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DNA fragments of around 200 base pair (average size) have been covalently crosslinked with 8-methoxypsoralen under 365 nm UV light. The photoadduct, induced antibodies in rabbits with a titer of > 1:12,800 by direct bindng ELISA. Binding data showed that the induced antibodies are conformation-specific recognizing restricted conformational change at site of crosslinking. Human autoantibodies against DNA, bound not only to native DNA but to the photomodified DNA fragment as well. In addition, binding patterns of SLE sera obtained from different patients were remarkably similar, indicating the recognition of altered conformation of the modified polymer by naturally occurring SLE anti-DNA autoantibodies. 相似文献
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Mohsen A. F. El-Hazmi Hassan M. Bahakim Arjumand S. Warsy Abdulkarim Al-Momen Abdullah Al-Wazzan Ibrahim Al-Fawwaz Sameer Huraib Mohammad Harakati 《Molecular and cellular biochemistry》1993,124(1):17-22
Sickle cell anaemia (SCA) exhibits significant variations in clinical presentation in different populations for which several genetic factors including SCA-associated -and -thalassaemias, G-6-PD deficiency and elevated Hb F level have been implicated as possible ameliorating factors. Saudi Arabia is unique in that mild and severe forms of the disease occur at a high frequency. We investigated the G/A ratio and Hb F level and correlated these values with the severity of SCA. The results showed that Hb F level varies significantly in both groups of patients with no evident correlation with the mild clinical manifestations. However, G/A ratio correlated significantly with the disease severity where a high ratio was observed in patients with the mild and a low ratio in patients with the severe disease. The results are evaluated and discussed in the light of correlation studies and regression analysis. 相似文献
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Tsipouri V Curtin JA Nolan PM Vizor L Parsons CA Clapham CM Latham ID Rooke LJ Martin JE Peters J Hunter AJ Rogers D Rastan S Brown SD Fisher EM Spurr NK Gray IC 《Comparative and Functional Genomics》2004,5(2):123-127
Three mutant mice with pigmentation phenotypes were recovered from a genomewide random mouse chemical mutagenesis study. White toes (Whto; MGI:1861986), Belly spot and white toes (Bswt; MGI:2152776) and Dark footpads 2 (Dfp2; MGI:1861991) were identified following visual inspection of progeny from a male exposed to the point mutagen ethylnitrosourea (ENU). In order to rapidly localize the causative mutations, genome-wide linkage scans were performed on pooled DNA samples from backcross animals for each mutant line. Whto was mapped to proximal mouse chromosome (Mmu) 7 between Cen (the centromere) and D7Mit112 (8.0 cM from the centromere), Bswt was mapped to centric Mmul between D1Mit214 (32.1 cM) and D1Mit480 (32.8 cM) and Dfp2 was mapped to proximalMmu4 between Cen and D4Mit18 (5.2 cM). Whto, Bswt and Dfp2 may provide novel starting points in furthering the elucidation of genetic and biochemical pathways relevant to pigmentation and associated biological processes. 相似文献
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Casañola-Martín GM Marrero-Ponce Y Khan MT Ather A Sultan S Torrens F Rotondo R 《Bioorganic & medicinal chemistry》2007,15(3):1483-1503
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Effect of Pre‐ and Post–Combined Multidoses of Epigallocatechin Gallate and Coenzyme Q10 on Cisplatin‐Induced Oxidative Stress in Rat Kidney
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Sabiha Fatima Noura Al‐Mohaimeed Sadia Arjumand Naheed Banu Noura Al‐Jameil Yazeed Al‐Shaikh 《Journal of biochemical and molecular toxicology》2015,29(2):91-97
The nephroprotective effect of coenzyme Q10 and epigallocatechin gallate was investigated in rats with acute renal injury induced by a single nephrotoxic dose of cisplatin. Two days prior to cisplatin administration, epigallocatechin gallate and coenzyme Q10 alone and in four different combinations were given for 6 days. The treatment with antioxidants significantly protected the cisplatin‐induced increase in the levels of blood urea nitrogen and serum creatinine. Both the antioxidants alone or in different combinations significantly compensated the increased malondialdehyde and reduced glutathione levels. Moreover, the decrease in the activities of superoxide dismutase, catalase, and glutathione peroxidase and the concentration of selenium, zinc, and copper ions were significantly attenuated in renal tissue. In conclusion, epigallocatechin gallate and coenzyme Q10 are equally effective against cisplatin‐induced nephrotoxicity, whereas the intervention by combining these two antioxidants was found to be highly effective at low doses in attenuating oxidative stress in rat kidney. 相似文献
