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M. C. Nardi E. Giacomelli P. Dainese A.-C. Fitchette-Lain L. Faye B. Baldan L. Navazio P. Mariani 《Plant biology (Stuttgart, Germany)》1998,111(1):66-70
Calreticulin, the main Ca2+ binding protein in the endoplasmic reticulum of eukaryotic cells, was characterized in Ginkgo biloba L. pollen and seeds. Electrophoretic analysis of the partly purified extracts showed the presence of two protein bands of 57 and 50kDa apparent molecular masses, which strongly cross-reacted with antibodies against plant calreticulins. Amino acid sequence comparison with other plant and animal calreticulins revealed a much higher similarity of the N-terminus of Ginkgo calreticulins with the homologue from angiosperms rather than with that from mammals. The finding of calreticulin in Ginkgo is indicative of the conservation also in gymnosperms of Ca2+ homeostatic mechanisms, which seem to rely on the same molecular components as all eukaryotic cells. 相似文献
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Enrico Baldan Sebastiano Nigris Chiara Romualdi Stefano D’Alessandro Anna Clocchiatti Michela Zottini Piergiorgio Stevanato Andrea Squartini Barbara Baldan 《PloS one》2015,10(10)
We investigated the potential plant growth-promoting traits of 377 culturable endophytic bacteria, isolated from Vitis vinifera cv. Glera, as good biofertilizer candidates in vineyard management. Endophyte ability in promoting plant growth was assessed in vitro by testing ammonia production, phosphate solubilization, indole-3-acetic acid (IAA) and IAA-like molecule biosynthesis, siderophore and lytic enzyme secretion. Many of the isolates were able to mobilize phosphate (33%), release ammonium (39%), secrete siderophores (38%) and a limited part of them synthetized IAA and IAA-like molecules (5%). Effects of each of the 377 grapevine beneficial bacteria on Arabidopsis thaliana root development were also analyzed to discern plant growth-promoting abilities (PGP) of the different strains, that often exhibit more than one PGP trait. A supervised model-based clustering analysis highlighted six different classes of PGP effects on root architecture. A. thaliana DR5::GUS plantlets, inoculated with IAA-producing endophytes, resulted in altered root growth and enhanced auxin response. Overall, the results indicate that the Glera PGP endospheric culturable microbiome could contribute, by structural root changes, to obtain water and nutrients increasing plant adaptation and survival. From the complete cultivable collection, twelve promising endophytes mainly belonging to the Bacillus but also to Micrococcus and Pantoea genera, were selected for further investigations in the grapevine host plants towards future application in sustainable management of vineyards. 相似文献
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Lorella Navazio Barbara Baldan Roberto Moscatiello Anna Zuppini Sheridan L Woo Paola Mariani Matteo Lorito 《BMC plant biology》2007,7(1):41
Background
Calcium is commonly involved as intracellular messenger in the transduction by plants of a wide range of biotic stimuli, including signals from pathogenic and symbiotic fungi. Trichoderma spp. are largely used in the biological control of plant diseases caused by fungal phytopathogens and are able to colonize plant roots. Early molecular events underlying their association with plants are relatively unknown. 相似文献5.
