全文获取类型
收费全文 | 183篇 |
免费 | 23篇 |
出版年
2022年 | 2篇 |
2021年 | 7篇 |
2020年 | 5篇 |
2019年 | 6篇 |
2018年 | 8篇 |
2017年 | 4篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 6篇 |
2013年 | 12篇 |
2012年 | 19篇 |
2011年 | 15篇 |
2010年 | 11篇 |
2009年 | 10篇 |
2008年 | 8篇 |
2007年 | 4篇 |
2006年 | 4篇 |
2005年 | 8篇 |
2004年 | 8篇 |
2003年 | 7篇 |
2002年 | 6篇 |
2001年 | 3篇 |
2000年 | 8篇 |
1999年 | 9篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1993年 | 1篇 |
1992年 | 7篇 |
1991年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1975年 | 1篇 |
排序方式: 共有206条查询结果,搜索用时 15 毫秒
1.
E. Chevreau Y. Lespinasse M. Gallet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1985,71(2):268-277
Summary Electrophoresis of 7 pollen enzymes was applied to 5 progenies from controlled crosses and one self-progeny of apple. Segregation data were examined according to three kinds of hypotheses: monogenic disomic, bigenic disomic and tetrasomic inheritance Twenty codominant alleles and a recessive null were identified. Results provided evidence of bigenic disomic inheritance in most cases: 6 pairs of homoeologous loci carrying identical homoeoalleles were revealed; only 2 enzymes exhibited a simple monogenic control. Preferential pairing between pairs of homologous chromosomes in meiosis can be postulated. These results indicated an allopolyploid origin of apple genome. Fixed heterozygosity occurred for several enzymes, which is a typical feature of allopolyploidy. Loss of duplicate gene expression can account for the monogenic control of 2 of the enzymes.The results reported in this paper are part of a thesis by the first author for the degree of Docteur Ingénieur 相似文献
2.
Exposure to ethanol in man has been linked to an alteration of the immune surveillance system and reduced ability of the macrophage to undergo phagocytosis. Since ethanol has been suggested to alter membrane function and inhibit the production of calcium ionophore stimulated synthesis of prostaglandins and leukotrienes by the human neutrophil and transformed murine mast cell, the dose response effect of ethanol on the biosynthesis of icosanoids by the peritoneal macrophage during zymosan phagocytosis was studied. Peritoneal macrophages from two inbred strains of mice derived from a common stock (HS) and selected for sensitivity to ethanol (shoprt sleep [SS]/long sleep [LS]) were studies. Zymosan phagocytosis was found to lead to synthesis of LTC4 (70 ng/106 cells), 6-keto-PGF1a (5 ng/106 (3 ng/106 cells). For the HS macrophage, ethanol caused a dose dependent inhibition of these lipid mediators as well as inhibition of phagocytosis and release of beta-hexosaminidase. However, a difference was observed in arachidonate metabolism stimulated by phagocytosis between the LS and SS mice below 100 mM ethanol. The SS mouse had a 50% inhibition of cyclooxygenase products at 86 mM ethanol with no inhibition of lipoxygenase metabolites. The LS mice had a trend suggesting increased lipoxygenase metabolites below 100 mM ethanol. At these levels of ethanol which can be found in man, these results suggest there may be differential production of lipid mediators under genetic control. 相似文献
3.
A Dazord D Gallet J M Saez 《Biochemical and biophysical research communications》1977,76(4):1238-1246
Corticotropin (1–24) tetracosapeptide (ACTH1–24) induces a small but significant increase in the incorporation of radioactive leucine into trichloracetic insoluble proteins of a mouse adrenal cell line Y1. Neither cyclic AMP, nor cholera toxin or a nitrophenyl sulfenyl derivative of ACTH1–24 (NPS-ACTH1–24) have any effects.After being labelled with radioactive leucine in the presence or absence of ACTH, the cells were solbilized in 1 % sodium dodecylsulfate and subjected to 20 % sodium dodecylsulfate polyacrylamide gels electrophoresis. ACTH1–24 was found to induce a dramatic increase in the incorporation of radioactive leucine into a small peptide (MW 3500). This effect was mimicked by other steroidogenic compounds such as cholera toxin, cyclic AMP, NPS-ACTH1–24 but not by ACTH11–24, a non steroidogenic analogue of ACTH. 相似文献
4.
