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排序方式: 共有497条查询结果,搜索用时 1 毫秒
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A clone coding for Schizophyllum commune beta-glucosidase: homology with a yeast beta-glucosidase 总被引:3,自引:0,他引:3
F Moranelli J R Barbier M J Dove R M MacKay V L Seligy M Yaguchi G E Willick 《Biochemistry international》1986,12(6):905-912
Three identical clones coding for a partial sequence of the Schizophyllum commune beta-glucosidase were isolated from a cDNA library in lambda gt11, using polyclonal antibody to the enzyme. The identity was confirmed by comparison of the amino-terminus of a peptide from a protease lys-C digest with the sequence inferred from the cDNA sequence. A comparison of the sequence with that reported for a beta-glucosidase from Candida pelliculosa revealed a region in the latter with 43% identity in amino acid sequence. There was also a similarity in the S. commune beta-glucosidase to an active site sequence proposed for a S. commune endoglucanase, suggesting the possibility of a common catalytic mechanism for these two glucolytic enzymes. 相似文献
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P Valentin-Hansen J E Larsen P H?jrup S A Short C S Barbier 《Nucleic acids research》1986,14(5):2215-2228
We have determined the nucleotide sequence of the cytR gene, which codes for the Cyt repressor (CytR). The coding region consists of 1023 or 1029 bp. The subunits of CytR are thus predicted to consist of 341 or 343 residues. It is shown that the N-terminal segment of the polypeptide is structurally similar to the DNA-binding region of known DNA-binding proteins. In addition, there exists an exceptionally high amino acid sequence homology between CytR and the Gal repressor, indicating a common origin of evolution. 相似文献
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Seligy VL Barbier JR Dimock KD Dove MJ Moranelli F Morosoli R Willick GE Yaguchi M 《Biotechnology advances》1984,2(2):201-216
New gene selection techniques (Recombinant DNA) are currently available to exploit useful properties of various biological systems hitherto regarded as interesting but of little or no immediate commercial value. The application of genetic engineering techniques to problems in the Pulp and Paper Industry are many. As a first step these techniques are being used to provide much needed fundamental information on the cellular and molecular mechanisms involved in the expression of extra-cellular enzymes that degrade lignocellulosic pulping wastes. The information gleaned from the studies on cellulolytic fungi and bacteria can be used to genetically engineer a yeast or bacterium capable of converting pulping wastes into ethanol and other useful by-products. 相似文献
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β-Sitosterol 1 is metabolised to cholesterol 5 by phytophagous insects. It has been previously shown that fucosterol-24,28 epoxide 3 is transformed into 5 in Locusta migratoria, desmosterol 4 being an intermediate. It is now established that locusts transform [3-3H] fucosterol propionate into the corresponding labelled epoxide 3, recovered as such or as an oxazoline derivative 11. 相似文献
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J.L. Boutry M. Barbier M. Ricard 《Journal of experimental marine biology and ecology》1976,21(1):69-74
12-day cultures of the diatom Chaetoceros simplex calcitrans Paulsen in sea water have been analysed under different conditions of light. With a photophase the primary production is 106 % higher than under continuous light but the unsaponifiable fraction is lower (?42 %) and the sterols increase by 100 %. When ultra-violet irradiation is added to the photophase the primary production is lowered (?56 %) but the unsaponifiable fraction increases by 191 %, and the sterols by 110 %. When ultra-violet irradiation is added to the continuous light there is an increase in primary production (+30 %) and a decrease in the unsaponifiable fraction (?16%).Modifications of the sterol composition are reported. C26 sterols have never been detected in these experiments. 相似文献