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1.
Herman Kosasih Bachti Alisjahbana Nurhayati Quirijn de Mast Irani F. Rudiman Susana Widjaja Ungke Antonjaya Harli Novriani Nugroho H. Susanto Hadi Jusuf Andre van der Ven Charmagne G. Beckett Patrick J. Blair Timothy H. Burgess Maya Williams Kevin R. Porter 《PLoS neglected tropical diseases》2016,10(2)
Background
Dengue has emerged as one of the most important infectious diseases in the last five decades. Evidence indicates the expansion of dengue virus endemic areas and consequently the exponential increase of dengue virus infections across the subtropics. The clinical manifestations of dengue virus infection include sudden fever, rash, headache, myalgia and in more serious cases, spontaneous bleeding. These manifestations occur in children as well as in adults. Defining the epidemiology of dengue in a given area is critical to understanding the disease and devising effective public health strategies.Methodology/Principal Findings
Here, we report the results from a prospective cohort study of 4380 adults in West Java, Indonesia, from 2000–2004 and 2006–2009. A total of 2167 febrile episodes were documented and dengue virus infections were confirmed by RT-PCR or serology in 268 cases (12.4%). The proportion ranged from 7.6 to 41.8% each year. The overall incidence rate of symptomatic dengue virus infections was 17.3 cases/1,000 person years and between September 2006 and April 2008 asymptomatic infections were 2.6 times more frequent than symptomatic infections. According to the 1997 WHO classification guidelines, there were 210 dengue fever cases, 53 dengue hemorrhagic fever cases (including one dengue shock syndrome case) and five unclassified cases. Evidence for sequential dengue virus infections was seen in six subjects. All four dengue virus serotypes circulated most years. Inapparent dengue virus infections were predominantly associated with DENV-4 infections.Conclusions/Significance
Dengue virus was responsible for a significant percentage of febrile illnesses in an adult population in West Java, Indonesia, and this percentage varied from year to year. The observed incidence rate during the study period was 43 times higher than the reported national or provincial rates during the same time period. A wide range of clinical severity was observed with most infections resulting in asymptomatic disease. The circulation of all four serotypes of dengue virus was observed in most years of the study. 相似文献2.
Jiangbin Zhang Moritz H. Futscher Vincent Lami Felix U. Kosasih Changsoon Cho Qinying Gu Aditya Sadhanala Andrew J. Pearson Bin Kan Giorgio Divitini Xiangjian Wan Dan Credgington Neil C. Greenham Yongsheng Chen Caterina Ducati Bruno Ehrler Yana Vaynzof Richard H. Friend Artem A. Bakulin 《Liver Transplantation》2019,9(47)
Bulk heterojunction (BHJ) nonfullerene organic solar cells prepared from sequentially deposited donor and acceptor layers (sq‐BHJ) have recently been shown to be highly efficient, environmentally friendly, and compatible with large area and roll‐to‐roll fabrication. However, the related photophysics at donor‐acceptor interface and the vertical heterogeneity of donor‐acceptor distribution, critical for exciton dissociation and device performance, have been largely unexplored. Herein, steady‐state and time‐resolved optical and electrical techniques are employed to characterize the interfacial trap states. Correlating with the luminescent efficiency of interfacial states and its nonradiative recombination, interfacial trap states are characterized to be about 40% more populated in the sq‐BHJ devices than the as‐cast BHJ (c‐BHJ), which probably limits the device voltage output. Cross‐sectional energy‐dispersive X‐ray spectroscopy and ultraviolet photoemission spectroscopy depth profiling directly visualize the donor–acceptor vertical stratification with a precision of 1–2 nm. From the proposed “needle” model, the high exciton dissociation efficiency is rationalized. This study highlights the promise of sequential deposition to fabricate efficient solar cells, and points toward improving the voltage output and overall device performance via eliminating interfacial trap states. 相似文献
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Lepa Syahrani Dendi H. Permana Din Syafruddin Siti Zubaidah Puji B. S. Asih Ismail E. Rozi Anggi P. N. Hidayati Sully Kosasih Farahana K. Dewayanti Nia Rachmawati Rifqi Risandi Michael J. Bangs Claus Bgh Jenna Davidson Allison Hendershot Timothy Burton John P. Grieco Nicole L. Achee Neil F. Lobo 《PLoS neglected tropical diseases》2022,16(3)
