全文获取类型
收费全文 | 1186篇 |
免费 | 76篇 |
专业分类
1262篇 |
出版年
2024年 | 2篇 |
2023年 | 7篇 |
2022年 | 20篇 |
2021年 | 55篇 |
2020年 | 28篇 |
2019年 | 34篇 |
2018年 | 38篇 |
2017年 | 40篇 |
2016年 | 38篇 |
2015年 | 77篇 |
2014年 | 63篇 |
2013年 | 86篇 |
2012年 | 119篇 |
2011年 | 108篇 |
2010年 | 62篇 |
2009年 | 57篇 |
2008年 | 73篇 |
2007年 | 75篇 |
2006年 | 62篇 |
2005年 | 54篇 |
2004年 | 36篇 |
2003年 | 40篇 |
2002年 | 33篇 |
2001年 | 7篇 |
2000年 | 6篇 |
1999年 | 12篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1980年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1974年 | 3篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有1262条查询结果,搜索用时 15 毫秒
1.
2.
3.
Hydroboration of 5 alpha-cholest-8-ene, 5 alpha,14 beta-cholest-8-ene and 5 alpha,14 beta-cholest-7-ene provides a simple route to oxygenated steroids with 14 beta- and 8 alpha,14 beta- unnatural stereochemistry. 相似文献
4.
A C-glucoside of cholic acid was synthesized by the introduction of an acetyl group at position 3 alpha and direct one-pot C-glucosidation using 2,3,4,6-tetra-O-benzyl-alpha-D-glucopyranosyl chloride. 相似文献
5.
The distance dependence prediction of the Janzen-Connell hypothesis: a meta-analysis 总被引:7,自引:0,他引:7
Laura A. Hyatt Michael S. Rosenberg Timothy G. Howard Gregory Bole Wei Fang Jean Anastasia Kerry Brown Rebecca Grella Katharine Hinman Josepha P. Kurdziel Jessica Gurevitch 《Oikos》2003,103(3):590-602
The Janzen-Connell hypothesis explains the maintenance of tropical diversity through the interacting effects of parent-centered dispersal patterns and distance- and density-dependent propagule survival. These effects were thought to support regular spacing of species within tropical forest, enhancing diversity. One of the predictions of the hypothesis is that seed and seedling survival should improve with increased parental distance. Although there are many independent tests of this hypothesis for individual species, there are few synthetic studies that have brought these data together to test its validity across species. This paper reports the results of a meta-analysis of the effect of distance on enhancing propagule survival, employing an odds-ratio effect size metric. We found no general support for the distance-dependent prediction of the hypothesis, and conclude that further testing to explore this hypothesis as a diversity-maintaining mechanism is unnecessary. However, we did find that distance from parent slightly reduces survivorship in the temperate zone, as contrasted with the tropics, and we saw stronger evidence in support of the hypothesis for seedlings than for seeds. The phenomenon of enhanced propagule survival with distance from the parent may be important for the population biology of particular species, but it is not a general phenomenon across communities, life history stages or life forms. 相似文献
6.
7.
Vassilis Vassiliou Maria Emmanouilidou Andreas Perrakis Evangelia Morou John Vontas Anastasia Tsagkarakou Emmanouil Roditakis 《Insect Science》2011,18(1):30-39
Abstract A comprehensive study on the Bemisia tabaci (biotype B) resistance to neonicotinoid insecticides imidacloprid, acetamiprid and thiamethoxam, and pyrethroid bifenthrin was conducted in Cyprus. The resistance level to eight field‐collected B. tabaci populations was investigated. The activities of enzymes involved in metabolic detoxification and the frequencies of pyrethroid and organophosphates target site resistance mutations were determined. Moderate to high levels of resistance were detected for imidacloprid (resistance factor [RF] 77–392) and thiamethoxam (RF 50–164) while low resistance levels were observed for acetamiprid (RF 7–12). Uniform responses by the Cypriot whiteflies could be observed against all neonicotinoid insecticides. No cross‐resistance between the neonicotinoids was detected as well as no association with the activity of the P450 microsomal oxidases. Only imidacloprid resistance correlated with carboxylesterase activity. Low to extremely high resistance was observed for insecticide bifenthrin (RF 49–1 243) which was associated with the frequency of the resistant allele in the sodium channel gene but not with the activity of the detoxification enzymes. Finally, the F331W mutation in the acetylcholinesterase enzyme ace1 gene was fixed in all B. tabaci populations from Cyprus. 相似文献
8.
Rational siRNA design for RNA interference 总被引:166,自引:0,他引:166
Reynolds A Leake D Boese Q Scaringe S Marshall WS Khvorova A 《Nature biotechnology》2004,22(3):326-330
Short-interfering RNAs suppress gene expression through a highly regulated enzyme-mediated process called RNA interference (RNAi). RNAi involves multiple RNA-protein interactions characterized by four major steps: assembly of siRNA with the RNA-induced silencing complex (RISC), activation of the RISC, target recognition and target cleavage. These interactions may bias strand selection during siRNA-RISC assembly and activation, and contribute to the overall efficiency of RNAi. To identify siRNA-specific features likely to contribute to efficient processing at each step, we performed a systematic analysis of 180 siRNAs targeting the mRNA of two genes. Eight characteristics associated with siRNA functionality were identified: low G/C content, a bias towards low internal stability at the sense strand 3'-terminus, lack of inverted repeats, and sense strand base preferences (positions 3, 10, 13 and 19). Further analyses revealed that application of an algorithm incorporating all eight criteria significantly improves potent siRNA selection. This highlights the utility of rational design for selecting potent siRNAs and facilitating functional gene knockdown studies. 相似文献
9.
Véret J Coant N Berdyshev EV Skobeleva A Therville N Bailbé D Gorshkova I Natarajan V Portha B Le Stunff H 《The Biochemical journal》2011,438(1):177-189
Pancreatic β-cell apoptosis induced by palmitate requires high glucose concentrations. Ceramides have been suggested to be important mediators of glucolipotoxicity-induced β-cell apoptosis. In INS-1 β-cells, 0.4 mM palmitate with 5 mM glucose increased the levels of dihydrosphingosine and dihydroceramides, two lipid intermediates in the de novo biosynthesis of ceramides, without inducing apoptosis. Increasing glucose concentrations to 30 mM amplified palmitate-induced accumulation of dihydrosphingosine and the formation of (dihydro)ceramides. Of note, glucolipotoxicity specifically induced the formation of C(18:0), C(22:0) and C(24:1) (dihydro)ceramide molecular species, which was associated with the up-regulation of CerS4 (ceramide synthase 4) levels. Fumonisin-B1, a ceramide synthase inhibitor, partially blocked apoptosis induced by glucolipotoxicity. In contrast, apoptosis was potentiated in the presence of D,L-threo-1-phenyl-2-palmitoylamino-3-morpholinopropan-1-ol, an inhibitor of glucosylceramide synthase. Moreover, overexpression of CerS4 amplified ceramide production and apoptosis induced by palmitate with 30 mM glucose, whereas down-regulation of CerS4 by siRNA (short interfering RNA) reduced apoptosis. CerS4 also potentiates ceramide accumulation and apoptosis induced by another saturated fatty acid: stearate. Collectively, our results suggest that glucolipotoxicity induces β-cell apoptosis through a dual mechanism involving de novo ceramide biosynthesis and the formation of ceramides with specific N-acyl chain lengths rather than an overall increase in ceramide content. 相似文献
10.