全文获取类型
收费全文 | 454篇 |
免费 | 25篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 6篇 |
2020年 | 6篇 |
2019年 | 4篇 |
2018年 | 8篇 |
2017年 | 3篇 |
2016年 | 7篇 |
2015年 | 14篇 |
2014年 | 18篇 |
2013年 | 26篇 |
2012年 | 29篇 |
2011年 | 24篇 |
2010年 | 21篇 |
2009年 | 16篇 |
2008年 | 20篇 |
2007年 | 33篇 |
2006年 | 15篇 |
2005年 | 17篇 |
2004年 | 11篇 |
2003年 | 30篇 |
2002年 | 18篇 |
2001年 | 19篇 |
2000年 | 9篇 |
1999年 | 9篇 |
1997年 | 3篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 4篇 |
1992年 | 8篇 |
1991年 | 9篇 |
1990年 | 4篇 |
1989年 | 6篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 6篇 |
1985年 | 6篇 |
1984年 | 4篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1980年 | 6篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 6篇 |
1976年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1968年 | 1篇 |
1948年 | 1篇 |
排序方式: 共有479条查询结果,搜索用时 15 毫秒
1.
2.
3.
Complex alternative splicing of acetylcholinesterase transcripts in Torpedo electric organ; primary structure of the precursor of the glycolipid-anchored dimeric form 总被引:16,自引:2,他引:14 下载免费PDF全文
J L Sikorav N Duval A Anselmet S Bon E Krejci C Legay M Osterlund B Reimund J Massouli 《The EMBO journal》1988,7(10):2983-2993
4.
M Dardenne W Savino D Duval D Kaiserlian J Hassid J F Bach 《Journal of immunology (Baltimore, Md. : 1950)》1986,136(4):1303-1308
The influence of adrenals and gonads on the intrathymic production and the circulating level of thymulin was evaluated in young adult mice. Adrenalectomy (Adx) and gonadectomy (Cx) induce a temporary decrease of thymulin serum level. One simultaneously notes, as a compensatory phenomenon, an increase in the thymic content of the hormone-producing cells. The decrease of serum thymulin levels after Adx and Cx is at least partially due to the appearance of low m.w. thymulin-inhibitory molecules. The fact that thymectomy prevents the appearance of these inhibitors suggests that the effects of Adx and Cx could be explained by a negative control by sex hormones of the synthesis or activity of thymulin inhibitors produced or controlled by the thymus. Specific hormone replacement therapy of castrated/adrenalectomized animals normalized thymulin serum level and thymic content. Such correction was also spontaneously observed after 4 mo, suggesting that other mechanisms (e.g., an influence of the hypothalamus-hypophysis axis) might be involved in the endocrine control of thymic hormone secretion. 相似文献
5.
Prostaglandin production by phagocytic cells of the mouse thymic reticulum in culture and its modulation by indomethacin and corticosteroids 总被引:2,自引:0,他引:2
F Homo-Delarche D Duval M Papiernik 《Journal of immunology (Baltimore, Md. : 1950)》1985,135(1):506-512
The production of prostaglandins by phagocytic cells of the thymic reticulum in culture (P-TR) was studied by using high pressure liquid chromatography and radioimmunoassay. Radioimmunologic determinations showed that thromboxane B2 (TXB2), prostaglandin E2 (PGE2), and 6-keto-prostaglandin F1 alpha (6 keto-PGF1 alpha) were the major compounds released into the culture medium, whereas prostaglandin F2 alpha (PGF2 alpha) was only a minor component. Indomethacin and dexamethasone exerted a similar pattern of differential inhibition of the secretion of prostanoids. PGE2 and 6-keto PGF1 alpha productions were markedly decreased by these anti-inflammatory drugs, whereas those of TXB2 and PGF2 alpha were not or were only slightly affected. Experiments performed with an antiglucocorticoid compound (RU 38486) showed that the steroid-induced inhibition of prostanoid secretion is a classical receptor-mediated action. These results demonstrated that phagocytic cells of the thymic reticulum, which resemble the thymic interdigitating cells, produce several types of prostaglandins. Because it has been described that P-TR regulate thymocyte proliferation in vitro via the secretion of both interleukin 1 and PGE2, these results suggest that anti-inflammatory agents may be able to modulate the thymic microenvironment and, consequently, thymocyte proliferation. 相似文献
6.
