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1.
Transduction and Genetic Homology Between Pseudomonas Species putida and aeruginosa 总被引:4,自引:0,他引:4
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Genetic crosses occur by transduction between the species Pseudomonas aeruginosa and P. putida. The frequency relative to intraspecific transfer is reduced and varies among markers, suggesting that these genomes contain discrete regions of homology and nonhomology. 相似文献
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U. M. X. Sangodkar T. L. Aldrich R. A. Haugland J. Johnson R. K. Rothmel P. J. Chapman A. M. Chakrabarty 《Engineering in Life Science》1989,9(4):301-316
Chlorinated aromatic hydrocarbons are widely used in industry and agriculture, and comprise the bulk of environmental pollutants. Although simple aromatic compounds are biodegradable by a variety of degradative pathways, their halogenated counterparts are more resistant to bacterial attack and often necessitate evolution of novel pathways. An understanding of such evolutionary processes is essential for developing genetically improved strains capable of mineralizing highly chlorinated compounds. This article provides an overview of the genetic aspects of dissimilation of chloroaromatic compounds and discusses the potential of gene manipulation to promote enhanced evolution of the degradative pathways. 相似文献
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Non-pathogenic, environmental strain ofVibrio cholerae, ELTOR Ogawa EW6 carries a copy of the cholera toxin gene in its chromosome. Restriction enzyme digestion followed by Southern
blot analysis revealed that the structure of the cholera toxin gene in this organism is different from that found in the virulent
strains. The xbaI site which has been found to be conserved in the cholera toxin of the virulent strains examined so far,
is absent here. Results of the RNA dot blot analysis indicated that the cholera toxin gene in EW6 is transcribed much less
efficiently compared to the cholera toxin gene present in the virulent strainVibrio cholerae classical Inaba 569B. 相似文献
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Paul J. Chuba Myron A. Leon Amit Banerjee Sunil Palchaudhuri 《Molecular & general genetics : MGG》1989,216(2-3):287-292
Summary
Escherichia coli K12 cells carrying a cloned 1.4 kb HindIII fragment from plasmid ColV2-K94, showed increased survival in guinea pig serum. The recombinant plasmid also conferred group II surface exclusion, i.e. the cells were reduced in recipient ability towards the incoming plasmid R538drd in conjugation experiments. Southern blotting suggested homology with bacteriophage lambda DNA and to the insertion element IS2. Determination of the DNA sequence of the fragment demonstrated the presence of a truncated IS2 (165 bp), separated by 250 bp from a 900 bp stretch of homology with lambda DNA, beginning within the Rz gene and continuing in the rightward direction on the lambda map. A 97 amino acid open reading frame (ORF) adjacent to Rz and on the opposite strand, remained intact in iss, with several amino acid changes. The ORF in iss is preceded by sequences resembling prokaryotic ribosome binding sites and promoters. 相似文献
8.
Bhuwan Joshi Ayan Chakrabarty Christopher Bruot Hannah Ainsworth Gail Fraizer et al. 《Plant Growth Regulation》1989,8(3):286-286
Book Review
Techniques in molecular biologyJ.M. Walker and W. Gaastra (Eds.), vol. 2. London: Croom Helm, 1987. iv + 332 pages. £14.95. ISBN 0-7099-3673-7 相似文献9.
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