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Effect of light on the organogenic ability of garlic roots using a one-step in vitro system 总被引:3,自引:0,他引:3
A simple and efficient garlic in vitro shoot regeneration protocol has been developed. This system uses axenic root tips cultivated from the beginning in the presence of light and does not require any change or refreshing of the original medium during the entire process. The application of light from the beginning of the culture process did not affect the callus formation rate but did significantly improve the explant regeneration ability. In a 2-month period it was possible to obtain up to 250 shoots per gram of callus.Abbreviations
BAP:
6-Benzylaminopurine
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2,4-D:
2,4-Dichlorophenoxyacetic acid
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NAA:
-Naphthaleneacetic acid
Communicated by L. Peña 相似文献
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Since the pioneering work by Julius Adler in the 1960's, bacterial chemotaxis has been predominantly studied as metabolism-independent. All available simulation models of bacterial chemotaxis endorse this assumption. Recent studies have shown, however, that many metabolism-dependent chemotactic patterns occur in bacteria. We hereby present the simplest artificial protocell model capable of performing metabolism-based chemotaxis. The model serves as a proof of concept to show how even the simplest metabolism can sustain chemotactic patterns of varying sophistication. It also reproduces a set of phenomena that have recently attracted attention on bacterial chemotaxis and provides insights about alternative mechanisms that could instantiate them. We conclude that relaxing the metabolism-independent assumption provides important theoretical advances, forces us to rethink some established pre-conceptions and may help us better understand unexplored and poorly understood aspects of bacterial chemotaxis. 相似文献
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正常人肝脏星形细胞的分离,培养及鉴定 总被引:4,自引:0,他引:4
参考Friedman等分离人肝脏星形细胞(HSC)的方法,采用校正密度的淋巴细胞分离液进行一步梯度离心,成功地分离得到了正常人HSC。HSC的收获量约为1×10~7个/10克肝脏组织,存活率在95%以上,纯度达85%以上。传代后纯度接近100%。对人HSC的标志物进行检测发现,结蛋白不宜作为鉴定初分离的及原代培养初期人HSC纯度的指标,而α-平滑肌肌动蛋白可作为鉴定激活的人HSC的可靠指标。 相似文献
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Xabier Barandiaran Nieves Martín Carmen Alba María Fernanda Rodríguez-Conde Antonio Di Pietro Jesus Martín 《In vitro cellular & developmental biology. Plant》1999,35(6):466-469
Summary A simple protocol for garlic in vitro management is described. It comprises a single medium for all developmental stages and
genotypes tested, and the use of immature bulbs as source of axillary buds. Although genetic variability exists among the
different accessions tested, for both the multiplication rate and bulblet size, acceptable values of multiplication were reached
for all the accessions, and values of bulb formation approached 100% in the shoots produced. 相似文献
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Winter Marina Marfil María Jimena La Sala Luciano Francisco Suarez Marcos Maidana Celia Rodriguez Carlos Mesplet María Abate Sergio Rosas Carolina Peña Martinez Jorge Barandiaran Soledad 《EcoHealth》2022,19(2):159-163
Swine coronaviruses affecting pigs have been studied sporadically in wildlife. In Argentina, epidemiological surveillance of TGEV/PRCV is conducted only in domestic pigs. The aim was to assess the prevalence of TGEV/PRCV in wild Suina. Antibodies against these diseases in wild boar and captive collared peccary were surveyed by ELISA. Antibodies against TGEV were found in three collared peccaries (n?=?87). No TGEV/PRCV antibodies were detected in wild boar (n?=?160). Preventive measures should be conducted in contact nodes where the transmission of agents may increase. Epidemiological surveillance in wildlife populations and in captive animals before their reintroduction should be attempted.
相似文献8.
Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L. 总被引:3,自引:0,他引:3
The biolistic particle delivery system was used to transfer the uidA gene into different garlic tissues, including regenerable calli. These tissues showed a high endogenous nuclease activity
preventing exogenous DNA expression. After submerging the tissues for 24 h in a 2 mm solution of the nuclease inhibitor aurintricarboxylic acid, transient expression of the uidA gene was detected. Furthermore, vacuum infiltration of aurintricarboxylic acid into the garlic tissues significantly increased
the percentage of explants showing transient expression. Among four plasmids tested, pDE4 containing the CaMV35S-uidA construct produced the best results.
Received: 16 May 1997 / Revision received: 26 August 1997 / Accepted: 25 September 1997 相似文献
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X. Barandiaran N. Martín M. F. Rodríguez-Conde A. Di Pietro J. Martín 《Plant cell reports》1999,18(5):434-437
Twenty garlic Allium sativum (L.) genotypes were analysed for genetic variation in their ability to form callus (in one medium) and regenerate shoots
(in four different media). Genotypes showed significant differences in the analysis of variance of all the traits tested.
The accession Printanor showed the best general behaviour, with 83% callus-producing explants, 44.7% organogenic explants,
and 15.35 shoots/g of callus. The best regeneration medium was MBO, without growth regulators. Shoot production capacity was
examined with the additive main effects and multiplicative interaction model that proved to be a powerful tool for analysis
and easy comprehension of the strong genotype×medium interactions frequently observed in in vitro culture systems.
Received: 5 February 1998 / Revision received: 11 May 1998 / Accepted: 1 June 1998 相似文献
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