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排序方式: 共有116条查询结果,搜索用时 15 毫秒
1.
Structure and novel exons of the human tau gene. 总被引:11,自引:0,他引:11
2.
The effect of delayed female mating for the mushroom fungus gnat Lycoriella ingenua is investigated. We examine the effect of delaying female mating on the fertility and egg viability of female flies that have a mating delay of 0–5 days after emergence. Male fly age is held constant. Female age does not impact male acceptance and most flies copulate within seconds of pairing. We find that female flies experiencing mating delays of 0–4 days after emergence lay a similar number of eggs onto artificial substrates. Females that experience a mating delay of 5 days lay 54% fewer eggs than those that mate on day 0 (day of emergence). There is no effect of mating delay on the percentage of larvae that emerge. The results of the present study indicate that mating delays have little effect on the fertility or fecundity of the mushroom fungus pest L. ingenua. 相似文献
3.
Myosin Va binding to neurofilaments is essential for correct myosin Va distribution and transport and neurofilament density 下载免费PDF全文
Rao MV Engle LJ Mohan PS Yuan A Qiu D Cataldo A Hassinger L Jacobsen S Lee VM Andreadis A Julien JP Bridgman PC Nixon RA 《The Journal of cell biology》2002,159(2):279-290
The identification of molecular motors that modulate the neuronal cytoskeleton has been elusive. Here, we show that a molecular motor protein, myosin Va, is present in high proportions in the cytoskeleton of mouse CNS and peripheral nerves. Immunoelectron microscopy, coimmunoprecipitation, and blot overlay analyses demonstrate that myosin Va in axons associates with neurofilaments, and that the NF-L subunit is its major ligand. A physiological association is indicated by observations that the level of myosin Va is reduced in axons of NF-L-null mice lacking neurofilaments and increased in mice overexpressing NF-L, but unchanged in NF-H-null mice. In vivo pulse-labeled myosin Va advances along axons at slow transport rates overlapping with those of neurofilament proteins and actin, both of which coimmunoprecipitate with myosin Va. Eliminating neurofilaments from mice selectively accelerates myosin Va translocation and redistributes myosin Va to the actin-rich subaxolemma and membranous organelles. Finally, peripheral axons of dilute-lethal mice, lacking functional myosin Va, display selectively increased neurofilament number and levels of neurofilament proteins without altering axon caliber. These results identify myosin Va as a neurofilament-associated protein, and show that this association is essential to establish the normal distribution, axonal transport, and content of myosin Va, and the proper numbers of neurofilaments in axons. 相似文献
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Theodore G. Andreadis 《Journal of invertebrate pathology》1985,46(1):31-46
Amblyospora infections in Aedes stimulans are transovarially transmitted by females infected in the previous year. Pathogen development in progeny is dimorphic and host sex dependent. In males, the pathogen invades fat body tissue and undergoes an extensive developmental sequence which kills the host and results in the formation of eight haploid spores enclosed in an accessory membrane that are not infectious to other larvae. In females, the pathogen invades host oenocytes and undergoes a simple developmental sequence which has no detrimental affect on longevity, fecundity, oviposition, or egg hatch, and results in the formation of binucleated spores that infect the ovaries and ensure transmission to the next generation. Transovarial transmission is continuous and is the major way in which these microsporidia are maintained from year to year, but is incapable of maintaining infections in breeding populations because of low transmission rates and is not sufficient to account for the types and levels of infection observed in the field. Horizontal transmission is reported for the first time. It occurs sporadically during the early stages of larval subsequently disseminated to oenocytes of adult hosts and are transovarially transmitted by females to filial host generations. This pathway of transmission provides the necessary mechanism whereby these microsporidia can reenter the mosquito population and thus perpetuate themselves. 相似文献
6.
