全文获取类型
收费全文 | 455篇 |
免费 | 67篇 |
出版年
2021年 | 9篇 |
2019年 | 9篇 |
2018年 | 10篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2015年 | 10篇 |
2014年 | 17篇 |
2013年 | 11篇 |
2012年 | 22篇 |
2011年 | 21篇 |
2010年 | 19篇 |
2009年 | 13篇 |
2008年 | 18篇 |
2007年 | 18篇 |
2006年 | 20篇 |
2005年 | 23篇 |
2004年 | 13篇 |
2003年 | 20篇 |
2002年 | 17篇 |
2001年 | 18篇 |
2000年 | 19篇 |
1999年 | 13篇 |
1998年 | 4篇 |
1997年 | 3篇 |
1996年 | 7篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 6篇 |
1992年 | 8篇 |
1991年 | 11篇 |
1990年 | 7篇 |
1989年 | 12篇 |
1988年 | 7篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 5篇 |
1982年 | 8篇 |
1981年 | 5篇 |
1980年 | 7篇 |
1979年 | 9篇 |
1978年 | 7篇 |
1977年 | 9篇 |
1976年 | 9篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1972年 | 6篇 |
1971年 | 6篇 |
1970年 | 3篇 |
排序方式: 共有522条查询结果,搜索用时 15 毫秒
1.
2.
R J Baskin 《The Journal of general physiology》1965,49(1):9-15
Total oxygen consumption following contraction at controlled shortening velocities was determined for ten twitches of frog sartorius muscle. Under the conditions of this investigation maximum oxygen consumption occurred at approximately 30 mm/sec. shortening velocity and decreased for larger and smaller velocities. Mechanical efficiency was calculated and found to show a maximum at approximately 20 mm/sec. shortening velocity. The relationship between this study and a similar investigation in which heat changes are measured as a function of shortening velocity is discussed. 相似文献
3.
Entirely etiolated pea seedlings (Pisum sativum, L. cv Alaska) were tested for a phototropic response to short pulses of unilateral blue light. They responded with small curvatures resembling in fluence-dependence and kinetics of development a phytochrome-mediated phototropic response previously described in maize mesocotyls. Irradiations from above with saturating red or far-red light, either immediately before or after the unilateral phototropic stimulus, strongly reduced or eliminated subsequent positive phototropic curvature. Only blue light from above, however, entirely eliminated curvature at all fluences of stimulus. It is concluded that the phototropism is primarily a result of phytochrome action. 相似文献
4.
Barbara J. Wilcox Eric S. Corp Daniel M. Dorsa Dianne P. Figlewicz M. R. C. Greenwood Stephen C. Woods Denis G. Baskin 《Peptides》1989,10(6):1159-1164
Recent reports have suggested that the obesity and hyperphagia of the genetically obese Zucker rat may be related to defective insulin action or binding in the hypothalamus. We used quantitative autoradiography to determine if insulin binding is altered in specific hypothalamic nuclei associated with food intake. Insulin binding was measured in the arcuate (ARC), dorsomedial (DMN), and ventromedial (VMN) hypothalamic nuclei of 3–4-month-old lean (Fa/Fa) and genetically obese (fa/fa) Zucker rats. A consistently reproducible 15% increase in the total specific binding of 0.1 nM [125I]-insulin was found in the ARC of the obese genotype. A slight increase in insulin binding in the DMN was also found. No difference in specific insulin binding was found between genotypes in the VMN. Nonlinear least squares analysis of competitive binding studies showed that the Kd of the ARC insulin binding site was 33% higher in the lean rats than in the obese rats, indicating an increased affinity for insulin. No difference in site number (Bmax) was found in the ARC, DMN or VMN, and no evidence was found for reduced insulin binding in the hypothalamus of the obese (fa/fa) genotype. The results suggest that hyperphagia and obesity of the obese (fa/fa) Zucker rat genotype may be associated with increased insulin binding in the arcuate nucleus. 相似文献
5.
Seed Germination Biology of the Narrowly Endemic Species Lesquerella stonensis (Brassicaceae) 总被引:1,自引:0,他引:1
Abstract Lesquerella stonensis (Brassicaceae) is an obligate winter annual endemic to a small portion of Rutherford County in the Central Basin of Tennessee, where it grows in disturbed habitats. This species forms a persistent seed bank, and seeds remain viable in the soil for at least 6 years. Seeds are dormant at maturity in May and are dispersed as soon as they ripen. Some of the seeds produced in the current year, as well as some of those in the persistent seed bank, afterripen during late spring and summer; others do not afterripen and thus remain dormant. Seeds require actual or simulated spring/summer temperatures to come out of dormancy. Germination occurs in September and October. Fully afterripened seeds germinate over a wide range of thermoperiods (15/6–35/20°C) and to a much higher percentage in light (14 h photoperiod) than in darkness. The optimum daily thermoperiod for germination was 30/15°C. Nondormant seeds that do not germinate in autumn are induced back into dormancy (secondary dormancy) by low temperatures (e.g., 5°C) during winter, and those that are dormant do not afterripen; thus seeds cannot germinate in spring. These seed dormancy/ germination characteristics of L. stonensis do not differ from those reported for some geographically widespread, weedy species of winter annuals and thus do not help account for the narrow endemism of this species. 相似文献
6.
