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Usefulness of a microimmunodiffusion test for the detection of Penicillium marneffei antigenemia,antibodies, and exoantigens 总被引:1,自引:0,他引:1
Eight sera from culturally-proven cases of penicilliosis marneffei and their corresponding isolates were examined for circulating
antibody(ies) and antigen, and exoantigens, respectively, using a microimmunodiffusion (MID) test. Two of the 8 sera produced
strong precipitins (1-2) when reacted against control Penicillium marneffei antigen (5-week-old shaken cultures at 25 C) in
the presence of control rabbit anti-P. marneffei serum. Five of the 8 sera produced a strong precipitin line when reacted
against control hyperimmune serum to P. marneffei. These five sera, and one additional serum, which tested negative for antibody
to P. marneffei, demonstrated the presence of antigen by reacting only against the anti-P. marneffei serum. Serological evaluations
of the sera revealed that the MID test is capable of detecting antibody and antigen in AIDS patients having penicilliosis
marneffei infections. Exoantigen analysis of the 8 P. marneffei isolates, which were previously identified using this conventional
and time-consuming macro- and micro-morphological characteristics, showed the presence of 1 to 4 specific exoantigens in MID.
With the exoantigen analysis, the identity of all of the isolates was confirmed as P. marneffei. Our studies indicated that
the serological tests are useful for detecting circulating antibody and/or antigen in patients' sera, and that the exoantigen
test is reliable for confirming the identity of P. marneffei cultures.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Sekhon A.S. Padhye A.A. Kaufman L. Garg A.K. Ajello L. Ambrosie E. Panter T. 《Mycopathologia》1997,138(1):1-4
Exoantigenic extracts of 15 isolates belonging to hyalohyphomycosis-causing Beauveria bassiana (1), and Engyodontium album
(1), as well as other species of the genus Beauveria (one isolate each of B. brogniartii, B. densa, B. stephanoderis, B. velata,
B. vermiconia and six isolates of unknown Beauveria species) were studied. Aqueous-merthiolated extracts derived from 10-day-old
Sabouraud's dextrose agar slant cultures (25 °C) were concentrated (25X), and reacted against rabbit anti-B. bassiana serum
in the presence of partially purified homologous antigen (20X) prepared from 5-week-old shaken cultures (30 °C), using a microimmunodiffusion
procedure. Beauveria bassiana reference antigen and antiserum reacted to produce four bands of identity. With the exception
of E. album, which was negative, extracts of the isolates of B. brogniartii, B. densa, B. stephanoderis, B. velata, B. vermiconia
and the unknown Beauveria species all produced 2-4 lines of identity against the homologous anti-B. bassiana serum. These
results suggested that all the species of the genus Beauveria tested were antigenically related to B. bassiana. Engyodontium
album demonstrated antigenic distinctness, however, from B. bassiana and thus supported the validity of this taxon.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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