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1.
F F Dias M K Shaikh Y B Bhatt D C Modi V R Subramanyam 《The Journal of applied bacteriology》1986,60(4):271-275
Mutants of Bacillus amyloliquefaciens resistant to at least 10 micrograms/ml of tunicamycin were isolated and shown to be pleiotropic. The mutants were more resistant to streptomycin, chloramphenicol, kanamycin and neomycin than was the parent strain but less resistant to penicillin G and tetracycline. They were more autolytic, presumably due to an altered cell wall. The mutants produced reduced levels of amylase, penicillinase and both metal and serine protease besides having an enhanced sporulation frequency and being more motile. 相似文献
2.
Ankita Solanki Shreya R. Savla Maheshkumar R. Borkar Lokesh K. Bhatt 《Journal of biochemical and molecular toxicology》2023,37(5):e23322
Mammalian target of Rapamycin C1 (mTORC1) inhibition limits plaque progression in atherosclerosis. The present study evaluated the protective effect of sulfamethizole on poloxamer 407-induced atherosclerotic neointima formation in C57BL/6 mice via mTOR inhibition. Poloxamer 407 (P-407) (0.5 g/kg body weight) was administered intraperitoneally to male C57BL/6 mice every third day for 148 days to induce chronic hyperlipidemia. From Day 121 to 148, animals were additionally administered Sulfamethizole (5, 10, and 50 mg/kg, p.o.), Rapamycin (0.5 mg/kg, positive control), or vehicle (1 ml/kg). Plasma lipid levels were measured on Days 120 and 148. Upon sacrifice, histological studies were performed, and aortic tissue interleukin (IL)-6, tumor necrosis factor-α (TNF-α), and mTOR levels were evaluated. A molecular docking study was carried out to mimic the interaction of sulfamethizole with mTOR protein. Chronic P-407 administration significantly (p < 0.001) elevated plasma lipid levels, compared with those of the normal control group. Chronic hyperlipidemia resulted in increased tunica intima thickness, collagen deposition, and IL-6, TNF-α, and mTOR levels. Treatment with Sulfamethizole attenuated these parameters significantly in a dose-dependent manner. Molecular docking studies showed a significant interaction of Sulfamethizole with mTOR. In conclusion, this study suggests that sulfamethizole significantly limits poloxamer 407-induced atherosclerotic neointima formation in C57BL/6 mice via mTOR inhibition. 相似文献
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4.
Arvind Kumar Bhatt Tek Chand Bhalla Hari Om Agrawal N. Sharma 《Letters in applied microbiology》1992,15(1):1-4
An extracellular chitosanase produced by Rhodotorula gracilis CFR-1 that catalyses a limited degradation of chitosan with no detectable generation of glucosamine or reducing groups was identified. Ultracentrifugation, polyacrylamide gel electrophoresis and gel permeation studies suggest that chitosan of average molecular mass 36000 Da was reduced by the enzymic catalysis to nearly one-fourth this size without further hydrolysis of the products. The enzyme, produced constitutively by this yeast, was partially purified and some of its properties were studied. 相似文献
5.
D C Modi Y B Bhatt M K Shaikh V R Subramanyam F F Dias 《The Journal of applied bacteriology》1986,61(4):315-318
Anilogel-E, a cross-linked starch, can be used with distinct advantages where native starch or soluble starch are conventionally used, e.g. in scoring for amylolytic organisms, as an ingredient of fermentation media, and in enhancing protoplast regeneration. It is particularly useful for the direct visualization of amylase producing micro-organisms on solid media, making prior replication of colonies unnecessary. 相似文献
6.
Short-term metabolic and concomitant morphologic effects of streptozotocin diabetes on isoproterenol-induced myocardial infarction
was studied in Wistar rats, Of particular significance was the observation that myocardial infarction in concert with diabetes
brought about a distinctive exacerbation of the severity and complexity of the histopathological lesions. Of all the biochemical
parameters, serum glucose and free fatty acids registered maximum elevation and serum lactate and cardiac glycogen levels
a maximum reduction. Among the lipoproteins, an inverse relationship was found between high density lipoproteins and low density
and very low density lipoproteins; while high density lipoproteins, ratio of high density lipoprotein to low density lipoprotein
and the percentage of high density lipoprotein were decreased, there was a significant increase in low density lipoprotein
concentration and percentage values of low density and very low density lipoproteins. In diabetes, the B cell of the endocrine
pancreas depicted selective necrosis. Loss of insulin granules and wide-spread necrobiosis of cellular elements of the pancreatic
islets were observed, respectively, in myocardial infarction and in diabetes plus myocardial infarction combinations. Pathological
evidence of chemical-induced mild toxicity was present in the exocrine parenchyma. Mitotic features and the presence of centroacinar
cells in the damaged Langerhans’ islets supposedly formed the basis of regeneration of the tissue in diabetes, with or without
vascular complications 相似文献
7.
The variations observed during earlier studies in the activity of NADP+-isocitrate dehydrogenase (EC. 1.1.1.42) in a strain of Aspergillus niger were found to be related to the extent of washing of mycelium. As a result the mycelium washed four times with phosphate buffer (0.05 M, pH 7.5), the enzyme activity present in 4 and 8 days old fungal mycelia increased five- and two-fold, respectively. In vivo studies showed a complete loss of enzyme activity in mycelia resuspended in HCl-KCl buffer (0.02 M, pH 2.2) containing citric acid (13 mM or more). The in vitro studies revealed 50% loss of enzyme activity in presence of 3.6 to 5.2 mM citric acid. However, in case of Aspergillus niger ATCC 1015, which produced less citric acid than the above strain, a much higher citric acid concentration (13 to 26 mM) was required to cause 50% loss of enzyme activity. These findings suggest a correlation between citric acid inhibition of NADP+-isocitrate dehydrogenase and the ability of A. niger to accumulate citric acid in the medium. 相似文献
8.
Summary The studies on uptake of nitrogen, phosphorus and potassium by two morphologically contrasting varieties of cotton MCU-1 and
PRS-72 (bothGossypium hirsutum L.) at weekly intervals, showed that till the bursting of early formed bolls, the former type accumulated these nutrients
in larger quantities in leaves and stem than in the fruiting parts unlike the latter type where differences in nutrient contents
of these organs were very narrow till the active bolling phase. From flowering onwards, the fruiting parts of PRS-72 contained
more phosphorus. Because of its bushy habit more dry matter accumulated and more nutrients remained unutilised in leaves and
stem of MCU-1 at harvest compared with the short-branch variety PRS-72. Though both the varieties gave the same yield of seed
cotton per unit area, PRS-72 removed considerably less quantities of nitrogen, phosphorus and pottassium. The short fruiting
branch character in cotton therefore appears to contribute substantially towards balanced and economic utilization of nutrients. 相似文献
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