Mosquitoes are important vectors that transmit pathogens to human and other vertebrates. Each mosquito species has specific ecological requirements and bionomic traits that impact human exposure to mosquito bites, and hence disease transmission and vector control. A study of human biting mosquitoes and their bionomic characteristics was conducted in West Sumba and Southwest Sumba Districts, Nusa Tenggara Timur Province, Indonesia from May 2015 to April 2018. Biweekly human landing catches (HLC) of night biting mosquitoes both indoors and outdoors caught a total of 73,507 mosquito specimens (59.7% non-Anopheles, 40.3% Anopheles). A minimum of 22 Culicinae species belonging to four genera (Aedes, Armigeres, Culex, Mansonia), and 13 Anophelinae species were identified. Culex quinquefasciatus was the dominant Culicinae species, Anopheles aconitus was the principal Anopheles species inland, while An. sundaicus was dominant closer to the coast. The overall human biting rate (HBR) was 10.548 bites per person per night (bpn) indoors and 10.551 bpn outdoors. Mosquitoes biting rates were slightly higher indoors for all genera with the exception of Anopheles, where biting rates were slightly higher outdoors. Diurnal and crepuscular Aedes and Armigeres demonstrated declining biting rates throughout the night while Culex and Anopheles biting rates peaked before midnight and then declined. Both anopheline and non-anopheline populations did not have a significant association with temperature (p = 0.3 and 0.88 respectively), or rainfall (p = 0.13 and 0.57 respectively). The point distribution of HBR and seasonal variables did not have a linear correlation. Data demonstrated similar mosquito–human interactions occurring outdoors and indoors and during early parts of the night implying both indoor and outdoor disease transmission potential in the area–pointing to the need for interventions in both spaces. Integrated vector analysis frameworks may enable better surveillance, monitoring and evaluation strategies for multiple diseases. 相似文献
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Summary Indonesians from North Sumatra were surveyed for erythrocyte enzymes and serum protein variants. The gene frequency of 6PGDC in 264 Batak was 0.051; in small numbers of other racial groups it ranged from 0.016 to 0.060. The gene frequency of PGM
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in 272 Batak was 0.761; in small numbers of other racial groups it ranged from 0.700 to 0.808. PHI type 4-1 was found once in 271 Batak, and PHI 4-1, PHI 3-1, and PHI 6-1 were each found once in 55 Chinese. No variants were found in the MDH and LDH systems. The Hp1 gene frequency in 271 Batak was 0.284; in small numbers of other racial groups it ranged from 0.216 to 0.292. The Tf D gene frequency in 262 Batak was 0.044, and that of Tf B was 0.002; in small numbers of other racial groups, Tf D gene frequencies ranged from 0.008 to 0.042. Serum albumin Medan and albumin Kuala Lumpur were found at low frequencies.
Supported by the University of California International Center for Medical Research (UC ICMR) through research grant AI 10051 to the Department of International Health, School of Medicine, University of California, San Francisco, from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, U.S. Public Health Service. 相似文献
Zusammenfassung Indonesier aus Nord-Sumatra sind auf Erythrocytenenzyme und Serum-Protein-Varianten untersucht worden. Die Genfrequenz von 6PGDC unter 264 Batak war 0,051; in kleinen Bevölkerungszahlen anderer rassischer Gruppen lag sie zwischen 0,0016 und 0,060. Die Genfrequenz von PGM 1 1 unter 272 Batak betrug 0.761; in kleinen Bevölkerungszahlen anderer rassischer Gruppen lag sie zwischen 0,700 und 0,808. PHI Typ 4-1 fand sich einmal unter 271 Batak, und PHI 4-1, PHI 3-1 und PHI 6-1 wurden je einmal unter 55 Chinesen festgestellt. Im MDH- und LDH-System fanden sich keine Varianten. Die Hp1-Genfrequenz bei 271 Batak war 0,284; in kleinen Bevölkerungszahlen anderer rassischer Gruppen lag sie zwischen 0,216 und 0,292. Die Häufigkeit des Tf D-Gens bei 262 Batak war 0,044 und die des Tf B-Gens 0,002; in kleinen Bevölkerungszahlen anderer rassischer Gruppen lag die Genfrequenz zwischen 0,008 und 0,042. Das Serumalbumin Medan und-albumin Kuala Lumpur hatten eine geringere Frequenz.
