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Zahra Shokati Eshkiki Mohammad Hossein Ghahremani Parisa Shabani Sattar Gorgani Firuzjaee Asie Sadeghi Hossein Ghanbarian Reza Meshkani 《Molecular and cellular biochemistry》2017,425(1-2):95-102
Protein tyrosine phosphatase 1B (PTP1B) has been shown to regulate multiple cellular events such as differentiation, cell growth, and proliferation; however, the role of PTP1B in differentiation of embryonic stem (ES) cells into cardiomyocytes remains unexplored. In the present study, we investigated the effects of PTP1B inhibition on differentiation of ES cells into cardiomyocytes. PTP1B mRNA and protein levels were increased during the differentiation of ES cells into cardiomyocytes. Accordingly, a stable ES cell line expressing PTP1B shRNA was established. In vitro, the number and size of spontaneously beating embryoid bodies were significantly decreased in PTP1B-knockdown cells, compared with the control cells. Decreased expression of cardiac-specific markers Nkx2-5, MHC-α, cTnT, and CX43, as assessed by real-time PCR analysis, was further confirmed by immunocytochemistry of the markers. The results also showed that PTP1B inhibition induced apoptosis in both differentiated and undifferentiated ES cells, as presented by increasing the level of cleaved caspase-3, cytochrome C, and cleaved PARP. Further analyses revealed that PTP1B inhibition did not change proliferation and pluripotency of undifferentiated ES cells. Taken together, the data presented here suggest that PTP1B is essential for proper differentiation of ES cells into cardiomyocytes. 相似文献
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Sadeghi Asie Bastin Ali Reza Ghahremani Hossein Doustimotlagh Amir Hossein 《Molecular biology reports》2020,47(5):3557-3566
Molecular Biology Reports - Rosmarinic acid (RA) is a potential herbal medicine and has received considerable attention due to its strong antioxidant properties. The aim of this study is to... 相似文献
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Kobra Bahrampour Juybari Komal Rizwan Sanaz Faramarz Asie Sadeghi Arian Amirkhosravi Mohammad Hadi Nematollahi Mehrnaz Mehrabani 《化学与生物多样性》2023,20(4):e202200721
During stem cell therapy, some issues, such as obscure fate of stem cells or their low survival rate in the body, should be addressed to boost their therapeutic efficiency. Nanotechnology offers a suitable solution to combat such limitations. Carbon quantum dots (CQDs) are carbon-based nanomaterials and may be used as multi-purpose compounds in stem cell therapy. CQDs are excellent choices for stem cell labeling thanks to their special features such as optical properties and good biocompatibility. Besides, they can modulate the biological function of stem cells, such as their proliferation, homing ability, and differentiation properties. Considering the charismatic feature of CQDs and their broad unique effect on stem cells, the current review aims to summarize the most advancements in this field. Hence, we first focused on CQDs synthesis and their applications. In the next section, the stem cell categories will be discussed, and the final part is dedicated to the recent research evaluating the impact of CQDs on stem cell therapy. 相似文献
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Resveratrol Ameliorates Palmitate‐Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and JNK/NF‐κB Pathway in a SIRT1‐Independent Mechanism
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