Juliette Gaëtan Sébastien Halary Maxime Millet Cécile Bernard Charlotte Duval Sahima Hamlaoui Amandine Hecquet Muriel Gugger Benjamin Marie Neha Mehta David Moreira Fériel Skouri-Panet Cynthia Travert Elodie Duprat Julie Leloup Karim Benzerara 《Environmental microbiology》2023,25(3):751-765
The formation of intracellular amorphous calcium carbonates (iACC) has been recently observed in a few cultured strains of Microcystis, a potentially toxic bloom-forming cyanobacterium found worldwide in freshwater ecosystems. If iACC-forming Microcystis are abundant within blooms, they may represent a significant amount of particulate Ca. Here, we investigate the significance of iACC biomineralization by Microcystis. First, the presence of iACC-forming Microcystis cells has been detected in several eutrophic lakes, indicating that this phenomenon occurs under environmental conditions. Second, some genotypic (presence/absence of ccyA, a marker gene of iACC biomineralization) and phenotypic (presence/absence of iACC) diversity have been detected within a collection of strains isolated from one single lake. This illustrates that this trait is frequent but also variable within Microcystis even at a single locality. Finally, one-third of publicly available genomes of Microcystis were shown to contain the ccyA gene, revealing a wide geographic and phylogenetic distribution within the genus. Overall, the present work shows that the formation of iACC by Microcystis is common under environmental conditions. While its biological function remains undetermined, this process should be further considered regarding the biology of Microcystis and implications on the Ca geochemical cycle in freshwater environments. 相似文献
7.
OBJECTIVE: To estimate the incidence rate of sexually transmitted diseases (STDs) among university students and evaluate the associated sociodemographic factors. DESIGN: Mail survey in April 1990. Included in the questionnaire were questions about the subjects'' STD experience since their admission to the university and the type and date of the infection. SUBJECTS: Of the 19,682 undergraduate students 2920 subjects, in 10 groups of 292, were randomly selected. A total of 1731 (59.4%) completed the questionnaire. MAIN OUTCOME MEASURES: Estimated annualized incidence rates of genital human papillomavirus infection and Chlamydia infection. RESULTS: The estimated annualized incidence rates of genital human papillomavirus and Chlamydia infections were 2.2% and 1.5% respectively. Among the students who indicated being infected with genital human papillomavirus 59% were 18 to 21 years old (p < 0.05), 76% were women (p < 0.01) and 69% had more than one sexual partner (p < 0.01). No statistically significant associations were observed between age, sex and Chlamydia infection. On the other hand, 95% of the cases of Chlamydia infection were found among those who had more than one sexual partner (p < 0.01). CONCLUSION: University students continue to have sexual activities at risk for STDs and should be specifically targetted by general practitioners and health services in an effort to slow the spread of STDs. 相似文献
8.
Richard Swartz Loyd West Iouri Boiko Anais Malpica Calum MacAulay Anita Carraro Martial Guillaud Dennis Cox Michele Follen 《Analytical cellular pathology》2003,25(4):193-200
This is a methodological study exploring the use of quantitative histopathology applied to the cervix to discriminate between normal and cancerous (consisting of adenocarcinoma and adenocarcinoma in situ) tissue samples. The goal is classifying tissue samples, which are populations of cells, from measurements on the cells. Our method uses one particular feature, the IODs-Index, to create a tissue level feature. The specific goal of this study is to find a threshold for the IODs-Index that is used to create the tissue level feature. The main statistical tool is Receiver Operating Characteristic (ROC) curve analysis. When applied to the data, our method achieved promising results with good estimated sensitivity and specificity for our data set. The optimal threshold for the IODs-Index was found to be 2.12. 相似文献
9.
Michel Hughes Daniéle Duval Heidy Schmid Patrick Kitabgi Michel Lazdunski Jean-Pierre Vincent 《Life sciences》1982,31(5):437-443
This paper describes the interaction of apamin, the bee venom neurotoxin, with its receptor in the guinea pig colon. The pharmacological activity of the toxin was assayed by measuring its contracting effect on guinea pig colon preparations that had been previously relaxed by neurotensin. The IC50 value of apamin in this bioassay is 7 nM. These pharmacological data are compared to the binding properties of apamin to smooth muscle membranes prepared from guinea pig colon. The highly radiolabeled monoiododerivative of apamin binds to its colon receptor with a dissociation constant . The maximal binding capacity of colonic membranes is 30dfmol/mg of protein. The dissociation constant of the unmodified toxin is 23 pM. The difference between the toxin concentrations that produce half-maximal effects in the binding and pharmacological studies arises from the different experimental conditions used for the two assays. 相似文献
10.
A preparation of photochemically active chloroplasts of Fucus was added to a low-salt medium with high osmolarity (HEPES AMPD buffer, 1M sorbitol). The rate of DCIP reduction (DCIPr) and the variable fluorescence (Fv) of these phaeoplasts were measured and compared with the same activities in spinach chloroplasts. A study of the influence of mono- and divalent-cations showed that salt effects on PS II activity also exist in Fucus.
- Mg++ action on Fv is similar, although noticeably weaker in Fucus than in spinach chloroplasts.
- Na+ has no effect on Fv of Fucus chloroplasts, but its influence on DCIPr is more pronounced than in spinach.
- Mg++ influence on DCIPr is largely dependent upon excitation energy. In subsaturating light (100\2-1000 W m\t-2), Mg++ stimulation increases up to 100 mM, almost doubling the level. In very low wight conditions (3Wm\t02), however, this stimulation saturates at about 10 mM; higher concentrations are no longer effective but do not quench DCIPr noticeably, unlike the case in spinach.