Transgenic mouse model of tauopathies with glial pathology and nervous system degeneration 总被引:13,自引:0,他引:13
Frontotemporal dementias (FTDs), including corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP), are neurodegenerative tauopathies characterized by widespread CNS neuronal and glial tau pathologies, but there are no tau transgenic (Tg) mice that model neurodegeneration with glia tau lesions. Thus, we generated Tg mice overexpressing human tau in neurons and glia. No neuronal tau aggregates were detected, but old mice developed Thioflavin S- and Gallyas-positive glial tau pathology resembling CBD astrocytic plaques. Tau-immunoreactive and Gallyas-positive oligodendroglial coiled bodies (similar to CBD and PSP), glial degeneration, and motor deficits were associated with age-dependent accumulations of insoluble hyperphosphorylated human tau and tau immunopositive filaments in degenerating glial cells. Thus, tau-positive glial lesions similar to human FTDs occur in these Tg mice, and these pathologies are linked to glial and axonal degeneration. 相似文献
7.
The expression of sarcomeric muscle-specific contractile protein genes in BC3H1 cells: BC3H1 cells resemble skeletal myoblasts that are defective for commitment to terminal differentiation 总被引:7,自引:4,他引:7 下载免费PDF全文
M B Taubman C W Smith S Izumo J W Grant T Endo A Andreadis B Nadal-Ginard 《The Journal of cell biology》1989,108(5):1799-1806
The BC3H1 cell line has been used widely as a model for studying regulation of muscle-related proteins, such as the acetylcholine receptor, myokinase, creatine kinase, and actin. These cells, derived from a nitrosourea-induced mouse brain neoplasm, have some of the morphological characteristics of smooth muscle and have been shown to express the vascular smooth muscle isoform of alpha-actin. To provide further information about the contractile protein phenotype of BC3H1 and to gain additional insights into the possible tissue of origin of these cells, we have examined the expression of a battery of contractile protein genes. During rapid growth, subconfluent BC3H1 cells express the nonmuscle isoform of alpha-tropomyosin (alpha-Tm) and the nonsarcomeric isoforms of myosin heavy and light chains (MHCs and MLCs, respectively), but do not express troponin T(TnT). However, when BC3H1 cells differentiate in response to incubation in serum-deprived medium or upon approaching confluence, they express TnT as well as sarcomeric muscle isoforms of MHC, MLC 2 and 3, alpha-Tm, and alpha-actin. These results suggest that BC3H1 is a skeletal muscle cell line of ectodermal origin that is defective for commitment to terminal differentiation. 相似文献
8.
Andreadis SS Alvarez-Alfageme F Sánchez-Ramos I Stodola TJ Andow DA Milonas PG Savopoulou-Soultani M Castánera P 《Journal of economic entomology》2007,100(1):195-201
The high-dose/refuge strategy is considered as the main strategy for delaying resistance in target pests to genetically modified crops that produce insecticidal proteins derived from Bacillus thuringiensis Berliner. This strategy is based on a key assumption that resistance alleles are initially rare (<10(-3)). To test this assumption, we used an F2 screen on natural populations of Sesamia nonagrioides Lefebvre (Lepidoptera: Noctuidae) from Greece and Spain. In total, 75 lines from Greece and 85 lines from Spain were screened for survival of F2 larvae on Cry1Ab corn, Zea mays L., leaves. No major resistance alleles were found. The frequency of resistance alleles in the Greek population was <9.7 x 10(-3) with 95% probability, which was very similar to that of the Spanish population (<8.6 x 10(-3) with 95% probability), and the expected frequencies were 3.2 x 10(-3) (0-0.0097) and 2.9 x 10(-3) (0-0.0086) in Greece and Spain (pooled 1.5 x 10(-3)). The experiment-wise detection probability of resistance was 94.0 and 97.5% for the Greek and the Spanish population, respectively. Evidence of alleles conferring partial resistance to Cry1Ab was found only for the Greek population. The frequency of alleles for partial resistance was estimated as 6.5 x 10(-3) with a 95% credibility interval between 8 x 10(-4) and 17.8 x 10(-3) and a detection probability of 94%. Our results suggest that the frequency of alleles conferring resistance to CrylAb, regarding the population of S. nonagrioides, may be rare enough so that the high-dose/refuge strategy could be applied with success for resistance management. 相似文献
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Stefan TG Bruijnen Mignon AC van der Weijden Joannes P Klein Otto S Hoekstra Ronald Boellaard J Christiaan van Denderen Ben AC Dijkmans Alexandre E Voskuyl Irene E van der Horst-Bruinsma Conny J van der Laken 《Arthritis research & therapy》2012,14(2):R71