Supported by the University of California International Center for Medical Research (UC ICMR) through research grant AI 10051 to the Department of International Health, School of Medicine, University of California, San Francisco, from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, U.S. Public Health Service. 相似文献
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Muhammad Hussein Gasem Herman Kosasih Emiliana Tjitra Bachti Alisjahbana Muhammad Karyana Dewi Lokida Aaron Neal C. Jason Liang Abu Tholib Aman Mansyur Arif Pratiwi Sudarmono Suharto Tuti Parwati Merati Vivi Lisdawati Siswanto Sophia Siddiqui H. Clifford Lane INA-RESPOND 《PLoS neglected tropical diseases》2022,16(6)
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Hansen J. Kosasih Karena Last Fraser M. Rogerson Suzanne B. Golub Stephanie J. Gauci Vincenzo C. Russo Heather Stanton Richard Wilson Shireen R. Lamande Paul Holden Amanda J. Fosang 《The Journal of biological chemistry》2016,291(7):3197-3208
The metalloproteinase ADAMTS-5 (A
disintegrin and metalloproteinase with thrombospondin motifs) degrades aggrecan, a proteoglycan essential for cartilage structure and function. ADAMTS-5 is the major aggrecanase in mouse cartilage, and is also likely to be the major aggrecanase in humans. ADAMTS-5 is a multidomain enzyme, but the function of the C-terminal ancillary domains is poorly understood. We show that mutant ADAMTS-5 lacking the catalytic domain, but with a full suite of ancillary domains inhibits wild type ADAMTS activity, in vitro and in vivo, in a dominant-negative manner. The data suggest that mutant ADAMTS-5 binds to wild type ADAMTS-5; thus we tested the hypothesis that ADAMTS-5 associates to form oligomers. Co-elution, competition, and in situ PLA experiments using full-length and truncated recombinant ADAMTS-5 confirmed that ADAMTS-5 molecules interact, and showed that the catalytic and disintegrin-like domains support these intermolecular interactions. Cross-linking experiments revealed that recombinant ADAMTS-5 formed large, reduction-sensitive oligomers with a nominal molecular mass of ∼400 kDa. The oligomers were unimolecular and proteolytically active. ADAMTS-5 truncates comprising the disintegrin and/or catalytic domains were able to competitively block full-length ADAMTS-5-mediated aggrecan cleavage, measured by production of the G1-EGE373 neoepitope. These results show that ADAMTS-5 oligomerization is required for full aggrecanase activity, and they provide evidence that blocking oligomerization inhibits ADAMTS-5 activity. The data identify the surface provided by the catalytic and disintegrin-like domains of ADAMTS-5 as a legitimate target for the design of aggrecanase inhibitors. 相似文献
8.
Herman Kosasih Bachti Alisjahbana Susana Widjaja Nurhayati Quirijn de Mast Ida Parwati Patrick J. Blair Timothy H. Burgess Andre van der Ven Maya Williams 《PloS one》2013,8(11)
As dengue fever is undifferentiated from other febrile illnesses in the tropics and the clinical course is unpredictable, early diagnosis is important. Several commercial assays to detect dengue NS1 antigen have been developed; however, their performances vary and data is lacking from hyper-endemic areas where all four serotypes of dengue are equally represented. To assess the sensitivity of the Bio-Rad platelia Dengue NS1 antigen assay according to virus serotype, immune status, gender, and parameters of severe disease, acute sera from 220 individuals with confirmed dengue and 55 individuals with a non-dengue febrile illness were tested using the Bio-Rad platelia Dengue NS1 antigen assay. The overall sensitivity of the NS1 ELISA was 46.8% and the specificity was 100%. The sensitivity in primary infections was significantly higher than in secondary infections (100% vs. 35.7%). In secondary infections, the sensitivity of NS1 detection was highest in DENV-3 (47.1%), followed by DENV-1 (40.9%), DENV-2 (30%) and DENV-4 (27%) infections. NS1 was less frequently detected in sera with high titers of HI antibodies or in acute samples from patients whose pre-illness sera showed neutralizing antibodies to more than one serotype. The detection of NS1 was higher in females, severe cases, and individuals with lower platelet counts (<100,000/mm3). While the overall sensitivity of this NS1 ELISA is poor, our data suggest that in secondary infections, detection may be predictive of a more severe illness. 相似文献
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Rintis Noviyanti Farah Coutrier Retno A. S. Utami Hidayat Trimarsanto Yusrifar K. Tirta Leily Trianty Andreas Kusuma Inge Sutanto Ayleen Kosasih Rita Kusriastuti William A. Hawley Ferdinand Laihad Neil Lobo Jutta Marfurt Taane G. Clark Ric N. Price Sarah Auburn 《PLoS neglected tropical diseases》2015,9(5)