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Trabelsi El Bahri Bouzid Sadok Bouzid Monji Elloumi Nedra Belfeleh Zina Benabdallah Abdallah Ghezel Rachida 《Journal of Plant Biology》2003,46(3):173-180
We induced somatic embryogenesis from the cotyledon segments ofOlea europaea (L) cvs. ‘Chetoui’, ‘Chemleli’, and ‘Arbequina’. Calli were established from all three cultvars on OMc media supplemented
with IBA and 2i-R The greatest success was obtained with media that contained zero or low concentrations of growth regulators.
High levels of hormones (i.e.,>0.5 mgL-1 IBA and 2i-P) inhibited embryogenesis. Embryos at different maturation stages were observed with continuously proliferating
secondary embryogenesis. Abnormally shaped embryos and teratoma were also noted. Four weeks was the optimal incubation period
for inducing embryogenesis on the auxin-containing medium. In addition, 30 to 40 gL-1 sucrose was more effective than glucose in stimulating the growth and maturation of somatic embryos. Embryogeic efficiency
was also higher when multivariate combinations of nitrogen sources (inorganic and organic nitrogen forms) were used. The plantlets
that were derived from our germinating somatic embryos were similar to those obtained from axillary buds. 相似文献
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El Bahri Trabelsi Selima Naija Nedra Elloumi Zina Belfeleh Monji Msellem Rachida Ghezel Sadok Bouzid 《Acta Physiologiae Plantarum》2011,33(2):319-324
Somatic embryogenesis of olive Olea europaea (L.) ‘Chetoui’ was studied using cell suspension cultures initiated from mature leaf-derived calli. Calli were developed
on half-strength MS medium supplemented with 10 μM NAA and 2.25 μM 2i-P in the dark. Different combinations of three plant
growth regulators (2,4-D, NAA and zeatin) were tested to determine cell proliferation and somatic embryogenesis induction
and differentiation. Embryogenic suspension cultures were established in olive-modified medium for embryogenesis (OMe) containing
2.5 μM 2,4-D and 2.5 μM zeatin. Pre-globular and globular embryos were induced from mature olive tissue in liquid medium.
In addition, the nitrogen form as inorganic (reduced; (NH4)2SO4 or oxidized; KNO3) and organic (CH) was used separately or in combination to improve the cell growth and proliferation. The most effective
growth rate and cell proliferation were obtained with the medium containing inorganic and organic nitrogen forms. 相似文献
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