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Olga N. Kulaeva Anastasiya B. Fedina Emiliya A. Burkhanova Natalya N. Karavaiko Marat Ya. Karpeisky Igor B. Kaplan Michael E. Taliansky Joseph G. Atabekov 《Plant molecular biology》1992,20(3):383-393
Exogenous human interferon 2 (IFN) and 2–5 oligoadenylates (2–5A) have been shown to cause at least a dual physiological effect in tobacco and wheat: (i) increased cytokinin activity and (ii) induced synthesis of numerous proteins, among which members of two groups of stress proteins have been identified, namely pathogenesis-related (PR) and heat shock (HS) proteins. These effects were observed only by low concentrations of these substances: IFN at 0.1–1 u/ml and 2–5A at 1–10 nM. 相似文献
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Dmitriev Alexey A. Kudryavtseva Anna V. Krasnov George S. Koroban Nadezhda V. Speranskaya Anna S. Krinitsina Anastasia A. Belenikin Maxim S. Snezhkina Anastasiya V. Sadritdinova Asiya F. Kishlyan Natalya V. Rozhmina Tatiana A. Yurkevich Olga Yu. Muravenko Olga V. Bolsheva Nadezhda L. Melnikova Nataliya V. 《BMC plant biology》2016,16(3):139-146
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Alexey A. Dmitriev Anna V. Kudryavtseva George S. Krasnov Nadezhda V. Koroban Anna S. Speranskaya Anastasia A. Krinitsina Maxim S. Belenikin Anastasiya V. Snezhkina Asiya F. Sadritdinova Natalya V. Kishlyan Tatiana A. Rozhmina Olga Yu. Yurkevich Olga V. Muravenko Nadezhda L. Bolsheva Nataliya V. Melnikova 《BMC plant biology》2016,16(3):237
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Simonenko E. Yu. Pryadun V. V. Ivanova A. A. Burmistrova E. V. Vasiliev A. N. Yakovenko S. A. 《Biophysics》2019,64(1):1-6
Biophysics - Abstract—The efficiency of cryoprotectants used to protect cells from damage is usually evaluated by the changes in vital cell parameters after a freezing–thawing cycle.... 相似文献
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Lisovskaya IL Shurkhina ES Yakovenko EE Tsvetaeva NV Kolodei SV Shcherbinina SP Ataullakhanov FI 《Biorheology》1999,36(4):299-309
We have previously proposed the osmofiltration method based on a modified Hanss hemorheometer to analyze distributions of erythrocytes in their ability to pass through membrane filters with 3 microns pores. Upon decrease in medium osmolality (u) the erythrocyte volume increases. When cell volume becomes V = Vcr at u = ucr, such cell loses its ability to pass through a 3 microns pore. The flow rate of erythrocyte suspension containing cells with different ucr through a filter gradually decreases with decreasing medium osmolality. This rate becomes zero at some u = omega, when the number of non-filterable cells in the applied sample approaches the number of pores in filter. Experimental determination of the dependencies of the filtration rate on medium osmolality for various hematocrit values allows to obtain omega for each hematocrit and, thereby, to assess the distribution of erythrocytes in ucr. Here, we propose a simplified version of this method, which allows screening of the erythrocytes in heterogeneous suspensions for the distribution in ucr by measuring omega for only two hematocrit values, 0.1% and 1%. Applications of the proposed method are exemplified by analysing the erythrocyte populations of healthy donors, of patients with microspherocytosis, hemochromatosis and normal erythrocyte populations in an acidic environment. 相似文献
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Yakovenko O Blyakhman F Pollack GH 《American journal of physiology. Cell physiology》2002,283(3):C735-C742
In attempting to deduce the size of theelementary molecular translation step, recent experiments using singlemyosin molecules translating over actin filaments have shown aconsistent step size of 5.4 nm (10, 21). We have carriedout parallel measurements on single myofibrils from rabbit cardiacmuscle and bumblebee flight muscle. Activated specimens were releasedor stretched with a motor-imposed ramp, and the time course of lengthof individual sarcomeres was measured by projecting the image of thestriations onto a linear photodiode array and tracking the spacingbetween A-band centroids. We confirmed the 5.4-nm step. Withsubnanometer precision, however, we find that this value is two timesthat of a more fundamental step size of 2.7 nm. Step sizes were always integer multiples of 2.7 nm, whether the length change was positive ornegative. This value is equal to the linear repeat of actin monomersalong the thin filament, a result that ties dynamic events to molecularstructure and places narrow constraints on any proposed molecular mechanism. 相似文献
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Long-term circulation of vaccine-derived poliovirus that causes paralytic disease 总被引:8,自引:0,他引:8
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Cherkasova EA Korotkova EA Yakovenko ML Ivanova OE Eremeeva TP Chumakov KM Agol VI 《Journal of virology》2002,76(13):6791-6799
Successful implementation of the global poliomyelitis eradication program raises the problem of vaccination against poliomyelitis in the posteradication era. One of the options under consideration envisions completely stopping worldwide the use of the Sabin vaccine. This strategy is based on the assumption that the natural circulation of attenuated strains and their derivatives is strictly limited. Here, we report the characterization of a highly evolved derivative of the Sabin vaccine strain isolated in a case of paralytic poliomyelitis from a 7-month-old immunocompetent baby in an apparently adequately immunized population. Analysis of the genome of this isolate showed that it is a double (type 1-type 2-type 1) vaccine-derived recombinant. The number of mutations accumulated in both the type 1-derived and type 2-derived portions of the recombinant genome suggests that both had diverged from their vaccine predecessors approximately 2 years before the onset of the illness. This fact, along with other recent observations, points to the possibility of long-term circulation of Sabin vaccine strain derivatives associated with an increase in their neurovirulence. Comparison of genomic sequences of this and other evolved vaccine-derived isolates reveals some general features of natural poliovirus evolution. They include a very high preponderance and nonrandom distribution of synonymous substitutions, conservation of secondary structures of important cis-acting elements of the genome, and an apparently adaptive character of most of the amino acid mutations, with only a few of them occurring in the antigenic determinants. Another interesting feature is a frequent occurrence of tripartite intertypic recombinants with either type 1 or type 3 homotypic genomic ends. 相似文献