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Lotfi Mellouli Raoudha Ghorbel Alya Kammoun Monia Mezghani Samir Bejar 《Biotechnology letters》1996,18(7):809-814
Summary A new thermophilic Streptomyces sp. TO1, isolated from Tunisian soil, produced a thermostable alpha-amylase and pullulanase. The gene encoding for the alpha-amylase activity was cloned into the multicopy cloning plasmid pLM1 using S. lividans ZX1 as host strain. The ZX1 / pLM1 strain has the same activity than the initial TO1 strain and about 25 fold higher activity than the ZX1 strain. This alpha-amylase has an optimum of pH and temperature at 6 and 70 °C respectively. 相似文献
3.
Ahsan Ullah Khan 《Luminescence》1995,10(6):329-333
Simple acidification of aqueous alkaline peroxynitrite quantitatively generates singlet (1Δg) molecular oxygen, detected and quantitated spectroscopically (1270 nm). This observation provides a chemical basis for physiological cytotoxicity of ONOO? generated in the diffusion - controlled reaction of cellular NO? and O. The experiments consist of (i) chemical generation of ONOO? from NO? gas and KO2 powder in alkaline aqueous solution; (ii) absorption spectral identification of ONOO? in the near-UV with maximum at 302 nm; (iii) spectroscopic identification of 1O2 by its emission band at 1200–1340 nm with maximum at 1275 nm; and (iv) quantitation of 1O2 generated in ONOO?/H+ reaction by comparison of the chemiluminescence intensity at 1270 nm with that from H2O2/OCl? reaction that generates 1O2 with unit efficiency at alkaline pH. 1O2 was generated with unit efficiency with respect to ONOO? concentration by the ONOO?/H+ reaction. 相似文献
4.
Rotifers were cultured with five different organic and inorganic fertilizers in nursery ponds. Of the fertilizers used, mustard oil cake gave significantly (p < 0.01) higher production of rotifers than that of mohua oil cake followed by cow-dung, wheat bran, mixture of NPK and control. The higher production of rotifers was directly related with the higher doses of fertilizers. Among the rotifer species identified, the abundance of Brachionus caudatus and B. forficula were significantly (p < 0.01) higher than others. Available N, available P, exchangeable K and exchangeable Ca and exchangeable Mg were generally higher in ponds where organic fertilizers were used. Proximate composition of rotifers varied depending on the kinds of fertilizers. The multiple correlations of physico-chemical properties were highly significant (p < 0.01) with the growth and production of B. caudatus (R = 0.995), B. forficula (R = 0.932), Trichocerca capucina (R = 0.917), B. patulus (R = 0.901) and B. angularis (R = 0.892) and simply significant (p < 0.05) in the case of Keratella tropica (R = 0.880), Hexarthra intermadia (R = 0.875), B. calyciflorus (R = 0.864) and Filinia spp. (R = 0.856) contributing 91.20%, 86.86%, 84.09%, 81.18%, 79.57%, 77.44%, 76.56%, 74.65% and 73.27% of total effect of water properties on the growth of these species, respectively. The residual effect of nine different physico-chemical properties of water on the production of rotifers was 78.92% which indicates that these properties of water had only 21.08% influence on the production of rotifers. 相似文献
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Nagib Ahsan Luca Fornelli Fares Z. Najar Sanjeewa Gamagedara Mohammad Robiul Hossan R. Shyama Prasad Rao Ujwal Punyamurtula Andrew Bauer Zhibo Yang Steven B. Foster Maureen A. Kane 《Proteomics》2023,23(20):2300150
Blood serum is arguably the most analyzed biofluid for disease prediction and diagnosis. Herein, we benchmarked five different serum abundant protein depletion (SAPD) kits with regard to the identification of disease-specific biomarkers in human serum using bottom-up proteomics. As expected, the IgG removal efficiency among the SAPD kits is highly variable, ranging from 70% to 93%. A pairwise comparison of database search results showed a 10%–19% variation in protein identification among the kits. Immunocapturing-based SAPD kits against IgG and albumin outperformed the others in the removal of these two abundant proteins. Conversely, non-antibody-based methods (i.e., kits using ion exchange resins) and kits leveraging a multi-antibody approach were proven to be less efficient in depleting IgG/albumin from samples but led to the highest number of identified peptides. Notably, our results indicate that different cancer biomarkers could be enriched up to 10% depending on the utilized SAPD kit compared with the undepleted sample. Additionally, functional analysis of the bottom-up proteomic results revealed that different SAPD kits enrich distinct disease- and pathway-specific protein sets. Overall, our study emphasizes that a careful selection of the appropriate commercial SAPD kit is crucial for the analysis of disease biomarkers in serum by shotgun proteomics. 相似文献
7.
Annual gross primary productivity in mesotrophic Shahidullah Hall pond (Dhaka, Bangladesh) was 1383.35 g C m−2 y−1 (arithmetic mean). Daily primary productivity (between 1.6 and 6.8 g C m−2 d−1 was correlated with chlorophylla, day length and dissolved silica. Chlorophylla related significantly withk, incident light, SRP, alkalinity and conductivity. A negative correlation existed between biomass and rainfall. Productivity,
biomass, conductivity, alkalinity, and SRP increased after mid-winter.k, I
k
andZ
eu varied according seasonally.P
max related directly with temperature. Seasonal variation of ∝
B
was 0.0049–0.0258 mg C (mg chla mmol PAR)−1 m−2. Q10 was 2.12, community respiration 1334.99 g C m−2 y−1, and the underwater light climate 186.43μE m−2 s−1. 相似文献
8.
Experimental data suggest that the B-cell antigen CD20 may play a significant role in the pathogenesis of many diseases including glomerular diseases. These and other findings underpin the central concept of B-cell-depleting therapies that target CD20 antigen as treatments for lupus nephritis, idiopathic membranous nephropathy, focal segmental glomerulosclerosis, cryglobulinemic glomerulonephritis, antibody mediated renal allograft rejection and recurrent glomerulonephritis in renal allograft. Use of rituximab as a B-cell depleting therapy has been associated with clinical improvement and has emerged as a possible adjunct or alternative treatment option in this field of nephrology. 相似文献
9.
Md. Ashekur Rahman Md. Yeamin Hossain Sumaya Tanjin Zannatul Mawa Md. Rabiul Hasan Kazi Ahsan Habib Jun Ohtomi 《Zeitschrift fur angewandte Ichthyologie》2021,37(2):364-366
Length weight relationships (LWR) of five marine fishes were estimated from the Bay of Bengal, Bangladesh. Fish samples were collected occasionally from fisher's catch with gill net (mesh size: 3 cm) during November 2019 to October 2020. Fishing was done overnight. Total length (0.1 cm) and body weight (0.01 g) of each individual were measured. We recorded maximum total length for P. maculatus (55.0 cm) and P. parmatus (39.0 cm). 相似文献
10.
Khan Muhammad Tahir Zeb Muhammad Tariq Ahsan Hina Ahmed Abrar Ali Arif Akhtar Khalid Malik Shaukat Iqbal Cui Zhilei Ali Sajid Khan Anwar Sheed Ahmad Manzoor Wei Dong-Qing Irfan Muhammad 《Archives of microbiology》2021,203(1):59-66
Archives of Microbiology - Severe acute respiratory syndrome virus 2 (SARS-CoV-2) belongs to the single-stranded positive-sense RNA family. The virus contains a large genome that encodes four... 相似文献