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Tertius A. Hough Patrick M. Nolan Vicky Tsipouri Ayo A. Toye Ian C. Gray Michelle Goldsworthy Lee Moir Roger D. Cox Sian Clements Peter H. Glenister John Wood Rachael L. Selley Mark A. Strivens Lucie Vizor Stefan L. McCormack Josephine Peters Elizabeth M. Fisher Nigel Spurr Sohaila Rastan Joanne E. Martin Steve D.M. Brown A. Jacqueline Hunter 《Mammalian genome》2002,13(10):595-602
We used ENU mutagenesis in the mouse for the rapid generation of novel mutant phenotypes for both gene function studies and use as new animal models of human disease (Nolan et al. 2000b). One focus of the program was the development of a blood biochemistry screen. At 8-12 weeks of age, approximately 300 ml of blood was collected from F1 offspring of ENU mutagenized male mice. This yielded approximately 125 ml of plasma, used to perform a profile of 17 standard biochemical tests on an Olympus analyzer. Cohorts of F1 mice were also aged and then retested to detect late onset phenotypes. In total, 1,961 F1s were screened. Outliers were identified by running means and standard deviations. Of 70 mice showing consistent abnormalities in plasma biochemistry, 29 were entered into inheritance testing. Of these, 9 phenotypes were confirmed as inherited, 10 found not to be inherited, and 10 are still being tested. Inherited mutant phenotypes include abnormal lipid profiles (low total and HDL cholesterol, high triglycerides); abnormalities in bone and liver metabolism (low ALP, high ALP, high ALT, and AST); abnormal plasma electrolyte levels (high sodium and chloride); as well as phenotypes of interest for the study of diabetes (high glucose). The gene loci bearing the mutations are currently being mapped and further characterized. Our results have validated our biochemical screen, which is applicable to other mutagenesis projects, and we have produced a new set of mutants with defined metabolic phenotypes. 相似文献
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The risk posed by the quantity of heavy metal lead present in Ca supplements is of grave concern. Some lead levels have been
measured up to the extent of regulatory limit set by the United States. Calcium supplements inevitably get contaminated with
lead as both are naturally occurring elements having the same charge density. Therefore, it is imperative to indicate the
level of this toxic metal in these supplements in order to create awareness among consumers. The calcium in the supplements
is derived from natural as well as synthetic/refined sources (chelated or non-chelated). In this study, a sophisticated analytical
technique, atomic absorption spectrometer (both with FAAS and GFAAS modes of atomization), was used for the purpose of analyzing
Pb contents in 27 commonly used Ca supplements manufactured by different national and multinational companies. The daily intake
of lead through these supplements was calculated. Only 10% of the calcium supplements analyzed met the criteria of acceptable
Pb levels (1.5 μg/daily dose) in supplements/consumer products set by the United States. It was also found that Pb intake
was highest in chelated calcium supplements whereas lowest through calcium supplements with vitamin D formulation. The Pb
concentration in calcium supplements was significantly increased (p < 0.001) according to their composition. In order to validate our results from the study conducted, IAEA-certified reference
material (animal bone, H-5) was analyzed for Pb levels. The limit of detection of the method used was 0.05 μg/g and a 95%
lead recovery of IAEA-certified reference material (animal bone, H-5). 相似文献
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Arjumand Sohaila Shiyam Sunder Tikmani Iqtidar Ahmed Khan Huba Atiq Ali Syed Muhammad Akhtar Prem Kumar Kishwer Kumar 《PloS one》2014,9(7)