排序方式: 共有30条查询结果,搜索用时 62 毫秒
1.
Kadir Yesilbag Levent Aydin Ender Dincer Gizem Alpay A. Onur Girisgin Pelin Tuncer Aykut Ozkul 《Experimental & applied acarology》2013,60(2):253-261
Crimean-Congo hemorrhagic fever (CCHF) is an increasing health concern in Turkey since 2002. There were also some recent human cases from the South Marmara region of Turkey; thus, a tick survey was performed, and possible vector tick species for the CCHF virus were determined in the region. A total of 740 adult ticks were collected from infested livestock from five locations: Çanakkale-Biga, Bursa-Orhaneli, Bursa-Keles, Bal?kesir and Bilecik. Total of 11 tick species from the genera Hyalomma, Rhipicephalus, Dermacentor, Ixodes and Haemaphysalis were identified. Rhipicephalus ticks were dominant in the region; the most frequently observed tick species was R. turanicus, (53.1 %), and only 15.4 % of the identified ticks were H. marginatum. The occurrence of H. rufipes infestation in the region fort he first time. A total of 73 pools of adult ticks were tested with both an antigen-detecting ELISA and RT real-time PCR (RT rt PCR). The presence of the CCHF virus was demonstrated in 9 (12.3 %) of the tested tick pools. Although seven of the tick pools were positive for the CCHF virus with both of the methods, one pool was positive only with RT rt PCR and the other pool was only positive with the ELISA. Positive results were obtained from ticks collected from cattle, sheep and goats from two locations, Bursa-Orhaneli and Bilecik. The CCHF virus was detected in R. turanicus (n = 3), R. bursa (n = 2), H. marginatum (n = 2) and D. marginatus (n = 2) ticks. The results of this study confirm the presence of the CCHF virus and present preliminary data on the vector tick species in the southern Marmara region of Turkey. 相似文献
2.
The dynamics of adenylate kinase of Escherichia coli (AKeco) and its complex with the inhibitor AP(5)A, are characterized by correlating the theoretical results obtained with the Gaussian Network Model (GNM) and the anisotropic network model (ANM) with the order parameters and correlation times obtained with Slowly Relaxing Local Structure (SRLS) analysis of (15)N-NMR relaxation data. The AMPbd and LID domains of AKeco execute in solution large amplitude motions associated with the catalytic reaction Mg(+2)*ATP + AMP --> Mg(+2)*ADP + ADP. Two sets of correlation times and order parameters were determined by NMR/SRLS for AKeco, attributed to slow (nanoseconds) motions with correlation time tau( perpendicular) and low order parameters, and fast (picoseconds) motions with correlation time tau( parallel) and high order parameters. The structural connotation of these patterns is examined herein by subjecting AKeco and AKeco*AP(5)A to GNM analysis, which yields the dynamic spectrum in terms of slow and fast modes. The low/high NMR order parameters correlate with the slow/fast modes of the backbone elucidated with GNM. Likewise, tau( parallel) and tau( perpendicular) are associated with fast and slow GNM modes, respectively. Catalysis-related domain motion of AMPbd and LID in AKeco, occurring per NMR with correlation time tau( perpendicular), is associated with the first and second collective slow (global) GNM modes. The ANM-predicted deformations of the unliganded enzyme conform to the functional reconfiguration induced by ligand-binding, indicating the structural disposition (or potential) of the enzyme to bind its substrates. It is shown that NMR/SRLS and GNM/ANM analyses can be advantageously synthesized to provide insights into the molecular mechanisms that control biological function. 相似文献
3.
4.
