全文获取类型
收费全文 | 23529篇 |
免费 | 1709篇 |
国内免费 | 9篇 |
出版年
2023年 | 117篇 |
2022年 | 213篇 |
2021年 | 597篇 |
2020年 | 363篇 |
2019年 | 480篇 |
2018年 | 608篇 |
2017年 | 498篇 |
2016年 | 840篇 |
2015年 | 1269篇 |
2014年 | 1394篇 |
2013年 | 1726篇 |
2012年 | 2053篇 |
2011年 | 1987篇 |
2010年 | 1243篇 |
2009年 | 1062篇 |
2008年 | 1449篇 |
2007年 | 1354篇 |
2006年 | 1241篇 |
2005年 | 1139篇 |
2004年 | 1038篇 |
2003年 | 996篇 |
2002年 | 807篇 |
2001年 | 169篇 |
2000年 | 158篇 |
1999年 | 177篇 |
1998年 | 150篇 |
1997年 | 137篇 |
1996年 | 116篇 |
1995年 | 102篇 |
1994年 | 108篇 |
1993年 | 106篇 |
1992年 | 96篇 |
1991年 | 99篇 |
1990年 | 105篇 |
1989年 | 85篇 |
1988年 | 88篇 |
1987年 | 78篇 |
1986年 | 75篇 |
1985年 | 84篇 |
1984年 | 65篇 |
1983年 | 68篇 |
1982年 | 46篇 |
1981年 | 55篇 |
1980年 | 44篇 |
1979年 | 57篇 |
1978年 | 49篇 |
1977年 | 39篇 |
1976年 | 37篇 |
1975年 | 44篇 |
1973年 | 33篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
1.
Sebastián Moran Miguel Vizoso Anna Martinez-Cardús Antonio Gomez Xavier Matías-Guiu Sebastián M Chiavenna Andrés G Fernandez Manel Esteller 《Epigenetics》2014,9(6):829-833
A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array. 相似文献
2.
3.
The distribution of sodium-potassium-activated adenosinetriphosphatase (Na+-K+-ATPase) in the alveolar portion of rat lungs was examined by indirect immunofluorescence with the use of a mouse monoclonal anti-rat Na+-K+-ATPase and by ultrastructural cytochemistry using p-nitrophenylphosphate as substrate. The reaction was inhibitable by 10 mM ouabain or by the omission of K+ from the reaction mixture. Cysteine or levamisole was used to inhibit alkaline phosphatase activity. By immunofluorescence, staining was confined to cuboidal cells in alveolar spaces. These were tentatively identified as type II pneumocytes. By ultrastructural cytochemistry reaction product was present on the cytoplasmic side of the basolateral membranes of type II pneumocytes. No reaction product was observed in type I pneumocytes or in endothelium. These results indicate that type II pneumocytes contain more Na+-K+-ATPase, an enzyme important in vectorial electrolyte transport, than type I pneumocytes or endothelial cells. More sensitive methods, however, are required to determine the amounts and distribution of this enzyme in type I pneumocytes and pulmonary vascular endothelial cells. 相似文献
4.
5.
6.
Anna Morcom 《The journal of the Royal Anthropological Institute》2014,20(4):788-789
7.
8.
9.
10.
Kazazoglou Theodosia Panagiotou Christina Mihailidou Chrysovalantou Kokkinopoulou Ioanna Papadopoulou Anna Moutsatsou Paraskevi 《Molecular and cellular biochemistry》2021,476(12):4471-4485
Molecular and Cellular Biochemistry - Glucocorticoids (GCs) regulate astrocyte function, while glutamine synthetase (GS), an enzyme highly expressed in astrocytes, is one of the most remarkable... 相似文献