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Bubb Annesca Ehlers Karen Kotze Antoinette Grobler J. Paul 《European Journal of Wildlife Research》2011,57(4):971-975
Sampling methods that allow DNA collection without the physical handling of animals are popular in conservation genetics,
but DNA isolated from faecal samples may be degraded, potentially leading to erroneous microsatellite genotyping results.
We collected baboon faecal samples from fresh to 1-week post-defecation in a controlled sampling environment and preserved
these using three storage techniques. After DNA isolation and quantification, the samples were genotyped at eight microsatellite
loci. Quantitatively, DNA yield was highest when using silica as storage medium. However, microsatellite amplification from
samples stored in 95% ETOH were most successful, with 100% success for fresh samples and dropping only marginally to 87.5%
of loci for samples collected after 1 week. This disparity between quantitative and qualitative results suggests that total
DNA concentrations do not necessarily provide a reliable indication of the amount of target DNA present in a DNA isolate.
Our results nevertheless confirm that microsatellite fragments can successfully be amplified from faecal samples up to 1 week
post-defecation, with careful selection of storage protocols and loci. 相似文献
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