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Bialek-Bylka Grazyna E. Fujii Ritsuko Chen Chun-Hai Oh-oka Hirozo Kamiesu Akihisa Satoh Kazuhiko Koike Hiroyuki Koyama Yasushi 《Photosynthesis research》1998,58(2):135-142
Carotenoids were extracted, at 4 °C in complete darkness and under nitrogen atmosphere, from the reaction center (RC) of a green-sulfur bacterium and the Photosystem (PS) I RC of a cyanobacterium; each extract was subjected to high-performance liquid chromatography (HPLC) using an apparatus equipped with a two-dimensional diode-array detector in order to spectroscopically identify cis–trans carotenoids while performing HPLC analysis. In the extract from the RC of Chlorobium tepidum, 15-cis and all-trans--carotenes as well as 13-cis-, 15-cis- and all- trans-chlorobactenes (in the order of elution) were identified, whereas in the extract from the PS I RC of Synechococcus vulcanus, 15-cis-, all-trans- and 9-cis--carotenes were found. Thus, the universal presence of 15- cis carotenoids in the 'iron sulfur-type' RCs has been shown in addition to the previous cases of the 'quinone-type' RCs. 相似文献
2.
Central-Cis isomers of lutein found in the major light-harvesting complex of Photosystem II (LHC IIb) of higher plants 总被引:1,自引:0,他引:1
Bialek-Bylka Grazyna E. Sakano Yoko Mizoguchi Tadashi Shimamura Toshio Phillip Denise Koyama Yasushi Young Andrew J. 《Photosynthesis research》1998,56(3):255-264
Lutein (,-carotene-3,3-diol) is the major carotenoid of the light-harvesting systems of higher plants. Lutein was isolated at 4°C and in complete darkness from the bulk light-harvesting complex of Photosystem II of spinach (LHC IIb) and from BBY particles. Separation using normal-phase HPLC (with 2D detection) in comparison to the authentic isomers (prepared by iodine-sensitised isomerization) showed the presence of a number of geometrical isomers of this xanthophyll in PS II, namely all-trans (the major component); 13-cis, 13-cis and 15-cis-lutein. Iodine-sensitised photo-isomerization of all-trans lutein produced six geometrical isomers of lutein as determined by HPLC. The configuration of five of these isomers was determined by 1H-NMR to be all-trans, 9-cis, 9-cis, 13-cis and 13-cis. In addition, small amounts of another isomer have been tentatively identified to be 15-cis lutein on the basis of its electronic absorption spectrum. The possible functional significance of the presence of cis-isomers of this carotenoid in LHC IIb is discussed. 相似文献
3.
The clear vibrational structure of fluorescence spectrum of β-carotene in the solvent is reported for the first time at room
temperature. This finding is in good agreement with recently discovered covalent 3 1A
g
−
new carotenoid state. The fluorescence yield of β-carotene in ionic liquid (1-methyl-3-octyloxymethylimidazolium tetrafluoroborate)
is around hundred times higher than in standard solvent n-hexane. The all-trans and 15-cis β-carotene fluorescence yields in ionic liquid are 1.96±0.03 and 2.53±0.03 %, respectively. The ionic liquid is a very useful
tool for modelling photosynthetic system in situ. We present the electronic absorption data of β-carotene in ionic liquids (so called neoteric solvents) with special interest
in the absorption changes as a function of temperature in the range 0–90 °C (273–363 K). Ionic liquids are also very good
medium for temperature study, because they are not changing up to several hundred °C and also not evaporating during heating.
The relationship between spectral characteristics of β-carotene in new generation solvents with increasing and decreasing
temperature is evaluated. The energy value of the ionic state 1 1B
u
+
of synthetic β-carotene in ionic liquids exhibits a linear and temperature reversible dependence on temperature up to 30
°C (303 K) and up to 40 °C (313 K) for 15-cis and all-trans β-carotenes, respectively. This is valid for both 0-0 and 0-1 transitions. 相似文献
4.
Grazyna E. Bialek-Bylka Tetsuo Hiyama Kenji Yumoto Yasushi Koyama 《Photosynthesis research》1996,49(3):245-250
-Carotene was extracted from spinach Photosystem I reaction centers (one consisting of the Psa A, B, C, D and E subunits and the other consisting of the Psa A and B subunits alone), and the extract was subjected to high-pressure liquid chromatography using an apparatus equipped with a two-dimensional diode-array detector; all the procedures were performed at 4 °C in complete darkness. Both 15-cis and all-trans--carotene were identified in the extract by means of electronic absorption spectroscopy. Thus, universal presence of 15-cis carotenoid in the reaction centers of purple photosynthetic bacteria and of spinach Photosystem I and Photosystem II has been shown.Abbreviations Chl-
chlorophyll
- DEAE-
diethylaminoethyl
- DMF-
dimethylformamide
- HPLC-
high pressure liquid chromatography
- LHC-
light-harvesting complex
- PS-
Photosystem
- RC-
reaction center
- RCa,b-
reaction center consisting of Psa A and B subunits alone
- RCa-e-
reaction center consisting of Psa A, B, C, D and E subunits 相似文献
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