排序方式: 共有11条查询结果,搜索用时 15 毫秒
1.
Hiroshi Morita Yuichiro Tomizawa Jun Deguchi Tokio Ishikawa Hiroko Arai Kazumasa Zaima Takahiro Hosoya Yusuke Hirasawa Takayuki Matsumoto Katsuo Kamata Wiwied Ekasari Aty Widyawaruyanti Tutik Sri Wahyuni Noor Cholies Zaini Toshio Honda 《Bioorganic & medicinal chemistry》2009,17(24):8234-8240
Cassiarin A 1, a tricyclic alkaloid, isolated from the leaves of Cassia siamea (Leguminosae), shows powerful antimalarial activity against Plasmodium falciparum in vitro as well as P. berghei in vivo, which may be valuable leads for novel antimalarials. Interactions of parasitized red blood cells (pRBCs) with endothelium in aorta are especially important in the processes contribute to the pathogenesis of severe malaria. Nitric oxide (NO) reduces endothelial expression of receptors/adhesion molecules used by pRBC to adhere to vascular endothelium, and reduces cytoadherence of pRBC to vascular endothelium. Cassiarin A 1 showed vasorelaxation activity against rat aortic ring, which may be related with NO production. A series of a hydroxyl and a nitrogen-substituted derivatives and a dehydroxy derivative of 1 have been synthesized as having potent antimalarials against P. falciparum with vasodilator activity, which may reduce cytoadherence of pRBC to vascular endothelium. Cassiarin A 1 exhibited a potent antimalarial activity and a high selectivity index in vitro, suggesting that the presence of a hydroxyl and a nitrogen atom without any substituents may be important to show antimalarial activity. Relative to cassiarin A, a methoxy derivative showed more potent vasorelaxant activity, although it did not show improvement for inhibition of P. falciparum in vitro. These cassiarin derivatives may be promising candidates as antimalarials with different mode of actions. 相似文献
2.
M. Mabrouk Atalla H. Kheiralla Zeinab R. Hamed Eman A. Youssry Amani A. Abd El Aty Abeer 《Saudi Journal of Biological Sciences》2013,20(4):373-381
The properties of Trematosphaeria mangrovei laccase enzyme purified on Sephadex G-100 column were investigated. SDS–PAGE of the purified laccase enzyme showed a single band at 48 kDa. The pure laccase reached its maximal activity at temperature 65 °C, pH 4.0 with Km equal 1.4 mM and Vmax equal 184.84 U/mg protein. The substrate specificity of the purified laccase was greatly influenced by the nature and position of the substituted groups in the phenolic ring. The pure laccase was tested with some metal ions and inhibitors, FeSO4 completely inhibited laccase enzyme and also highly affected by (NaN3) at a concentration of 1 mM. Amino acid composition of the pure enzyme was also determined. Carbohydrate content of purified laccase enzyme was 23% of the enzyme sample. The UV absorption spectra of the purified laccase enzyme showed a single peak at 260–280 nm. 相似文献
3.
Matsuno Y Deguchi J Hirasawa Y Ohyama K Toyoda H Hirobe C Ekasari W Widyawaruyanti A Zaini NC Morita H 《Bioorganic & medicinal chemistry letters》2008,18(13):3774-3777
Two new cassane-type diterpenes, sucutiniranes A (1) and B (2), have been isolated from the seeds of Bowdichia nitida together with 6alpha-acetoxyvouacapane (3) and 6alpha,7beta-diacetoxyvouacapane (4), and the structures of 1 and 2 were elucidated by using 2D NMR data and chemical correlations. Sucutinirane A (1) and 3 showed a moderate cytotoxicity against human colon carcinoma COLO201 cells, and 6alpha,7beta-diacetoxyvouacapane (4) showed in vitro antiplasmodial activity against parasite Plasmodium falciparum 3D7. 相似文献
4.
Khalit Mohamad Yusuke Hirasawa Marc Litaudon Khalijah Awang A. Hamid A. Hadi Koichi Takeya Wiwied Ekasari Aty Widyawaruyanti Noor Cholies Zaini Hiroshi Morita 《Bioorganic & medicinal chemistry》2009,17(2):727-730
Three new limonoids, ceramicines B–D (1–3), have been isolated from the bark of Chisocheton ceramicus. Structures and stereochemistry of 1–3 were fully elucidated and characterized by 2D NMR analysis. Ceramicines exhibited a moderate antiplasmodial activity. 相似文献
5.
