing agents has been determined by fluorescence using scopoletin as the detecting agent. Platelet suspensions containing less than 1 polymorphonuclear leukocyte/108 platelets showed a significant release of hydrogen peroxide (6.11 nmol/109 platelets per 20 min, S.D., 0.26, n=9) after addition of zymosan or latex particles, compared to unstimulated platelets. The release of hydrogen peroxide was only observed when the scopoletin was added to the platelet suspensions during the stimulation. Any attempt to determine hydrogen peroxide release in the supernatant at the end of the incubation with zymosan or latex failed. A NADH-dependent production of hydrogen peroxide was observed by measuring the difference of oxygen uptake in the presence and absence of catalase (500 units), which was not inhibited by potassium cyanide (1 mM). By this method the NADH-dependent cyanide-insensitive peroxide production and release was 6.0 nmol/109 platelets per 20 min from resting platelets (S.D., 2, n=6) vs. 15 nmol/109 platelets per 20 min from stimulated platelets (S.D., 2, n=6). 相似文献
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Giovana B. Biancini Camila S. Vanzin Daiane B. Rodrigues Alethéa G. Barschak Roberto Giugliani Carmen R. Vargas 《生物化学与生物物理学报:疾病的分子基础》2012,1822(2):226-232
Fabry disease is an X-linked inborn error of glycosphingolipid catabolism due to deficient activity of α-galactosidase A that leads to accumulation of the enzyme substrates, mainly globotriaosylceramide (Gb3), in body fluids and lysosomes of many cell types. Some pathophysiology hypotheses are intimately linked to reactive species production and inflammation, but until this moment there is no in vivo study about it. Hence, the aim of this study was to investigate oxidative stress parameters, pro-inflammatory cytokines and Gb3 levels in Fabry patients under treatment with enzyme replacement therapy (ERT) and finally to establish a possible relation between them. We analyzed urine and blood samples of patients under ERT (n = 14) and healthy age-matched controls (n = 14). Patients presented decreased levels of antioxidant defenses, assessed by reduced glutathione (GSH), glutathione peroxidase (GPx) activity and increased superoxide dismutase/catalase (SOD/CAT) ratio in erythrocytes. Concerning to the damage to biomolecules (lipids and proteins), we found that plasma levels of malondialdehyde (MDA) and protein carbonyl groups and di-tyrosine (di-Tyr) in urine were increased in patients. The pro-inflammatory cytokines IL-6 and TNF-α were also increased in patients. Urinary Gb3 levels were positively correlated with the plasma levels of IL-6, carbonyl groups and MDA. IL-6 levels were directly correlated with di-Tyr and inversely correlated with GPx activity. This data suggest that pro-inflammatory and pro-oxidant states occur, are correlated and seem to be induced by Gb3 in Fabry patients. 相似文献
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Estimates of DNA and protein sequence divergence: an examination of some assumptions 总被引:2,自引:3,他引:2
Some of the assumptions underlying estimates of DNA and protein sequence
divergence are examined. A solution for the variance of these estimates
that allows for different mutation rates and different population sizes in
each species and for an arbitrary structure in the initial population is
obtained. It is shown that these conditions do not strongly affect
estimates of divergence. In general, they cause the variance of divergence
to be smaller than a binomial variance. Thus, the binomial variance that is
usually assumed for these estimates is safely conservative. It is shown
that variability in the mutation rate among sites can have an effect as
large as or larger than variability in the mutation rate among bases.
Variability in the mutation rate among bases and among sites causes the
number of substitutions between two sequences to be underestimated. Protein
and DNA sequences from several species are collected to estimate the
variability in mutation rates among sites. When many homologous sequences
are known, standard methods to estimate this variability can be used. The
estimates of this variability show that this factor is important when
considering the spectrum of spontaneous mutations and is strongly reflected
in the divergence of sequences. Smaller variability is found for the third
position of codons than for the first and second codon positions. This may
be because of less selective constraints on this position or because the
third position has been saturated with mutations for the sequences
examined.
相似文献
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