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A major question in the analysis of teleost epiboly is the fateof the yolk cytoplasmic layer. It diminishes during epibolyand eventually disappears at the completion of epiboly. Thispaper is concerned with the fate of the surface of the yolkcytoplasmic layer during epiboly. When gastrulae during epibolyare bathed in lucifer yellow (CH) and then observed with fluorescentmicroscopy or bathed in ferritin and then fixed and observedwith TEM, a thin circumferential ring of endocytic vesiclesis observed, confined to the external yolk syncytial layer justperipheral to the advancing margin of the blastoderm. Even thoughthe entire egg is immersed in the marker, endocytosis is confinedto this limited region. More precisely, this endocytosis occursonly within the region of the external yolk syncytial layer,where the surface is most folded. The endocytic vesicles thusformed move downward and settle on the surface of the membraneseparating the yolk from the cytoplasm in the yolk syncytiallayer. They do not join the surface of the internal yolk syncytiallayer; hence they do not contribute to its expansion. Priorto the onset of epiboly there is no such endocytosis at thesurface of the egg. Since this endocytosis occurs only duringepiboly and only at the surface of the external yolk syncytiallayer just peripheral to the advancing margin of the blastoderm,and in the absence of large molecules in the medium, we concludethat it is programmed. We, therefore, present this as a caseof programmed internalization of cell surface serving as themorphogenetic mechanism responsible for the disappearance ofthe surface of the yolk cytoplasmic layer during gastrulationof the teleost Fundulus heteroclitus 相似文献
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