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Osmotic priming treatments reduced both the mean time to germinationand the spread of germination for two leek seed-lots of highviability but differing vigour. In addition the differencesin germination performance between these two seed-lots was abolishedby the priming treatments. In the unprimed seed-lots, differencesin germination performance were reflected in differences inrates of protein biosynthesis in leek embryo tissue during germination.Osmopriming treatments abolished these differences upon subsequentgermination of osmotically primed seed and furthermore inducedhigh levels of protein biosynthesis in embryo tissue. DNA synthesiswas detectable in leek embryos during the priming period inthe absence of any cell division and was followed by a five-foldincrease in the rate of DNA synthesis in embryo tissue upongermination following priming at which time the rates of DNAsynthesis in these leek embryos was significantly greater thanthat found at any time over the first 4 d of germination inembryos of unprimed leek seeds. The increases in rates of bothprotein and DNA synthesis observed upon germination of primedseed occurred only after a 612 h lag period during whichtime there is little increase in these rates above those foundat the end of priming Analysis of nucleotide and nucleotide sugar levels in leek embryosboth during and after priming showed that only traces of GTPand CTP and low levels of ATP and UTP were present in embryosduring priming. After a 6 h lag period following the end ofpriming these levels increased sharply, probably via de novosynthesis. A similar pattern was found for UDP glucose levelsduring priming and subsequent germination. These results indicatethat there is considerable biochemical activity during primingand that the significant benefits in germination performanceof primed leek seeds is accompanied by marked increases in protein,DNA and nucleotide biosynthesis after a lag period of 612h following the end of the priming period Allium porrum, leek, seed, osmopriming, germination, protein synthesis, nucleic acids, nucleotides, nucleotide sugars 相似文献
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Martijn F Schenk Jan HG Cordewener Antoine HP America Wendy PC van't Westende Marinus JM Smulders Luud JWJ Gilissen 《BMC plant biology》2009,9(1):24
Background
Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch (Betula pendula) have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus Betula. Q-TOF LC-MSE was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome. 相似文献6.
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Owing to its lipophilic property, carbon tetrachloride (CCl4) is rapidly absorbed by both the liver and brain. We investigated the protective effects of crocin against brain damage caused by CCl4. Fifty rats were divided into five groups of ten: control, corn oil, crocin, CCl4 and CCl4 + crocin. CCl4 administration decreased glutathione (GSH) and total antioxidant status (TAS) levels, and catalase (CAT) activity, while significant increases were observed in malondialdehyde (MDA) and total oxidant status (TOS) levels and superoxide dismutase (SOD) activity. The cerebral cortex nuclear lamina developed a spongy appearance, neuronal degeneration was observed in the hippocampus, and heterochromatic and pyknotic neurons with increased cytoplasmic eosinophilia were observed in the hippocampus after CCl4 treatment. Because crocin exhibits strong antioxidant properties, crocin treatment increased GSH and TAS levels and CAT activities, and decreased MDA and TOS levels and SOD activity; significant improvements also were observed in histologic architecture. We found that crocin administration nearly eliminated CCl4 induced brain damage by preventing oxidative stress. 相似文献
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