全文获取类型
收费全文 | 133篇 |
免费 | 9篇 |
出版年
2021年 | 1篇 |
2019年 | 1篇 |
2017年 | 1篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 4篇 |
2012年 | 4篇 |
2011年 | 3篇 |
2010年 | 5篇 |
2009年 | 7篇 |
2008年 | 1篇 |
2006年 | 2篇 |
2005年 | 1篇 |
2003年 | 4篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 7篇 |
1998年 | 5篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 3篇 |
1990年 | 1篇 |
1989年 | 5篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 6篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 7篇 |
1980年 | 2篇 |
1979年 | 10篇 |
1978年 | 12篇 |
1977年 | 4篇 |
1975年 | 1篇 |
1974年 | 5篇 |
1973年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有142条查询结果,搜索用时 812 毫秒
1.
A non-chemical means of preventing premature pole failure in soil-contact was investigated by applying open-ended cylindrical sleeves of heat-shrink polyethylene to the soil-contact surfaces of Eucalyptus grandis poles as physical barriers to fungal colonisation from soil in sub-tropical South Africa. The test site was managed under flood-irrigation to represent vineyard conditions. After exposure for 26 weeks all untreated unsleeved poles had failed, whereas sleeved poles were colonised by fungi but remained relatively sound. Unsleeved poles treated with 16 kg CCA m−3 were colonised by fungi at their soil-contact surfaces, whereas their sleeved counterparts were uncolonised. Unsleeved poles treated with 100 kg creosote m−3 were extensively covered by biofilm and showed incipient decay at their soil-contact surfaces, whereas their sleeved counterparts were uncolonised. 相似文献
2.
3.
4.
5.
6.
7.
C Crone J Frokjaer-Jensen JJ Friedman O Christensen 《The Journal of general physiology》1978,71(2):195-220
8.
9.
Lipoteichoic acid is an important microbe-associated molecular pattern of Lactobacillus rhamnosus GG
Claes Ingmar JJ Segers Marijke E Verhoeven Tine LA Dusselier Michiel Sels Bert F De Keersmaecker Sigrid CJ Vanderleyden Jos Lebeer Sarah 《Microbial cell factories》2012,11(1):1-8
Background
Receptors with a single transmembrane (TM) domain are essential for the signal transduction across the cell membrane. NMR spectroscopy is a powerful tool to study structure of the single TM domain. The expression and purification of a TM domain in Escherichia coli (E.coli) is challenging due to its small molecular weight. Although ketosteroid isomerase (KSI) is a commonly used affinity tag for expression and purification of short peptides, KSI tag needs to be removed with the toxic reagent cyanogen bromide (CNBr).Result
The purification of the TM domain of p75 neurotrophin receptor using a KSI tag with the introduction of a thrombin cleavage site is described herein. The recombinant fusion protein was refolded into micelles and was cleaved with thrombin. Studies showed that purified protein could be used for structural study using NMR spectroscopy.Conclusions
These results provide another strategy for obtaining a single TM domain for structural studies without using toxic chemical digestion or acid to remove the fusion tag. The purified TM domain of p75 neurotrophin receptor will be useful for structural studies. 相似文献10.