全文获取类型
收费全文 | 128篇 |
免费 | 1篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 2篇 |
2020年 | 1篇 |
2019年 | 4篇 |
2018年 | 5篇 |
2017年 | 4篇 |
2016年 | 5篇 |
2014年 | 4篇 |
2013年 | 3篇 |
2012年 | 9篇 |
2011年 | 11篇 |
2010年 | 7篇 |
2009年 | 11篇 |
2008年 | 5篇 |
2007年 | 5篇 |
2006年 | 5篇 |
2005年 | 7篇 |
2004年 | 6篇 |
2003年 | 4篇 |
2002年 | 3篇 |
2001年 | 1篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1995年 | 2篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1987年 | 3篇 |
1985年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1972年 | 1篇 |
1967年 | 1篇 |
1965年 | 1篇 |
1916年 | 1篇 |
排序方式: 共有129条查询结果,搜索用时 15 毫秒
1.
The effect of cooling rate, freeze-drying suspending fluid and culture age on the preservation of Campylobacter pylori 总被引:1,自引:1,他引:0
The effects of freezing rate, suspending fluid and age of culture on the ability of four strains of Campylobacter pylori to survive and recover from freeze-drying were examined. Freeze-drying by standard procedures generally resulted in an overall loss in viability of between 3 and 7 log units. The exact cause of poor recovery by C. pylori was not established but strain differences were detected, with NCTC 11637 (type strain) surviving better than NCTC 11638 and NCTC 11639. Recovery of the poorest growing strain (NE 26695) was notably more erratic. The largest loss in viability occurred at the primary drying stage. Losses resulting from freezing and secondary drying were less marked and the rate of freezing had only a marginal effect on recovery. Nineteen different freeze-drying suspending fluids were investigated. Overall the best recovery results were obtained with 5% inositol-broth (or horse serum) plus 25% glucose, at pH 7.0, in which loss of viability was typically about 4 log units. Other factors, such as age of culture and number of viable bacteria in the before-dry suspension, did not have a significant effect on survival. We conclude from these results that C. pylori can survive freeze-drying, albeit in small numbers, but the degree of recovery is apparently largely strain dependent. 相似文献
2.
T. A. Gudasheva K. N. Koliasnikova T. A. Antipova S. B. Seredenin 《Doklady. Biochemistry and biophysics》2016,469(1):273-276
It was shown for the first time that the endogenous cyclic dipeptide cycloprolylglycine (CPG) at concentrations of 10–7 and 10–3 M and piracetam at a concentration of 10–3 M increased the content of brainderived neurotrophic factor (BDNF) in the culture of neuronal cells in normal state and under conditions of glutamate and 6-oxydopamine neurotoxicity. This may indicate the possible involvement of BDNF in the mechanism of action of neuropeptide CPG and piracetam. 相似文献
3.
本文记述采自新疆的皿蛛科蜘蛛一新届——颚齿蛛属Maxillodens gen.nov.及其一新种——鞭状颚齿蛛M.flageuatus sp.nov。 相似文献
4.
Rystsov G. K. Antipova T. V. Zaitsev K. V. Zemskova M. Yu. 《Russian Journal of Bioorganic Chemistry》2021,47(1):307-316
Russian Journal of Bioorganic Chemistry - It has been found that monasnicotinic acid (MNA) isolated from the fungus Aspergillus cavernicola VKM F-906 reduces the proliferation and migration of... 相似文献
5.
A. G. Kozlovskii V. P. Zhelifonova T. V. Antipova 《Applied Biochemistry and Microbiology》2013,49(1):1-10
Fungi of the genus Penicillium isolated from little studied habitats are able to synthesize both previously known and new physiologically active compounds with diverse structures. They include secondary metabolites of alkaloid nature, i.e., ergot alkaloids, diketopiperazines, quinolines, quinazolines, benzodiazepines, and polyketides. We discuss the use of profiles of secondary metabolites for taxonomy purposes. Studying the physicochemical characteristics of producers of biologically active compounds showed that the biosynthesis of alkaloids is initiated on the first days of cultivation and proceeds simultaneously with growth. The cyclic character of alkaloid accumulation was recorded related to the processes of alkaloid biosynthesis, excretion from cells, degradation in culture fluid, and consumption by cells. Synchronic variations in the concentrations of intracellular tryptophan and alkaloids are necessary for the regulation of the optimal quantity of tryptophan necessary for the culture. 相似文献
6.
Sazhina N. N. Antipova A. S. Semenova M. G. Palmina N. P. 《Russian Journal of Bioorganic Chemistry》2019,45(1):34-41
Russian Journal of Bioorganic Chemistry - Design and research of liposome structures on the basis of soy phosphatidylcholine (PC) as nanocontainers for delivery of various functional nutraceuticals... 相似文献
7.
Slizhikova DK Zinov'eva MV Kuz'min DV Snezhkov EB Shakhparonov MI Dmitriev RI Antipova NV Zavalova LL Sverdlov ED 《Molekuliarnaia biologiia》2007,41(4):659-665
Secretory polymeric immunoglobulins (IgA dimers and IgM pentamers) are unique in that, apart from L- and H-chains, they contain J-chains responsible for their oligomerization. These antibodies are part of the local adaptive immune system acting on mucosa membranes of the respiratory and digestive systems as the first protection barrier to potential infectious agents. Secretory polymeric immunoglobulins are produced by highly specific B-cells and actively transported to the surface of mucosa membrane through epithelium cells. Therefore, their synthesis and J-chain content are dependent upon epithelium translocation function and condition that are markedly affected by tumorous transformation. Here, we used RT-PCR and immunoblotting to study of the J-chain content and its mRNA expression level in normal and tumorous tissues in lung squamous cell cancer and adenocarcinoma at various stages of disease progression. 相似文献
8.
Kirichuk VF Ivanov AN Antipova ON Krenitskiĭ AP Maĭborodin AV Tupikin VD Betskiĭ OV 《Tsitologiia》2007,49(6):484-490
The influence of electromagnetic waves of terahertz range at the frequencies of molecular spectrum o nitric oxide radiation and absorption on functional activity of thrombocytes in white rats under long-term stress has been studied. It has been shown that courses of THzF treatment applied during the stress can prevent and restore disorders in thrombocytes aggregative function. The stress factor does not induce characteristic of stress-reaction disturbances of microcirculation in animals treated with the preventive course of THzF. 相似文献
9.
10.
Kozlovskiĭ AG Zhelifonova VP Antipova TV 《Prikladnaia biokhimiia i mikrobiologiia》2005,41(5):568-572
Quinocitrinines and ergot alkaloids are synthesized by the strain Penicillium citrinum VKM FW-800 as the culture grows. The major part of these secondary metabolites are secreted into the medium. In the phase of growth deceleration, these metabolites were partly absorbed by the producer cells. Zinc ions stimulated both the primary and secondary metabolic processes. Addition of this microelement into the culture medium stimulated biomass accumulation and the synthesis of clavine alkaloids and quinocitrinines. 相似文献