Delivery of CRISPR/Cas Components into Higher Plant Cells for Genome Editing

Russian Journal of Plant Physiology - CRISPR/Cas genome editing of plants is realized in three basic variants, including knockout mutations as indels, insertion of alien DNA fragments, and base...     

Usage of strain-producers of adhesin RapA1 from <Emphasis Type="Italic">Rhizobium leguminosarum</Emphasis> for the creation of binary biofertilizers

Binary biofertilizers consisting of a rhizobial strain specific to the plant Rhizobium leguminosarum and a strain-producer of the RapA1 adhesin protein, which enhances the adhesion of rhizobacteria to the plant roots, have been designed. A system consisting of highly productive bacteria strains and additional strains that enhance the action of the main component have been obtained.     

Modern Approaches to Differentiation of Live and Dead Bacteria Using Selective Amplification of Nucleic Acids

Microbiology - Specific amplification of nucleic acids is a convenient and quick alternative to the culture-based method of detecting bacterial cells. However, conventional PCR and other...     

Carbohydrate-binding sequences of the lectin genes in leguminous plants from the Galegeae and Hedysareae Tribes

The carbohydrate-binding sequences (CBS) of the lectin genes from legume plants of the genera Astragalus Lam., Oxytropis DC., and Hedysarum L. were determined. Computer-assisted analysis of nucleotide and predicted amino acid sequences of the lectin gene fragments examined revealed a high homology level between their CBS. At the same time, the CBS of Astragalus and Oxytropis were considerably different from the lectin gene CBS in the earlier examined representatives of the tribe Galegeae, Caragana frutex and C. arborescens. This fact probably points to the differences in the carbohydrate-binding specificity of the proteins examined, which can eventually affect their functional activity.     

Random Priming PCR Strategies for Identification of Multilocus DNA Polymorphism in Eukaryotes

A historical review of the advent and improvement of the methods for detecting multilocus DNA polymorphism that do not require preliminary knowledge of the individual gene and complete genome sequences of eukaryotes is presented. The first group of these methods includes approaches based on the use of primers with arbitrary sequence (random priming). Another group of methods to detect DNA polymorphism is based on the use of primers that consist of short repetitive sequences having anchor nucleotides at the 5'- or 3'-ends that position the annealing sites of these primers (microsatellite priming). Another approach for revealing polymorphism that does not require knowledge of the DNA sequence is based on cleavage of total DNA by a combination of restriction endonucleases (random cleavage) accompanied by PCR amplification. Considerable attention is paid to the opportunities of using these approaches to detect DNA polymorphism in the form of converting the obtained data to digital format and creation of integrative databases for all organisms, regardless of the methods used.     

Effect of rosR Gene Overexpression on Biofilm Formation by Rhizobium leguminosarum

Microbiology - The regulatory protein encoded by the rosR gene is involved in the processes of adaptation of root nodule bacteria Rhizobium leguminosarum to changes in environmental conditions. It...     

Inverse PCR-based site-directed mutagenesis of nucleotide sequences coding for carbohydrate-binding fragments of legume lectins

A new method of site-directed mutagenesis was developed to allow manipulation with extended plasmid-cloned gene fragments irrespective of their position and the presence of restriction sites. The method was used to obtain chimeric constructs encoding a Pisum sativum lectin with the native carbohydrate-binding region replaced by its counterpart from other legumes. The method can be used in plasmid construction to clone a coding gene fragment under the control of a promoter in a certain reading frame.     

The Composition of Lotus corniculatus Root Nodule Bacteria Depending on the Host Plant Vegetation Stage

Microbiology - The legume-Rhizobium symbiosis is a unique natural phenomenon responsible for providing a legume plant with mineral nitrogen fixed from the atmosphere. In the temperate climatic...