An anthocyanin-producing suspension culture of Daucus carota (L.) cv. Flakkese was used as model system to study secondary metabolite production in cell culture at the individual cell level. An approach was set up in which growth and production of anthocyanins were investigated using a combination of biochemical analysis, image (colour) analysis and in vivo imaging. This novel approach was used to segment the culture in different subpopulations and dissect the productive process in the cell culture grown under two different conditions, known to differ mainly for oxygen supply and mixing intensity (volume of 50 ml or 20 ml in 250 ml flasks). The 20 ml batch cultures gave a higher content and yield of anthocyanins, which depended on a complex balance between events that positively or negatively affected anthocyanin production. A model is proposed in which the different ability of cells to respond to environmental stimuli and stress depends on the different amount of anthocyanins accumulated within cells. 相似文献
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Coexistence of predominantly nonculturable rhizobia with diverse, endophytic bacterial taxa within nodules of wild legumes 总被引:1,自引:0,他引:1
Muresu R Polone E Sulas L Baldan B Tondello A Delogu G Cappuccinelli P Alberghini S Benhizia Y Benhizia H Benguedouar A Mori B Calamassi R Dazzo FB Squartini A 《FEMS microbiology ecology》2008,63(3):383-400
A previous analysis showed that Gammaproteobacteria could be the sole recoverable bacteria from surface-sterilized nodules of three wild species of Hedysarum. In this study we extended the analysis to eight Mediterranean native, uninoculated legumes never previously investigated regarding their root-nodule microsymbionts. The structural organization of the nodules was studied by light and electron microscopy, and their bacterial occupants were assessed by combined cultural and molecular approaches. On examination of 100 field-collected nodules, culturable isolates of rhizobia were hardly ever found, whereas over 24 other bacterial taxa were isolated from nodules. None of these nonrhizobial isolates could nodulate the original host when reinoculated in gnotobiotic culture. Despite the inability to culture rhizobial endosymbionts from within the nodules using standard culture media, a direct 16S rRNA gene PCR analysis revealed that most of these nodules contained rhizobia as the predominant population. The presence of nodular endophytes colocalized with rhizobia was verified by immunofluorescence microscopy of nodule sections using an Enterobacter-specific antibody. Hypotheses to explain the nonculturability of rhizobia are presented, and pertinent literature on legume endophytes is discussed. 相似文献
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Asma Torche Hayet Benhizia Riccardo Rosselli Ottavia Romoli Marina Zanardo Enrico Baldan Sara Alberghini Alessandra Tondello Barbara Baldan Amar Benguedouar Andrea Squartini Yacine Benhizia 《Annals of microbiology》2014,64(3):1065-1071
The root nodules of two wild legume species endemic to Algeria, Hedysarum naudinianum and He. perrauderianum, were investigated with regard to their anatomy and histology, and the identity of the associated bacteria. Both plants were found to form root nodules with regular features and well infected by rod-shaped bacteria. The culturable fraction of bacteria that could be obtained from surface-sterilized nodules included a prevailing presence of Enterobacteriaceae having 100 % 16S rDNA sequence identity with both Enterobacter cloacae and E. ludwigii. In H. perrauderianum, this taxon was the sole cultured isolate, while from H. naudinianum we also found Bacillus, Lactobacillus, Staphylococcus, Rothia, and isolates that were 100 % identical to Corynebacterium pseudodiphthericum, which is known to be an agent of respiratory and cardiac infections in humans. Whereas no culturable rhizobia and alike could be obtained on plates, PCR-based culture-independent approaches revealed in both plants the presence of a Mesorhizobium sp., which in H. perrauderianum was identical to isolates nodulating other legumes from Africa, European Mediterranean countries, and Asia, while in H. naudinianum it bore a single nucleotide polymorphism which is so far unique for any observed mesorhizobia. Data from the microsymbionts appear to suggest interesting clues to interpret the evolutionary ecology of their host plants. 相似文献
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A protocol for obtaining embryogenic cell lines from Arabidopsis 总被引:1,自引:1,他引:0
Elisa Pillon Mario Terzi Barbara Baldan Paola Mariani Fiorella Lo Schiavo 《The Plant journal : for cell and molecular biology》1996,9(4):573-577
Embryogenic cell lines with lasting embryogenic potential can be obtained from somatic embryos induced directly from zygotic embryos of Arabidopsis thaliana , ecotype Columbia. The response to a critical concentration of auxin, which seems to be the all-important factor in the generation of embryogenic cell lines, is exhibited by somatic embryos but not by zygotic ones. The basis for this differential response remains obscure and will be discussed in relation to other systems. 相似文献
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Flavia Guzzo Barbara Baldan Marisa Levi E. Sparvoli Fiorella Lo Schiavo M. Terzi Paola Mariani 《Protoplasma》1995,185(1-2):28-36
Summary The cellular events occurring in carrot hypocotyl explants during long-term and pulse treatment with 2,4-D were followed using different techniques (light and transmission electron microscopy, flow cytometry, PCNA staining). Different morphogenetic pathways were induced under the various experimental conditions. Nevertheless, in the explants the activated cells were the same (provascular cells) and they showed very similar structural and ultrastructural changes. The long-term treatment with 2,4-D induced rapid re-activation of the cell cycle.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- DAPI
4,6-diamidino-2-phenylindole
- PCNA
proliferating cell nuclear antigen
- ER
endoplasmic reticulum
- GA
Golgi apparatus 相似文献