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1.
2.
Trigonopsis variabilis induced for D-amino acid oxidase and catalase was immobilized by entrapment in Polyacrylamide beads obtained by radiation
polymerisation. Permeabilization of the cells was found to be essential for optimal activity of the enzymes in free cells.
However, the process of entrapment itself was found to eliminate the permeability barrier of cells immobilized in Polyacrylamide.
The two enzymes exhibited a differential response on Polyacrylamide entrapment. Thus, D-amino acid oxidase activity was stabilized
to heat inactivation whereas catalase in the same cells showed a destabilization on entrapment in Polyacrylamide. The coimmobilized
enzyme preparation showed an operational half life of 7–9 days after which the D-amino acid oxidase activity remained stable
at a value 35–40% of that of the initial activity for a study period of 3 weeks. Coimmobilization of MnO2 was not effective in enhancing the operational life of the enzyme preparation. 相似文献
3.
Ashwini Deshpande K. Sankaran S. F. D'Souza G. B. Nadkarni 《Biotechnology Techniques》1987,1(1):55-58
Summary A relatively simple method has been described for the rapid purification of D-amino acid oxidase from Trigonopsis variabilis by hydrophobic chromatography on Phenyl-Sepharose CL-4B and negative adsorption on DEAE-cellulose. The purified enzyme had a specific activity of 22–24 units at 25°C and exhibited three bands on enzymatic staining. 相似文献
4.
The short-term and long-term effects of two most commonly used brown and black masheri were studied in Swiss mice and Syrian golden hamsters. In short-term studies, both the types of masheri extracts (ME) at 3/4 LD50 dose given ip did not have any effect on either liver or plasma vitamin C levels (both species). However, a decrease in liver vitamin A was observed only in hamsters injected with black ME. Similar effect was not observed in mice injected with both the types of masheri extracts. In long-term studies, when both the types of masheri were fed through diet at 10% level for 20 months, no effect was observed on hepatic or plasma vitamin C levels in mice (both sexes), while an increase in vitamin C levels was observed in black masheri diet fed hamsters. A depletion in liver vitamin A was observed in hamsters fed both the types of masheri. Such an effect was observed only in black masheri diet fed Swiss mice (both sexes) and brown masheri diet fed Swiss females. 相似文献
5.
The defatted starch was dispersed in NaOH (1 M) and neutralized with HCl (1 M). The amylose 1-butanol complex is adsorbed on defatted cellulose powder in the solvent system containing acetate buffer (pH 4.8,0.1 M) + urea (2 M) + 1-butanol (8.5%, v/v). The complex adsorbed on cellulose powder is separated by centrifugation (2418 g). The sediment is washed with the solvent system-I to obtain the intermediate fraction. The adsorbed amylose is eluted with urea (2 M) in acetate buffer (pH 4.8, 0.1 M). The amylose, intermediate fraction and amylopectin were precipitated with ethanol, washed free of urea and air dried. They were characterized by determining their blue value and beta -amylolysis limit. 相似文献
6.
Narasimha Pullaguri Ashwini Umale Anamika Bhargava 《Journal of biochemical and molecular toxicology》2023,37(2):e23244
Several scientific studies have suggested a link between increased exposure to pollutants and a rise in the number of neurodegenerative disorders of unknown origin. Notably, triclosan (an antimicrobial agent) is used in concentrations ranging from 0.3% to 1% in various consumer products. Recent studies have also highlighted triclosan as an emerging toxic pollutant due to its increasing global use. However, a definitive link is missing to associate the rising use of triclosan and the growing number of neurodegenerative disorders or neurotoxicity. In this article, we present systematic scientific evidence which are otherwise scattered to suggest that triclosan can indeed induce neurotoxic effects, especially in vertebrate organisms including humans. Mechanistically, triclosan affected important developmental and differentiation genes, structural genes, genes for signaling receptors and genes for neurotransmitter controlling enzymes. Triclosan-induced oxidative stress impacting cellular proteins and homeostasis which triggers apoptosis. Though the scientific evidence collated in this article unequivocally indicates that triclosan can cause neurotoxicity, further epidemiological studies may be needed to confirm the effects on humans. 相似文献
7.
Ashwini Kumar M. M. Husain Hasan Mukhtar C. R. Krishna Murti 《Journal of biosciences》1980,2(3):181-189
Glutathione-S-transferase (EC 2.5.1.18) activity was assayed in hepatic and extra-hepatic tissues of pigeons using l-chloro-2,4-dinitrobenzene
and 1,2-dichloro-4-nitrobenzene as substrates. Gluthathione-S-transferase activity towards 1-chloro-2,4-dinitrobenzene in
pigeon was in the order: kidney > liver > testes > brain > lung> heart. The enzyme activity with 1-chloro-2,4-dinitrobenzene
as substrate was 40–44 times higher in pigeon liver and kidney than that observed with 1,2-dichloro-4-dinitrobenzene as substrate.K
m values of hepatic and renal glutathione transferase with l-chloro-2,4-dinitrobenzene as substrate were 2.5 and 3 mM respectively.
Double reciprocal plots with varying reduced gluthathione concentrations resulted in biphasic curves with twoK
m values (liver 0.31 mM and 4mM; kidney 0.36 mM and 1.3 mM). The enzyme activity was inhibited by oxidized gluthathione in
a dose-dependent pattern. 3-Methylcholanthrene elicited about 50% induction of hepatic glutathione transferase activity whereas
phénobarbital was ineffective. 相似文献
8.
G V Bhide 《Steroids》1979,33(4):361-378
Asymmetric synthesis of D-homoestrone methyl ether was achieved by a new annalation reaction. 相似文献
9.
10.
Kshirsagar Parthraj R. Mohite Ashwini Suryawanshi Suresh Chavan Jaykumar J. Gaikwad Nikhil B. Bapat Vishwas A. 《Plant Cell, Tissue and Organ Culture》2021,144(2):383-396
Plant Cell, Tissue and Organ Culture (PCTOC) - The present study reports an optimized protocol for high frequency in vitro plant propagation through direct and indirect organogenesis, phytochemical... 相似文献