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1.
Barbora Straková Michail Rovatsos Lukáš Kubička Lukáš Kratochvíl 《BioEssays : news and reviews in molecular, cellular and developmental biology》2020,42(10):2000050
Frequent independent origins of environmental sex determination (ESD) are assumed within amniotes. However, the phylogenetic distribution of sex-determining modes suggests that ESD is likely very ancient and may be homologous across ESD groups. Sex chromosomes are demonstrated to be old and stable in endothermic (mammals and birds) and many ectothermic (non-avian reptiles) lineages, but they are mostly non-homologous between individual amniote lineages. The phylogenetic pattern may be explained by ancestral ESD with multiple transitions to later evolutionary stable genotypic sex determination. It is pointed out here that amniote ESD shares several key aspects with sequential hermaphroditism of fishes such as a lack of sex differences in genomes, biased population sex ratios, and potentially also molecular mechanism related to general stress responses. Here, it is speculated that ESD evolves via a heterochronic shift of the sensitive period of sex change from the adult to the embryonic stage in a hermaphroditic amniote ancestor. Also see the video abstract here https://youtu.be/q2mjtlCefu4 . 相似文献
2.
SUMMARY.
- 1 Biomass, production and life history of Mysis relicta were studied in the large Lake Mjøsa during the years 1976–80. Biomass fluctuations were large, but biomass averaged about 1 g wet wt m?2 or 200 ind. m?2. Cumulative net production during summer ranged from 1.6 to 2.1 g wet wt m?2. Mean population P/B-ratio was close to 2.
- 2 Neither production or biomass of Mysis were correlated with the biomass of phytoplankton or the main Zooplankton prey species (Daphnia and Bosmina), suggesting a predominant ‘top down’ control of Mysis in this lake.
- 3 The life cycle was well synchronized with development of food items, but predation pressure also affected life-history patterns. The proportion of juveniles apparently increased in years with strong fish predation pressure, producing a predominantly 1-year life-cycle pattern the following year.
- 4 Mysis had a predominant 2-year life history, but a traction of the population may reproduce after 1 or 3 years. Reproduction occurred exclusively during winter. Even in such a large and stable system, where Mysis has coexisted with its main food items and predators for 8–10 000 years, a flexible life history is maintained and is probably an important buffer against year-to-year fluctuations in food and predator abundance.
3.
4.
The turnover measurement of proteins and proteoforms has been largely facilitated by workflows coupling metabolic labeling with mass spectrometry (MS), including dynamic stable isotope labeling by amino acids in cell culture (dynamic SILAC) or pulsed SILAC (pSILAC). Very recent studies including ours have integrated themeasurement of post-translational modifications (PTMs) at the proteome level (i.e., phosphoproteomics) with pSILAC experiments in steady state systems, exploring the link between PTMs and turnover at the proteome-scale. An open question in the field is how to exactly interpret these complex datasets in a biological perspective. Here, we present a novel pSILAC phosphoproteomic dataset which was obtained during a dynamic process of cell starvation using data-independent acquisition MS (DIA-MS). To provide an unbiased “hypothesis-free” analysis framework, we developed a strategy to interrogate how phosphorylation dynamically impacts protein turnover across the time series data. With this strategy, we discovered a complex relationship between phosphorylation and protein turnover that was previously underexplored. Our results further revealed a link between phosphorylation stoichiometry with the turnover of phosphorylated peptidoforms. Moreover, our results suggested that phosphoproteomic turnover diversity cannot directly explain the abundance regulation of phosphorylation during cell starvation, underscoring the importance of future studies addressing PTM site-resolved protein turnover. 相似文献
5.
Extremophiles - α-Amylase catalyzes the endohydrolysis of α-1,4-glucosidic linkages in starch and related α-glucans. In the CAZy database, most α-amylases have been classified... 相似文献
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7.
Kalocayova Barbora Snurikova Denisa Vlkovicova Jana Navarova-Stara Veronika Michalikova Dominika Ujhazy Eduard Gasparova Zdenka Vrbjar Norbert 《Molecular and cellular biochemistry》2021,476(12):4323-4330
Molecular and Cellular Biochemistry - Previously it was shown that for reduction of anxiety and stress of experimental animals, preventive handling seems to be one of the most effective methods.... 相似文献
8.
Elena V. Buzan Josef Bryja Barbora Zemanová Boris Kryštufek 《Conservation Genetics》2013,14(2):401-412
The chamois is a habitat specialist ungulate occupying “continental archipelagos” of fragmented rocky habitats which are frequently restricted to high altitudes. It is not clear whether forest habitats separating such population fragments act as barriers to gene flow. We studied the genetic makeup of the chamois in a topographically diverse landscape at the contact zone of two mountain ranges in Slovenia. Based on sequences of mitochondrial DNA, all Slovenian populations belong to a Northern chamois (Rupicapra r. rupicapra) subspecies. The range of chamois in Slovenia encompasses three different regions, each with unique topography, habitat connectivity and abundance of chamois: the Alps, the Dinaric Mts., and the Pohorje Mts. The habitat of the chamois is extensive and more or less continuous in the Alps, but suboptimal and fragmented in the remaining regions. In agreement with neutral genetic theory, large Northern chamois populations tended to have higher allelic richness and observed heterozygosity. Spatial clustering bears the differentiation into four geographically associated clusters within Slovenia and also revealed a strong substructure within all mountain ranges with suboptimal chamois habitat. Surprisingly, some small Dinaric populations have stayed genetically isolated in restricted habitat patches, even if they are geographically very close to each other. The four clusters, each having a unique demographic history, should be regarded as independent units for management purposes. 相似文献
9.
Marian Kacerovsky Peter Celec Barbora Vlkova Kristin Skogstrand David M. Hougaard Teresa Cobo Bo Jacobsson 《PloS one》2013,8(3)
Objective
This study aimed to evaluate the amniotic fluid protein profiles and the intensity of intraamniotic inflammatory response to Ureaplasma spp. and other bacteria, using the multiplex xMAP technology.Methods
A retrospective cohort study was undertaken in the Department of Obstetrics and Gynecology, University Hospital Hradec Kralove, Czech Republic. A total of 145 pregnant women with preterm prelabor rupture of membranes between gestational age 24+0 and 36+6 weeks were included in the study. Amniocenteses were performed. The presence of Ureaplasma spp. and other bacteria was evaluated using 16S rRNA gene sequencing. The levels of specific proteins were determined using multiplex xMAP technology.Results
The presence of Ureaplasma spp. and other bacteria in the amniotic fluid was associated with increased levels of interleukin (IL)-6, IL-8, IL-10, brain-derived neurotropic factor, granulocyte macrophage colony stimulating factor, monocyte chemotactic protein-1, macrophage inflammatory protein-1, and matrix metalloproteinasis-9. Ureaplasma spp. were also associated with increased levels of neurotropin-3 and triggering receptor expressed on myeloid cells-1.Conclusions
The presence of Ureaplasma spp. in the amniotic fluid is associated with a slightly different protein profile of inflammatory response, but the intensity of inflammatory response to Ureaplasma spp. is comparable with the inflammatory response to other bacteria. 相似文献10.