L Maurousset R Lemoine O Gallet S Delrot J L Bonnemain 《Biochimica et biophysica acta》1992,1105(2):230-236
Plasma membrane vesicles were prepared by phase partition from a microsomal fraction of broad bean (Vicia faba L.) leaf. In order to study the effects of sodium sulfite on active uptake of sucrose, the vesicles were artificially energized by a transmembrane pH gradient (delta pH) and/or a transmembrane electrical gradient (delta psi). At 1 mM, sulfite strongly inhibited sucrose uptake but did not affect the two components of the proton motive force, delta pH (measured by dimethyloxazolidine dione) and delta psi (measured by tetraphenylphosphonium). Moreover, sulfite did not inhibit the proton-pumping ATPase of the plasma membrane vesicles. These data demonstrate that sulfite may inhibit transport of photoassimilates in plant by a direct inhibition of the sucrose carrier of the plasma membrane. 相似文献
5.
ACTH in vivo stimulation of the synthesis of a specific mitochondrial protein in the rat 总被引:2,自引:0,他引:2
A Dazord D Gallet de Santerre J M Saez 《Biochemical and biophysical research communications》1981,98(4):885-891
Protein markers induced by hormones are the necessary probes to study hormone regulation of gene expression. We recently showed that ACTH was able to induce one of these markers in the cytosol of the rat adrenal (Dazord et al, Biochem. J. , 233–239, 1978). In this paper we described another protein marker whose localization is mitochondrial and whose MW is 134 K. Maximal stimulation is seen 2 hours after ACTH injection. Actinomycin D injected 30 min before or 1 hour after the hormone blocks the stimulation. In hypophysectomized rats both ACTH and cyclic AMP are able to stimulate the synthesis of this protein. 相似文献
6.
- The formation and reversion of spheroplasts of the diaminopimelic acid-auxotrophic mutant Escherichia coli K 12, 335, dap −, R+TEM in a medium lacking diaminopimelic acid have been investigated by microphotography: During their development from rod form cells to spheroplasts cells on slide-surface-agar preparations underwent two successive cell divisions in the course of which the cells retained their rod form. The cells formed by these divisions partitioned into a varying number of spheroplasts of different size. The reversion of spheroplasts to rod form cells, started by the addition of diaminopimelic acid showed two characteristic steps: Each spheroplast partitioned again into several spheroplast-like cell bodies; most of them reverted directly to rod form cells.
- The release of the R-factor mediated periplasmic TEM-β-lactamase, E. C. 3.4.2.6., into the growth medium during the development of spheroplasts attained more than 50% of the entire TEM-β-lactamase activity.
7.
8.
Fabien Coze Fran?oise Gilard Guillaume Tcherkez Marie-Jo?lle Virolle Armel Guyonvarch 《PloS one》2013,8(12)
Metabolic Flux Analysis is now viewed as essential to elucidate the metabolic pattern of cells and to design appropriate genetic engineering strategies to improve strain performance and production processes. Here, we investigated carbon flux distribution in two Streptomyces coelicolor A3 (2) strains: the wild type M145 and its derivative mutant M1146, in which gene clusters encoding the four main antibiotic biosynthetic pathways were deleted. Metabolic Flux Analysis and 13C-labeling allowed us to reconstruct a flux map under steady-state conditions for both strains. The mutant strain M1146 showed a higher growth rate, a higher flux through the pentose phosphate pathway and a higher flux through the anaplerotic phosphoenolpyruvate carboxylase. In that strain, glucose uptake and the flux through the Krebs cycle were lower than in M145. The enhanced flux through the pentose phosphate pathway in M1146 is thought to generate NADPH enough to face higher needs for biomass biosynthesis and other processes. In both strains, the production of NADPH was higher than NADPH needs, suggesting a key role for nicotinamide nucleotide transhydrogenase for redox homeostasis. ATP production is also likely to exceed metabolic ATP needs, indicating that ATP consumption for maintenance is substantial.Our results further suggest a possible competition between actinorhodin and triacylglycerol biosynthetic pathways for their common precursor, acetyl-CoA. These findings may be instrumental in developing new strategies exploiting S. coelicolor as a platform for the production of bio-based products of industrial interest. 相似文献
9.
Paul Faustin Seke Etet Lorella Vecchio Patrice Bogne Kamga Elias Nchiwan Nukenine Mauro Krampera Armel Hervé Nwabo Kamdje 《生物化学与生物物理学报:癌评论》2013
Wnts are a family of evolutionary-conserved secreted signaling molecules critically involved in a variety of developmental processes and in cell fate determination. A growing body of evidence suggests that Wnt signaling plays a crucial role in the influence of bone marrow stromal microenvironment on the balance between hematopoietic stem cell self-renewal and differentiation. Emerging clinical and experimental evidence also indicates Wnt signaling involvement in the disruption of the latter balance in hematologic malignancies, where the stromal microenvironment favors the homing of cancer cells to the bone marrow, as well as leukemia stem cell development and chemoresistance. In the present review, we summarize and discuss the role of the canonical Wnt signaling pathway in normal hematopoiesis and hematologic malignancies, with regard to recent findings on the stromal microenvironment involvement in these process and diseases. 相似文献