Ahmed M. Ismail Said M. Abdou Hassan Abdel Aty Adel H. Kamhawy Mohammed Elhinedy Mohammed Elwageh Atef Taha Amal Ezzat Hoda A. Salem Said Youssif Mohamed L. Salem 《Cytotechnology》2016,68(4):771-781
Patients with a decrease in limb perfusion with a potential threat to limb viability manifested by ischemic rest pain, ischemic ulcers, and/or gangrene are considered to have critical limb ischemia (CLI). Because of this generally poor outcome, there is a strong need for attempting any procedure to save the affected limb. The aim of this work is to evaluate the possibility to use stem cell therapy as a treatment option for patients with chronic critical lower limb ischemia with no distal run off. This study includes 20 patients with chronic critical lower limb ischemia with no distal run off who are unsuitable for vascular or endovascular option. These patients underwent stem cell therapy (SCT) by autologous transplantation of bone marrow derived mononuclear cells. 55 % of patients treated with SCT showed improvement of the rest pain after the first month, 60 % continued improvement of the rest pain after 6 months, 75 % after 1 year and 80 % after 2 years and continued without any deterioration till the third year. Limb salvage rate after STC was 80 % after the first year till the end of the second and third years. SCT can result in angiogenesis in patients with no-option CLI, providing a foundation for the application of this therapy to leg ischemia. 相似文献
6.
7.
Oshimi S Tomizawa Y Hirasawa Y Honda T Ekasari W Widyawaruyanti A Rudyanto M Indrayanto G Zaini NC Morita H 《Bioorganic & medicinal chemistry letters》2008,18(13):3761-3763
A new bischromone, chrobisiamone A (1) with an antiplasmodial activity has been isolated from the leaves of Cassia siamea and the structure was elucidated by 2D NMR analysis. Cyclization of 5-acetonyl-7-hydroxy-2-methylchromone (2) in the presence of ammonium acetate resulted in generation of cassiarin A (3) with an unprecedented tricyclic skeleton, supporting a biogenetic pathway proposed for 3. 相似文献
8.
In our search for new unconventional green-reducing agent, can be applied for biosynthesis of biologically active silver-nanoparticles, fruiting bodies (Ascocarps) of Truffle Terminia sp. were collected from the sandy desert of Hafr Al-Batin, Eastern Region, Saudi Arabia. The desert truffle showed the ability to reduce AgNO3 to Ag0 depending on their high content of proteins (1.74 mg/ml) in the aqueous extract of 30 mg/ml (w/v). The response surface methodology (RSM) with 13 experiments of 2–Factors–5–Levels central composite design was applied for controlling all possible combinations of AgNO3 concentrations and pH values of reaction mixture, which directly affect the particles morphology, size and biological activity. The antimicrobial effectiveness of all synthesized nanoparticles was evaluated against the pathogenic strains by agar diffusion method. The pathogenic Gram-positive Staphylococcus aureus, Bacillus subtilis, Lactobacillus cereus, and Gram-negative Escherichia coli, Salmonella enterica, yeast strain Candida albicans and the fungus Aspergillus niger were evaluated. The biologically active Truffle-AgNPs were characterized by UV–visible spectrophotometry, transmission electron microscopy (TEM), spectrum and dynamic light scatter (DLS), and Fourier Transformed Infrared (FTIR). Results obtained indicated that, the statistically controlled Truffle-AgNPs have great inhibitory role affecting different pathogenic strains, which gained much attention towards application of Hafr Al-Batin-Truffle as reducing and stabilizing biomaterial for green nano-drugs biosynthesis, to resist harmful pathogens threaten human health. 相似文献
9.
Nugroho AE Sugai M Hirasawa Y Hosoya T Awang K Hadi AH Ekasari W Widyawaruyanti A Morita H 《Bioorganic & medicinal chemistry letters》2011,21(11):3417-3419
Two new indole alkaloids, bisnicalaterine D (1), consisting of an eburnane and a corynanthe type of skeletons, and nicalaterine A (2) were isolated from the bark of Hunteria zeylanica. Their structures were elucidated by various spectroscopic data such as NMR and CD spectra. A series of bisnicalaterines and nicalaterine A showed potent antiplasmodial activity against Plasmodium falciparum 3D7. 相似文献
10.
Mohamed-Elamir F. Hegazy Ahmed A. El-Beih Ahmed R. Hamed Abeer A. Abd El Aty Naglaa S. Mohamed Paul W. Paré 《Bioorganic & medicinal chemistry letters》2017,27(16):3825-3828
While select eudesmane sesquiterpenes exhibit anti-neoplastic activity, tumor-inhibition for costic-acids has not been established. Here biological activity of 3-oxo-γ-costic acid (1), previously isolated from Chiliadenus montanus, as well as new sesquiterpenes (2–5) and the known derivative, 3-oxoeudesma-1,4,11(13)-trien-7-1061αH-l2-oic acid (6), all produced from 1 by the fungus Athelia rolfsii, are reported. Structures were elucidated using MS and NMR spectroscopy with activity-screening utilizing human colon- and lung-tumor lines, Caco-2 and A549 respectively. Compound 1 exhibited anti-proliferative activity against Caco-2 (IC50 39 µM) and 2 was active against A549 (IC50 74 µM) suggesting therapeutic potential for the original substrate and a bio-transformed product. 相似文献