Some Aspects of the Reproductive Biology of Limonium vulgare Mill., and Limonium humile Mill.

The germination of two species of salt-marsh plants Limoniumvulgare Mill., and L. humile Mill., is investigated in relationto their ecology. Germination is inhibited by sea water, butgermination even in fresh water is low. Inhibition of germinationby sea water is negatively correlated with water uptake. However,a treatment in sea water stimulates subsequent germination infresh water. The effect of the sea-water treatment appears tobe one of osmotic shock which weakens the seed coat. Variationin response to the treatment is partly genetical and partlyenvironmental. Seeds in different parts of the salt-marsh willbe subject to differing conditions, both during seed developmentand during the interval between seed maturation and seed dispersal.In connection with this the effect of leaching by sea waterand fresh water is considered. The response to a sea-water treatmentvaries with time. Considered in the long term it shows somecyclical variation which might correspond with the tidal cycle.It is shown that seeds of Limonium can survive long periodsin sea water. There is a slow rate of germination during immersion.Most of those seeds which do not germinate in sea water surviveto germinate rapidly in fresh water. The response to oxygenconcentration, light, and temperature is also investigated.Experiments on seedling growth and establishment show the needfor fresh water and a suitable substrate. Mud gives a lowergermination but a higher survival, while sand gives a highergermination but a lower survival. The dispersal of Limoniumseeds in nature is investigated. Seeds of Limonium are mostabundant in the drift line, but even these only represent asmall fraction of the total produced. It is considered thatreproduction by seed is only important when a new area is beinginvaded. Subsequent reproduction is largely by vegetative spread.The ecological implications of the germination experiments arediscussed.     

Sperm (ejaculate) competition in Drosophila melanogaster, and the reproductive value of females to males in relation to female age and mating status

• 1 In double mating experiments with Drosophila melanogaster in which one male had been irradiated, it was confirmed that sperm displacement is extensive, i.e. the second male to mate displaces most of the previously-stored sperm.
• 2 The predominance of the second ejaculate over the first increases with the interval between the two matings, from about P₂= 0.83 (second mating on the first day after the first mating) to about P₂= 0.99 (interval between mating = 14 days) where P₂ is the proportion of offspring fathered by the second male.
• 3 A more accurate method for calculating P₂ values is developed for experiments in which sperm are ‘labelled’ by irradiation treatment (equation 1).
• 4 Observations of the reducing egg production of the female throughout life were also obtained. A model is examined which incorporates both the sperm competition and egg production data to predict the reproductive value to a male of a mating with a given type of female, varying in age and mating status. The relative value (in terms of probable numbers of progeny gained) of a mating with a virgin or 4 day post-mating female is about twice that of a 14 day post-mating female, mainly because of the fecundity difference.
• 5 Some evolutionary aspects of sperm competition and multiple mating in insects are reviewed and discussed.

     

The U.K. Acid Waters Monitoring Network: an assessment of chemistry data, 1988–93

1. The current extent and distribution of acidified surface waters in the U.K. is assessed using data collected between 1988 and 1993 as part of the U.K. Acid Waters Monitoring Network (AWMN). The data have been collected against a background of constant S deposition across the U.K. throughout the monitoring period. 2. Surface waters with mean pH < 5.0 occur in south-west Scotland, south-east Northern Ireland, the Lake District and south-east England. In addition, sites with mean pH < 5.5 are found in central and north-east Scotland, the Pennines and north Wales. These areas receive, and have historically received, high levels of S deposition and xSO₄ is identified as the main contribution to non-marine acid anion concentrations. 3. Mean total oxidized nitrogen (TON) concentrations are > 10 μeq l^–1 at ten of the twenty-two sites which are found in areas broadly corresponding to areas of high N deposition. The mean contribution of TON to total non-marine acid anion concentration is > 10% at sixteen sites and > 20% at six sites. Most sites, except for those with TON < 10 μeq l^–1 and mainly located in north-west Scotland and Northern Ireland, demonstrate strong seasonality in TON concentrations and during autumn and winter instantaneous contributions of TON to total non-marine acid anions is up to 60%. This confirms the importance of N with respect to surface water acidification and strengthens the case for implementation of controls on future N emissions. 4. The data period is currently too short for rigorous trend analysis given the monthly/quarterly sampling frequency in streams/lakes, respectively. Nevertheless, increased SO₄ and xSO₄ can be observed in the time series at sites in south-west Scotland and north Wales despite constant deposition to these areas. Similar trends are evident in longer-term more frequent data at two sites and may reflect hydrometeorological influences, especially the duration and intensity of hot and dry weather in summer which promotes mineralization of organic S.     

Culicoides in relation to transmission of African Horse Sickness virus in The Gambia

Twelve light trap collections made near overnight shelters of horses and donkeys in four villages in the Central River Division of The Gambia captured fourteen species of biting midge of the genus Culicoides . Five species new to The Gambia were identified. This brought the number of recognized species of Culicoides (after a revision of C. schultzei ) to twenty-nine in The Gambia. Species known or suspected as vectors of African horse sickness virus (AHSV) and bluetongue virus (BTV) comprised 83% of female captures, 65% of captures being C. imicola or its sibling species , C. miombo . Captures of female Culicoides in the late dry season were almost as large as in the early dry season, despite the extreme heat and dryness at this time of the year.
Tests on batches of formalin-preserved female midges, using AHSV or BTV antigen capture ELISAs, did not show the presence of any virus amongst 2286 females in 240 aliquots. Nearly all Gambian equines are reportedly seropositive to AHSV and these results suggest that virus challenge from Culicoides vectors may be a factor in the health of Gambian horses and donkeys.     

Induction of salivation in biting midges and mosquitoes, and demonstration of virus in the saliva of infected insects

Culicoides biting midges and Aedes aegypti (Linnaeus) mosquitoes were induced to salivate by the topical application of pilocarpine, neostigmine, malathion and dimethoate; of these, malathion was the most effective. Drops of saliva produced by virus-infected midges and mosquitoes were shown to contain virus. The method could be used to demonstrate transmission in insects infected with a variety of pathogens.     

Light and suction trap catches of Culicoides midges in southern England

1. The seasonal flight periods are given for eleven species or species groups of Culicoides midges at Pirbright, Surrey, U.K., from 1979 to 1981 and in 1983. 2. Most species were relatively uniformly distributed both within the study site and between years; only C.clintoni Boorman was limited in its spatial distribution and only C.segnis Campbell and Pelham-Clinton varied greatly in numbers between years. 3. Species fall into one of three patterns of seasonal abundance: spring emergers, summer emergers, or spring to autumn emergers. 4. Only the C.obsoletus group showed a pattern of emergence that could correspond to distinct generations, of one or more species in the group. Three distinct emergences were observed, and possible explanations for these discussed. 5. For all species except C.impunctatus Goetghebuer the catches from suction/light and suction only traps are poorly correlated whereas for C.impunctatus they are highly correlated. This may suggest that this species is not greatly attracted to light.