T. I. Baskin 《Planta》1986,169(3):406-414
First positive phototropism of the third internode of intact, 5-d-old pea (Pisum sativum L.) seedlings, grown under continuous, dim red light, showed maximal response following a photon fluence of 3 mol·m-2 blue light. Greater or lesser fluences (with irradiation time 100 s or less) caused less bending, no response being detectable above 300 or below 0.03 mol·m-2. Bilateral irradiation with blue light caused no detectable inhibition of growth rate over that range of fluences. The linear nutation of the pea third internode was shown to be driven by a balanced oscillation of growth rate such that the overall growth rate was little changed during the oscillation. Phototropic stimulation changed neither the amplitude nor the period of nutation. Nutation and phototropism probably regulate growth independently. Phototropism in response to the optimal blue light fluence was caused by concomitant depressed growth on the irradiated side and stimulated growth on the shaded side of the bending internode. These results are consistent with the Cholodny-Went hypothesis which states that unilateral blue light induces a lateral redistribution of a growth regulator.Abbreviations R
red light
- BL
blue light
Carnegie Institution, Department of Plant Biology paper No. 921 相似文献
7.
Baskin DG 《Tissue & cell》1971,3(4):579-587
Fluorescence and electron microscopy reveal a dynamic architectural pattern in the organization of neuroglia in the central nervous system of nereid polychaetes. Fibrous glial processes intertwine among neuronal elements, binding them together, and anchor the nerve cord to the epidermis. Rigidity and resilience are provided to this supportive framework by glial filaments and desmosomes. Tensive stresses, arising from contraction of locomotory muscles attached to the nerve cord, may be dissipated over the web-like meshwork, thus reducing the potential influence of deformative forces on neuronal elements within the neuropile. The voluminous glial processes forming the peripheral zone of the nerve cord may protect neurons from compressive forces. 相似文献
8.
Cell and Tissue Research - The infracerebral complex consists of: (a) two types of ependymoid infracerebral cells located on the ventral surface of the brain, adjacent to a coelomic sinus and blood... 相似文献
9.
D H Mattson N Shimojo E P Cowan J J Baskin R V Turner B D Shvetsky J E Coligan W L Maloy W E Biddison 《Journal of immunology (Baltimore, Md. : 1950)》1989,143(4):1101-1107
Crystallographic studies of the HLA-A2 molecule have led to the assignment of a putative peptide binding site that consists of a groove with a beta-pleated sheet floor bordered by two alpha-helices. A CTL-defined variant of HLA-A2, termed HLA-A2.2F, differs from the common A2.1 molecule by three amino acids: a Leu to Trp substitution at position 156 in the alpha-2 helix, a Val to Leu substitution at position 95 in the beta-sheet floor of the groove, and a Gln to Arg substitution at position 43 in a loop outside of the groove. Another HLA-A2 variant, termed CLA, has a single Phe to Tyr substitution at position 9 that is sterically located adjacent to position 95 in the beta-sheet floor of the groove. We have determined which of the amino acid substitutions at positions 9, 43, 95, or 156 could individually affect recognition by panels of A2.1 allospecific and A2.1-restricted influenza viral matrix peptide-specific CTL lines, using a panel of site-directed mutants and CLA. Recognition by allospecific CTL lines was generally unaffected by any one of the amino acid substitutions, but was eliminated by the double substitution at positions 95 and 156. Allorecognition by some CTL lines was eliminated by a single substitution at position 9 or 95. In contrast, recognition by A2.1-restricted matrix peptide specific CTL was totally eliminated by a single substitution at position 9 or 156. The substitution at position 43 in a loop away from the peptide binding groove had no effect on allorecognition or matrix peptide recognition. These results indicate that amino acid residues in the floor or alpha-2 helical wall of the peptide binding groove of the HLA-A2 molecule can differentially affect allorecognition and viral peptide recognition. 相似文献
10.
Summary The orientation of cortical microtubules in plant cells has been extensively studied, in part because of their influence on the expansion of most plant cell types. Cortical microtubules are often arranged in helical arrays, which are well known to occur with a specific pitch as a function of development or experimental treatment; however, it is not known if the handedness of helical arrays can also be specified. We have studied the handedness of helical arrays by using Vibratome sectioning of maize primary roots and confocal microscopy of Arabidopsis primary roots. In cortical cells of maize roots, the helical array was found to have the same handedness at a given position, not only for the cells of a single root, but also for the cells of more than one hundred roots examined. Quantification of angular distribution of apparent individual microtubules showed that defined regions of the root were composed of cells with highly uniform microtubule orientation. In the region between transverse and longitudinal microtubules (5–10.5 mm from the tip), the array formed a right-handed helix, and basal of cells with longitudinal microtubules (11.5–15 mm from the tip), the array formed a left-handed helix. Similarly, in epidermal cells of Arabidopsis roots right-handed helical arrays were found in the region between transverse and longitudinal microtubules. These results suggest that, in addition to the orientation of microtubules, the handedness of helical microtubule arrays is under cellular control.Abbreviations Cy3
indocarbocyanine
- PBS
phosphate-buffered saline
- PIPES
piperazine-N,N-bis-[2-ethanesulfonic acid] 相似文献