Influence of dissolved organic matter from terrestrial origin on the changes of dinoflagellate species composition in the Gulf of Riga,Baltic Sea

Hydrobiologia - A mesocosm experiment was used to investigate the effect of terrestrial-origin dissolved organic matter (DOM) on the development of dinoflagellates in natural summer phytoplankton...     

Symmetric molecules with 1,4-triazole moieties as potent inhibitors of tumour-associated lactate dehydrogenase-A

A series of symmetric molecules incorporating aryl or pyridyl moieties as central core and 1,4-substituted triazoles as a side bridge was synthesised. The new compounds were investigated as lactate dehydro-genase (LDH, EC 1.1.1.27) inhibitors. The cancer associated LDHA isoform was inhibited with IC₅₀?=?117–174 µM. Seven compounds exhibited better LDHA inhibition (IC₅₀ 117–136 µM) compared to known LDH inhibitor – galloflavin (IC₅₀ 157 µM).     

Effects of nutrient enrichment on the growth rates and community structure of summer phytoplankton from the Gulf of Riga, Baltic Sea

Sea water collected in mid-July was spiked with different combinationsof nutrients. Dissolved inorganic nitrogen (DIN) became exhausted,while significant amounts of P-PO₄ and Si-SiO₃ remained available.NH₄ was taken up before NO₃, but, in some cultures, NO₃ uptakeappears to have started in the presence of     

Insight into the studies of the practical use of microalgae in the former Soviet Union

Microalgae have been intensively cultivated and used in the national economy of the Soviet Union in Russia (Moscow, Leningrad, Pushcino, Krasnoyarsk, Irkutsk), the Ukraine, Byelorussia (Minsk), Azerbaijan (Baku), Uzbekistan (Tashkent), Latvia (Riga) and other former Soviet republics. The first studies on the practical use of microalgae started already at the beginning of last century and were connected with treatment of waste water in biological ponds. Ecophysiological studies of marine plankton algae in mono- and mixed cultures as well as in experimental systems with zooplankton organisms started at the end of the 1950s. In the 1960s, started to apply different equipments for cultivation of microalgae in open water basins and closed ecological systems. Research innovations were introduced in agriculture. The productivity of aquatic ecosystems and the distribution of energy within food chains were studied. Intensive research on microalgae continued, paying attention to the chemical composition, physiological and biochemical features. Nutrient supply appeared as one of the main problem for productivity of algal cultures, along with the procession, biochemistry of nutrients and nutrition value of organic matter produced by algae. The most attention was paid to the Chroococcales proteins and possibilities for their use in man-made closed ecological systems. The potential use of microalgae for regeneration of the atmosphere and purification of water in spacecrafts was studied. During the 1970s attention was paid to the role of algae in self-purification processes and biodegradation of surface-active substances in water bodies. Research in microalgae as bioindicators for the assessment of environment quality of aquatic ecosystems and as test objects for biotesting of natural and waste waters was conducted from the 1970s to 1980s.     

Evaluation of a new semi-automated Hydragel 11 von Willebrand factor multimers assay kit for routine use

BackgroundAccurate diagnosis and classification of von Willebrand disease (VWD) are essential for optimal management. The von Willebrand factor multimers analysis (VWF:MM) is an integral part of the diagnostic process in the phenotypic classification, especially in discrepant cases. The aim of this study was to evaluate the performance of a new Hydragel 11VWF multimer assay (H11VW).MethodsAnalytical performance characteristics such as repeatability (intra-assay variability, in gel between track variation), reproducibility (inter-assay variability, between gel variation), sensitivity, EQA performance and differences between two commercially available VWF:MM kits (H5VW and H11VW) were analysed in healthy volunteers'' plasmas using in-house prepared reference plasma.ResultsRepeatability and reproducibility results of H11VW demonstrated acceptable and equivalent performance with previously verified H5VW. Participation in EQA was successful. No statistically significant difference was detected between H5VW and H11VW kits for different fractions of multimers: LMWM p=0.807; IMWM p=0.183; HMWM p=0.774.ConclusionsH11VW demonstrated acceptable analytical performance characteristics. H11VW kit conveniently offers a more significant number of samples on a single gel. H5VW and H11VW kits can be used in daily practice interchangeably.     

The use of spectral fluorescence methods to detect changes in the phytoplankton community

In vivo fluorescence methods are efficient toolsfor studying the seasonal and spatial dynamics ofphytoplankton. Traditionally the measurements are madeusing single excitation-emission wavelengthcombination. During a cruise in the Gulf of Riga(Baltic Sea) we supplemented this technique bymeasuring the spectral fluorescence signal (SFS) andfixed wavelength fluorescence intensities at theexcitation maxima of main accessory pigments. Thesemethods allowed the rapid collection of quantitativefluorescence data and chemotaxonomic diagnostics ofthe phytoplankton community. The chlorophylla-specific fluorescence intensities (R) and thespectral fluorescence fingerprints were analysedtogether with concentrations of chlorophyll a indifferent algal size-groups, phytoplankton biomass andtaxonomic position. The lower level of R in thesouthern gulf was related to the higher proportion ofcyanobacteria relative to total biomass and the lowerabundance of small algae. The phycoerythrinfluorescence signal was obviously due to the largecyanobacteria. The basin-wide shift in the shape ofchlorophyll a excitation spectra was caused bythe variable proportions of differently pigmentedcyanobacteria, diatoms and cryptomonads. This revised version was published online in July 2006 with corrections to the Cover Date.