Zeynep Nagehan Yuruk Yildirim Sebahat Usta Akgul Harika Alpay Bagdagul Aksu Fatma Savran Oguz Aysel Kiyak Nurver Akinci Sevgi Yavuz Gul Ozcelik Asuman Gedikbasi Ibrahim Gokce Nese Ozkayin Nurdan Yildiz Cemile Pehlivanoglu Nilufer Goknar Seha Saygili Sebahat Tulpar Nuran Kucuk Ilmay Bilge Mehmet Tasdemir Ayse Agbas Ahmet Dirican Sevinc Emre Ahmet Nayir Alev Yilmaz 《Cell stress & chaperones》2021,26(6):973
Various molecular and cellular processes are involved in renal fibrosis, such as oxidative stress, inflammation, endothelial cell injury, and apoptosis. Heat shock proteins (HSPs) are implicated in the progression of chronic kidney disease (CKD). Our aim was to evaluate changes in urine and serum HSP levels over time and their relationships with the clinical parameters of CKD in children. In total, 117 children with CKD and 56 healthy children were examined. The CKD group was followed up prospectively for 24 months. Serum and urine HSP27, HSP40, HSP47, HSP60, HSP70, HSP72, and HSP90 levels and serum anti-HSP60 and anti-HSP70 levels were measured by ELISA at baseline, 12 months, and 24 months. The urine levels of all HSPs and the serum levels of HSP40, HSP47, HSP60, HSP70, anti-HSP60, and anti-HSP70 were higher at baseline in the CKD group than in the control group. Over the months, serum HSP47 and HSP60 levels steadily decreased, whereas HSP90 and anti-HSP60 levels steadily increased. Urine HSP levels were elevated in children with CKD; however, with the exception of HSP90, they decreased over time. In conclusion, our study demonstrates that CKD progression is a complicated process that involves HSPs, but they do not predict CKD progression. The protective role of HSPs against CKD may weaken over time, and HSP90 may have a detrimental effect on the disease course.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12192-021-01239-9. 相似文献
5.
Mustafa Çulha Şaban Kalay Elif Sevim Müberra Pinarbaş Yıldız Baş Rahşan Akpinar Şengül Alpay Karaoğlu 《Biometals》2017,30(6):893-902
Bee disease caused by spore-forming Paenibacillus larvae and Paenibacillus alvei is a serious problem for honey production. Thus, there is an ongoing effort to find an effective agent that shows broad biocidal activity with minimal environmental hazard. In this study, the biocidal effect of maltose reduced silver nanoparticles (AgNPs) is evaluated against American foulbrood and European foulbrood pathogens. The results demonstrate that the maltose reduced AgNPs are excellent short and long-term biocides against P. larvae isolates. The long-term effect suggests that the Ag+ ions are released from the AgNPs with increasing time in a controlled manner. 相似文献
6.
Necla Pehlivan Abdullah Muhammed Yesilyurt Nuran Durmus Sengul Alpay Karaoglu 《Acta Physiologiae Plantarum》2017,39(3):79
Certain number of commercial products has been developed to grow stress resistant plants for the sustainability of agriculture in current era with full of energy dependency and hunger besides global warming. Given the risk of these products on the microbial environment in the rhizosphere and food security of mankind due to the accumulated chemical pollution through the food web, organic fungal plant growth promoting agents might be used. Hence, effects of Trichoderma lixii ID11D (TXD), our local fungal isolate on Zea mays L. cv. Samada 07 as a seed biopriming agent was investigated under dose dependent NaCl stress in this study. Fungus was characterized based on ITS (internal transcribed spacer) regions via clustering approach after isolated from tea plantation area. The higher the NaCl concentration, the more effective the TXD was observed. Improved electron transport rate (ETR), maximum quantum efficiency of PS II (Fv/Fm), the effective quantum yield of PS2 (ΦPS2), photochemical quenching (qP) and decreased non-photochemical quenching (NPQ) was detected in TXD primed plants after NaCl expose. TXD seed biopriming increased the lengths, fresh and dry weights of root/shoots and decreased the lipid peroxidation (MDA) remarkably. Non-enzymatic pathway was found to be more effective than enzymatic one for seed bioprimed plants proven by higher RWC, soluble protein, proline, chlorophyll, carotenoid, and less H2O2 and MDA levels under each dose of NaCl. Detected phenotypic and biochemical improvements pave the way of the potential usage of the formulated fungus biopreperations as a pre-harvest agent in agriculturally important cereals in the future and going beyond lab-based level. 相似文献
7.
8.
Acikgoz Eda Mukhtarova Gunel Alpay Araz Avci Cigir Biray Bagca Bakiye Goker Oktem Gulperi 《Molecular biology reports》2021,48(4):3567-3578
Molecular Biology Reports - Cancer stem cells (CSCs) are a unique population that has been linked to drug resistance and metastasis and recurrence of prostate cancer. The sonic hedgehog (SHH)... 相似文献
9.
Kekecoglu Meral Sonmez Emine Acar Merve Kambur Karaoglu Sengul Alpay 《Biology Bulletin》2022,48(3):S145-S145
Biology Bulletin - An Erratum to this paper has been published: https://doi.org/10.1134/S1062359022330019 相似文献
10.